Morphological, molecular and biological characterization of Esteya vermicola, a nematophagous fungus isolated from intercepted wood packing materials exported from Brazil

Wang, Xuan; Wang, Tingting; Wang, Jincheng; Guan, Tinglong; Li, Hongmei

doi:10.1016/j.myc.2014.01.002

Cited by 17 publications

(19 citation statements)

References 15 publications

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“…Esteyavermicola is known only from an asexual, Hyalorhinocladiella -like state producing lunate and bacilliform conidia (Liou et al 1999, Kubátová et al 2000, Wang et al 2009, 2014) that we also observed in various strains of E.vermicola with a different origin (Taiwan, Korea, Czech Republic). Our strain was identified as E.vermicola based on phylogenetic inferences and morphological characters.…”

Section: Morphology and Taxonomymentioning

confidence: 50%

“…The size of the lunate conidia of our strains (mostly 10 - 12 × 3.4 - 4.5 μm) was similar to that reported for E.vermicola , viz. 9.9–11.9 × 3.4–4.5 μm vs 8.2-11.1 × 3.5–3.7 μm (Taiwan, Liou et al 1999), 9.3–12.4 × 3.0–3.2 μm (Czech Republic, Kubátová et al 2000), 7.7–12.1 × 3.0–3.8 μm (Korea, Wang et al 2009) or 8.7–11.9 × 3.0–3.6 μm (Brazil, Wang et al 2014).…”

Section: Morphology and Taxonomymentioning

confidence: 99%

“…The species was originally isolated from Japanese black pine infected by the pinewood nematode Bursaphelenchusxylophilus , in Taiwan (Liou et al 1999). Since then, its distribution range has been extended to Japan and Korea, Europe (Czech Republic, Italy) and both North (USA) and South America (Brazil) (Liou et al 1999, Kubátová et al 2000, Wang et al 2009, 2014, Li et al 2018). This species is associated with various vectors, including the pinewood nematode, Oxoplatypusquadridentatus and the bark beetle Scolytusintricatus .…”

Section: Morphology and Taxonomymentioning

confidence: 99%

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Differential patterns of ophiostomatoid fungal communities associated with three sympatric Tomicus species infesting pines in south-western China, with a description of four new species

Wang¹,

Wang²,

Fu³

et al. 2019

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Bark beetles and their associated fungi, which cause forest decline and sometimes high mortality in large areas around the world, are of increasing concern in terms of forest health. ThreeTomicusspp. (T.brevipilosus,T.minorandT.yunnanensis) infect branches and trunks ofPinusyunnanensisandP.kesiyain Yunnan Province, in south-western China.Tomicusspp. are well known as vectors of ophiostomatoid fungi and their co-occurrence could result in serious ecological and economic impact on local forest ecosystems. Nonetheless, knowledge about their diversity, ecology, including pathogenicity and potential economic importance is still quite rudimentary. Therefore, an extensive survey of ophiostomatoid fungi associated with theseTomicusspecies infestingP.yunnanensisandP.kesiyawas carried out in Yunnan. Seven hundred and seventy-two strains of ophiostomatoid fungi were isolated from the adult beetles and their galleries. The strains were identified based on comparisons of multiple DNA sequences, including the nuclear ribosomal large subunit (LSU) region, the internal transcribed spacer regions 1 and 2, together with the intervening 5.8S gene (ITS) and the partial genes of β-tubulin (TUB2), elongation factor 1α (TEF1-α) and calmodulin (CAL). Phylogenetic analyses were performed using maximum parsimony (MP) as well as maximum likelihood (ML). Combinations of culture features, morphological characters and temperature-dependent growth rates were also employed for species identification. Eleven species belonging to five genera were identified. These included six known species,Esteyavermicola,Leptographiumyunnanense,Ophiostomabrevipilosi,O.canum,O.minusandO.tingensand four novel taxa, described asGraphilbumanningense,O.aggregatum,SporothrixpseudoabietinaandS.macroconidia. A residual strain was left unidentified asOphiostomasp. 1. The overall ophiostomatoid community was by far dominated by three species, representing 87.3% of the total isolates; in decreasing order, these wereO.canum,O.brevipilosiandO.minus. Furthermore, the ophiostomatoid community of each beetle, although harbouring a diversity of ophiostomatoid species, was differentially dominated by a single fungal species;Ophiostomacanumwas preferentially associated with and dominated the ophiostomatoid community ofT.minor, whereasO.brevipilosiandO.minuswere exclusively associated with and dominated the ophiostomatoid communities ofT.brevipilosusandT.yunnanensis, respectively. Eight additional species, representing the remaining 12.7% of the total isolates, were marginal or sporadic. These results suggested that sympatricTomicuspopulations are dominated by distinct species showing some level of specificity or even exclusivity.

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Section: Morphology and Taxonomymentioning

confidence: 50%

Section: Morphology and Taxonomymentioning

confidence: 99%

Section: Morphology and Taxonomymentioning

confidence: 99%

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Differential patterns of ophiostomatoid fungal communities associated with three sympatric Tomicus species infesting pines in south-western China, with a description of four new species

Wang¹,

Wang²,

Fu³

et al. 2019

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“…For example, a representative of the genus Ceratocystiopsis was detected in this study but not obtained from culture-dependent studies. Although only one strain of the fungus was found, Esteya vermicola was obtained in culture-dependent studies but was not detected in the HTS analysis; E. vermicola is widely distributed worldwide [49][50][51][52], but invariably only one strain is obtained. We speculate that because of the limited number of reads generated, the HTS analysis was unable to help with precise detection of Esteya.…”

Section: Discussionmentioning

confidence: 99%

Epibiotic Fungal Communities of Three Tomicus spp. Infesting Pines in Southwestern China

et al. 2019

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The association between insects and fungi has evolved over millions of years and is ubiquitous in nature. This symbiotic relationship holds critical implications for both partners, the insects and the associated microbes. Numerous fungi are externally allied with bark beetles and form a close symbiosis, but the community structures of these fungi are largely unknown. In Yunnan Province in southwestern China, the beetles Tomicus yunnanensis, T. minor, and T. brevipilosus are major forest pests that cause large losses of two indigenous pines, Pinus yunnanensis and P. kesiya. In this study, we used the Illumina MiSeq PE300 platform to process 48 samples of epibiotic fungal communities pooled from 1348 beetles; the beetles were collected during both the branch- and trunk-infection sections from five locations across Yunnan Province. Considerably greater species richness was detected using high-throughput sequencing of amplified internal transcribed spacer 1 (ITS1) ribosomal DNA (rDNA) libraries than previously documented by using culture-dependent methods. In total, 1,413,600 reads were generated, and a 97% sequence-similarity cutoff produced eight phyla, 31 classes, 83 orders, 181 families, 331 genera, 471 species, and 1157 operational taxonomic units (OTUs), with 659, 621, and 609 OTUs being confined to T. yunnanensis, T. minor, and T. brevipilosus, respectively. Tomicus yunnanensis, T. minor, and T. brevipilosus had the similar OTUs richness and evenness of fungal communities in Yunnan Province; nevertheless, the two fungal community compositions associated with T. yunnanensis and T. minor were structurally similar to each other but distinct from that associated with T. brevipilosus. Lastly, the results of principal co-ordinates analysis suggested that epibiotic fungal community structures of the three Tomicus spp. were conditioned strongly by the locations and pine hosts but weakly by beetle species and infection sections. Our findings provide baseline knowledge regarding the epibiotic fungal communities of three major Tomicus spp. in southwestern China.

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“…Esteya vermicola is the first recorded endoparasitic fungus of PWN [4]. The E. vermicola strains were isolated from infected PWNs, soil, or dead trees [4][5][6][7]. It is well known that E. vermicola has high infectivity against PWN, and the species therefore has been widely used as a biological control agent against PWD in the field [8].…”

Section: Introductionmentioning

confidence: 99%

The specialization ofEsteya vermicolahyphae in infection toBursaphelenchus xylophilusand its colonization of pine tree

Wang

Yin

Gao

et al. 2020

Preprint

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Pine wilt disease (PWD) caused by the nematode Bursaphelenchus xylophilus is a serious problem on pines, and there is currently no effective control strategy for this disease. Although the endoparasitic fungus Esteya vermicola showed great effectiveness in controlling pine wilt disease, the colonization patterns of the host pine tree xylem by this fungus are unknown. To investigate the colonization patterns of pine xylem by this fungus, the species Pinus koraiensis grown in a greenhouse was used as an experimental host tree. The fungal colonization of healthy and wilting pine trees by E. vermicola was quantified using PCR with a TaqMan probe, and a green fluorescence protein (GFP) transformant was used for visualization. The results reported a specific infection approach used by E. vermicola to infect B. xylophilus and specialized fungal parasitic cells in PWN infection. In addition, the inoculated blastospores of E. vermicola germinated and grew inside of healthy pine xylem, although the growth rate was slow. Moreover, E. vermicola extended into the pine xylem following spray inoculation of wounded pine seedling stems, and a significant increase in fungal quantity was observed in response to B. xylophilus invasion. An accelerated extension of E. vermicola colonization was shown in PWN-infected wilting pine trees, due to the immigration of fungal-infected PWNs. Our results provide helpful knowledge about the extension rate of this fungus in healthy and wilting PWN-susceptible pine trees in the biological control of PWD and will contribute to the development of a management method for PWD control in the field.

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Morphological, molecular and biological characterization of Esteya vermicola, a nematophagous fungus isolated from intercepted wood packing materials exported from Brazil

Cited by 17 publications

References 15 publications

Differential patterns of ophiostomatoid fungal communities associated with three sympatric Tomicus species infesting pines in south-western China, with a description of four new species

Differential patterns of ophiostomatoid fungal communities associated with three sympatric Tomicus species infesting pines in south-western China, with a description of four new species

Epibiotic Fungal Communities of Three Tomicus spp. Infesting Pines in Southwestern China

The specialization ofEsteya vermicolahyphae in infection toBursaphelenchus xylophilusand its colonization of pine tree

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