Morphological modification by tubercle bacilli: no time for denial

Velayati, Ali Akbar; Farnia, Parissa; Masjedi, Mohammad Reza; Жавнерко, Г. К.; Ghanavi, Jalaledin; Poleschuyk, Nikolai Nikolaevich

doi:10.3855/jidc.2266

Cited by 9 publications

(5 citation statements)

References 8 publications

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“…This issue has been well described by Velayati et al [16]. In the present study, stained fragments and other shapes indicate morphological alterations of TB bacilli in the tissue.…”

Section: Discussionsupporting

confidence: 87%

Histopathological Findings in Immunohistological Staining of the Granulomatous Tissue Reaction Associated with Tuberculosis

Karimi

Shamaei

Pourabdollah

et al. 2014

Tuberculosis Research and Treatment

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Purpose. The histological diagnosis of Mycobacterium tuberculosis (MTB) remains a diagnostic challenge despite different methods. Immunohistochemistry (IHC) not only could confirm granulomatous tissue involvement but also can demonstrate MTB antigen immunolocalization. This study tries to clarify the details of immunohistochemical staining for MTB with pAbBCG. Materials/Methods. Twenty-three confirmed TB granulomatous tissue samples were studied by Ziehl-Neelsen and immunohistochemistry (IHC) staining with pAbBCG. Samples were selected from the archive of the Department of Pathology, National Research Institute of Tuberculosis and Lung Disease, Tehran, Iran. Results. IHC staining was positive in all samples, whereas Ziehl-Neelsen was positive in 9 cases out of 23 (39.1%). Tissue types used were pleural tissue, lymph nodes, and lung tissue. IHC showed positive coarse granular cytoplasmic and round, fragmented bacillary staining. In this study, epithelioid cells clearly showed more positive staining at the periphery of the granuloma rather than the center of granuloma. There is also positive staining in endothelial cells, fibroblasts, plasma cells, lymphocytes, and macrophages outside the granuloma. Conclusion. Considering the criteria of positive immunohistochemical staining of TB granulomatous reactions, this stain not only highlights the presence of mycobacterial antigens for tissue diagnosis, but also could morphologically localize its distribution in different cells.

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“…This issue has been well described by Velayati et al [16]. In the present study, stained fragments and other shapes indicate morphological alterations of TB bacilli in the tissue.…”

Section: Discussionsupporting

confidence: 87%

Histopathological Findings in Immunohistological Staining of the Granulomatous Tissue Reaction Associated with Tuberculosis

Karimi

Shamaei

Pourabdollah

et al. 2014

Tuberculosis Research and Treatment

View full text Add to dashboard Cite

show abstract

“…In few cases of spinal tuberculosis, intact bacilli were not seen but globular acid-fast structures were noted. In some studies, such morphological alterations have been mentioned [10,11]. This strengthens our finding and our view that Mycobacterium tuberculosis gets altered in tissues causing difficulty in visualization by ZN but gets detected by GeneXpert and culture.…”

Section: Discussionsupporting

confidence: 89%

Down to the Ground Zero: Perspective From Tissue Diagnosis in Neurological Tb

Maiti¹,

Banerjee²,

Das³

2020

IJCBR

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BACKGROUND/AIM Central nervous system tuberculosis (CNS-TB), a severe form of extrapulmonary tuberculosis presents with non specific symptom leading to delayed diagnosis and increased morbidity. This study compares different modalities of diagnosis of CNS-TB [Multiplex real-time PCR/ GeneXpert, TB culture, Ziehl-Neelsen staining( Z-N staining) and histopathology]. METHOD This is a cross sectional observational study conducted in the Department of Neuropathology from October 2016 to May 2019. Total 128 brain and spinal cases which were clinically suggestive of TB and operated were evaluated. RESULT Granuloma, giant cell and necrosis were less evident in spinal TB compared to brain. GeneXpert and culture positivity were higher(100%) in tissue having poorly formed granuloma compared to well formed granuloma. Z-N stain has poor sensitivity (around 32%). Compared to culture GeneXpert was 100% sensitive and 96% specific in brain TB( detected from tissue) and 87% and 89% in spinal TB respectively. CONCLUSION Combined together all these modalities provide a wholistic approach so that not a single case of tuberculosis is missed.

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“…This issue has been well described by Velayati et al [14]. A similar study [15] mentioned the presence of mycobacterium and intracellular mycobacterium material outside the granuloma and in macrophages and even lymphocytic fragments around the granuloma.…”

Section: Discussionmentioning

confidence: 78%

Assessment of the histopathological prototypes of the pleural fluid and granulomatous tissue reaction associated with pleural tuberculosis

Abumossalam

Moawd

Shebl

2014

Egyptian Journal of Chest Diseases and Tuberculosis

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Purpose: The aim of this study is to differentiate between the histopathological features of pleural tuberculosis both free and encysted forms of pleural effusion as regards pleural fluid cytology and pleural tissue granulomatous tissue reaction and assess the effects of combined microbial infection on histopathological and clinical residuals.Patients and methods: One hundred and ninety patients were confirmed to be tuberculous by pleural tissue biopsy. Pleural fluid cytopathology and TB granulomatous tissue biopsy samples were studied.Results: Within histopathological features in TB pleural effusion is typical granulomatous TB reaction that was prevalent in both free and encysted forms of pleural effusion with high percentage (97% and 87.5% respectively). On the other hand, lymphocytic exudative smear was predominant in the free TB effusion group than both inflammatory and hemorrhagic ones (74.7% versus 23.5% and 1.8% respectively) while the encysted effusion group presented higher predominance to inflammatory exudative smear than other types (54.2% versus 41.7% and 4.2% respectively). Moderate cellularity was higher in percentage in both free and encysted groups 77.7% and 62.5% respectively. Also, langerhans cells prevailed in both effusion types. Lastly, non TB culture was present in all cases of encysted pleural effusions but accounted only for 3% of free effusion cases.Conclusion: Differentiation between free and encysted forms of pleural tuberculous effusion as regards effusion cytopathological criteria and pleural tissue histopathological patterns represent an important support in understanding the pathogenesis of pleural tuberculous tissue reactions and other positive cultural diagnosis can aggravate TB granulomatous reactions.

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Morphological modification by tubercle bacilli: no time for denial

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Cited by 9 publications

References 8 publications

Histopathological Findings in Immunohistological Staining of the Granulomatous Tissue Reaction Associated with Tuberculosis

Histopathological Findings in Immunohistological Staining of the Granulomatous Tissue Reaction Associated with Tuberculosis

Down to the Ground Zero: Perspective From Tissue Diagnosis in Neurological Tb

Assessment of the histopathological prototypes of the pleural fluid and granulomatous tissue reaction associated with pleural tuberculosis

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