Morphological, mechanical and biological assessment of PCL/pristine graphene scaffolds for bone regeneration

Wang, Weiguang; Caetano, Guilherme Ferreira; Chiang, Wei‐Hung; Braz, Ana Letícia; Blaker, Jonny J.; Frade, Marco Andrey Cipriani; Bartolo, Paulo Jorge Da Silva

doi:10.18063/ijb.2016.02.009

Cited by 50 publications

(70 citation statements)

References 29 publications

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“…PCL/pristine graphene pellets were initially prepared by melt blending in three different pristine graphene concentrations (0.25, 0.50, and 0.75 wt %) [28]. Briefly, pure PCL pellets were heated above 70 °C in a bowl to ensure all material is in a molten state prior to pristine graphene flake addition, at the desired concentrations.…”

Section: Methodsmentioning

confidence: 99%

Enhancing the Hydrophilicity and Cell Attachment of 3D Printed PCL/Graphene Scaffolds for Bone Tissue Engineering

et al. 2016

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Scaffolds are physical substrates for cell attachment, proliferation, and differentiation, ultimately leading to the regeneration of tissues. They must be designed according to specific biomechanical requirements, i.e., certain standards in terms of mechanical properties, surface characteristics, porosity, degradability, and biocompatibility. The optimal design of a scaffold for a specific tissue strongly depends on both materials and manufacturing processes, as well as surface treatment. Polymeric scaffolds reinforced with electro-active particles could play a key role in tissue engineering by modulating cell proliferation and differentiation. This paper investigates the use of an extrusion-based additive manufacturing system to produce poly(ε-caprolactone) (PCL)/pristine graphene scaffolds for bone tissue applications and the influence of chemical surface modification on their biological behaviour. Scaffolds with the same architecture but different concentrations of pristine graphene were evaluated from surface property and biological points of view. Results show that the addition of pristine graphene had a positive impact on cell viability and proliferation, and that surface modification leads to improved cell response.

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Section: Methodsmentioning

confidence: 99%

Enhancing the Hydrophilicity and Cell Attachment of 3D Printed PCL/Graphene Scaffolds for Bone Tissue Engineering

et al. 2016

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“…Synthetic nano HA (particle size ≤ 200 nm) was purchased from Sigma-Aldrich (Gillingham, UK), and used without further modifications. Pristine graphene and GO were synthetized at the Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, and details of the process can be found elsewhere (Wang et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

Advanced mechanical and thermal characterization of 3D bioextruded poly(e-caprolactone)-based composites

Wang

Domingos

Scenini

2018

RPJ

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Purpose-The main purpose of the present work is to study the effect of nano hydroxyapatite (HA) and graphene oxide (GO) particles on thermal and mechanical performances of 3D printed poly(εcaprolactone) (PCL) filaments used in Bone Tissue Engineering (BTE). Design/methodology/approach-Raw materials were prepared by melt blending, followed by 3D printing via 3D Discovery (regenHU Ltd., CH) with all fabricating parameters kept constant. Filaments, including pure PCL, PCL/HA, and PCL/GO, were tested under the same conditions. Several techniques were used to mechanically, thermally, and microstructurally evaluate properties of these filaments, including Differential Scanning Calorimetry (DSC), tensile test, nano indentation, and Scanning Electron Microscope (SEM). Findings-Results show that both HA and GO nano particles are capable of improving mechanical performance of PCL. Enhanced mechanical properties of PCL/HA result from reinforcing effect of HA, while a different mechanism is observed in PCL/GO, where degree of crystallinity plays an important role. In addition, GO is more efficient at enhancing mechanical performance of PCL compared with HA. Originality/value-For the first time, a systematic study about effects of nano HA and GO particles on bioactive scaffolds produced by Additive Manufacturing (AM) for bone tissue engineering applications is conducted in this work. Mechanical and thermal behaviors of each sample, pure PCL, PCL/HA and PCL/GO, are reported, correlated, and compared with literature.

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“…According to literature [15,16], SEP technique was proved to be promising in the field of bone tissue engineering since the fabricated structures simulate bone tissue and help in osteoblast culture. The skeletal system represents a body scaffold since it provides structure to the body, protects internal organs, and simultaneously acting as a calcium and phosphate reservoir [17].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Characterization of Polyetherimide Electrospun Scaffolds Modified with Graphene Nano-Platelets and Hydroxyapatite Nano-Particles

Kostopoulos

Κοτρώτσος

Fouriki

et al. 2020

IJMS

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Solution electrospinning process (SEP) is a versatile technique for generating non-woven fibrous materials intended to a wide range of applications. One of them is the production of fibrous and porous scaffolds aiming to mimic bone tissue, as artificial extracellular matrices (ECM). In the present work, pure and nano-modified electrospun polyetherimide (PEI) scaffolds have been successfully fabricated. The nano-modified ones include (a) graphene nano-platelets (GNPs), (b) hydroxyapatite (HAP), and (c) mixture of both. After fabrication, the morphological characteristics of these scaffolds were revealed by using scanning electron (SEM) and transmission electron (TEM) microscopies, while porosity and mean fiber diameter were also calculated. In parallel, contact angle experiments were conducted so that the hydrophilicity level of these materials to be determined. Finally, the mechanical performance of the fabricated scaffolds was investigated by conducting uniaxial tensile tests. Ιn future work, the fabricated scaffolds will be further utilized for investigation as potential candidate materials for cell culture with perspective in orthopedic applications.

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Morphological, mechanical and biological assessment of PCL/pristine graphene scaffolds for bone regeneration

Cited by 50 publications

References 29 publications

Enhancing the Hydrophilicity and Cell Attachment of 3D Printed PCL/Graphene Scaffolds for Bone Tissue Engineering

Enhancing the Hydrophilicity and Cell Attachment of 3D Printed PCL/Graphene Scaffolds for Bone Tissue Engineering

Advanced mechanical and thermal characterization of 3D bioextruded poly(e-caprolactone)-based composites

Fabrication and Characterization of Polyetherimide Electrospun Scaffolds Modified with Graphene Nano-Platelets and Hydroxyapatite Nano-Particles

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