Morphological lung differences in an asthma mice model can be entangled applying the concept of fractal geometry to high-resolution flat-panel Computed Tomography images - a preliminary study

Obert, Martin; Hagner-Benes, Stefanie; Höll, Steffen; Alrifai, M; Gizewski, EG; Renz, Harald

doi:10.1055/s-0032-1315484

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“…To model an asthmatic phenotype, an OVA model of acute allergic lung inflammation has been used. 21 Six mice (= asthmatic mice) were sensitized to OVA by three intraperitoneal (ip) injections of 10 µg OVA grade VI (Sigma, Deisenhofen, Germany) adsorbed to 1.5 mg Al(OH) 3 (Pierce, Rockford, IL, USA) diluted in 200 µl phosphate buffered saline (PBS). This primes the immune response toward a Th2 phenotype.…”

Section: Mouse Model Of Acute Allergic Airway Inflammationmentioning

confidence: 99%

Fractal Geometry Enables Classification of Different Lung Morphologies in a Model of Experimental Asthma

et al. 2015

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Animal models represent the basis of our current understanding of the pathophysiology of asthma and are of central importance in the preclinical development of drug therapies. The characterization of irregular lung shapes is a major issue in radiological imaging of mice in these models. The aim of this study was to find out whether differences in lung morphology can be described by fractal geometry. Healthy and asthmatic mouse groups, before and after an acute asthma attack induced by methacholine, were studied. In vivo flat-panel-based high-resolution Computed Tomography (CT) was used for mice's thorax imaging. The digital image data of the mice's lungs were segmented from the surrounding tissue. After that, the lungs were divided § Corresponding author. 1550024-1Fractals Downloaded from www.worldscientific.com by YALE UNIVERSITY on 06/28/15. For personal use only. M. Obert et al.by image gray-level thresholds into two additional subsets. One subset contained basically the air transporting bronchial system. The other subset corresponds mainly to the blood vessel system. We estimated the fractal dimension of all sets of the different mouse groups using the mass radius relation (mrr). We found that the air transporting subset of the bronchial lung tissue enables a complete and significant differentiation between all four mouse groups (mean D of control mice before methacholine treatment: 2.64 ± 0.06; after treatment: 2.76 ± 0.03; asthma mice before methacholine treatment: 2.37 ± 0.16; after treatment: 2.71 ± 0.03; p < 0.05). We conclude that the concept of fractal geometry allows a well-defined, quantitative numerical and objective differentiation of lung shapes -applicable most likely also in human asthma diagnostics.

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Section: Mouse Model Of Acute Allergic Airway Inflammationmentioning

confidence: 99%