Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

Abstract

As dogs are good models for in vivo studies, it is interesting to evaluate the behavior of canine gingival fibroblasts (CGF) in vitro, so that these cells could be seeded on a matrix and later studied in vivo. The aim of this study was to perform a morphological, functional and biochemical analysis of CGF, comparing it with human gingival fibroblasts (HGF), as well as to evaluate the change of their characteristics over several passages. Using gingival fibroblasts from 3 dogs and 3 humans in the subculture (Sub), first (P1), third (P3), fifth (P5) and seventh (P7) passages, the following parameters were assessed: cell morphology, spreading, adhesion, viability and total protein content. The results showed no major differences between the passages in terms of morphology and spreading, and a tendency of greater adhesion and viability for HGF when compared with CGF. The total protein content was significantly higher for HGF. HGF exhibited greater functional and biochemical activity in vitro compared to CGF. Higher numbers at Sub were observed for both CGF and HGF in all evaluated parameters. The differences do not prevent the use of CGF for tissue engineering, but its use seems to be more appropriate in the subculture or first passage.

A novel hydrogel scaffold for periodontal ligament stem cells

“…This phenomenon can be explained by a well-known property of fibroblast-like cells that individual cells initially spread out and display a typical morphology with processes only a few hours later. These stages of adhesion were consistently observed by various investigators for gingival fibroblasts [22], periodontal ligament fibroblasts [23], and bone marrow mesenchymal SCs [24]. The increasing impedance in PDLSC cultures after 8–10 h reported in our present experiment suggests that these cells complete the adhesion phase in 10 h and the proliferation phase begins afterward.…”

Biological Interaction Between Human Gingival Fibroblasts and Vascular Endothelial Cells for Angiogenesis: A Co-culture Perspective

“…HGFs culture in different matrices has been studied that has shown promising results in soft tissue regeneration [72][73][74] and exhibit greater functional and biochemical activity in vitro such as increased cell adhesion, cell number and total protein count [75]. Mariotti and Cochran [76] compared the growth characteristics and macromolecular synthesis of HGF and human periodontal ligament fibroblast (HPDLF).…”

The effect of two drug delivery systems in ropivacaine cytotoxicity and cytokine release by human keratinocytes and fibroblasts

“…Fibroblasts are essential cells to tissue repair being one of the first cells to appear in injured sites. The HGF culture is a suitable model to replace in‐vivo tests, as in‐vitro cells have morphologies and spatial distribution similar to the in‐vivo system . These two cell lineages have been used in well‐defined experimental models in several pharmacology studies, which were designed to investigate intracellular signalling pathways and responses to different stimulation …”

“…The HGF culture is a suitable model to replace in-vivo tests, as in-vitro cells have morphologies and spatial distribution similar to the in-vivo system. [23,24] These two cell lineages have been used in welldefined experimental models in several pharmacology studies, which were designed to investigate intracellular signalling pathways and responses to different stimulation. [25,26] The ropi concentrations used in the present study were based on kinetic studies after infiltrative anaesthesia in rat maxilla.…”