Morphological, molecular and functional differences of adult bone marrow- and adipose-derived stem cells isolated from rats of different ages

Mantovani, Cristina; Raimondo, Stefania; Haneef, Maryam S.; Geuna, Stefano; Terenghi, Giorgio; Shawcross, Susan; Wiberg, Mikael

doi:10.1016/j.yexcr.2012.05.008

Cited by 32 publications

(35 citation statements)

References 67 publications

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“…Moreover, doubling time, telomere length, the osteogenic and chondrogenic differentiation capacity, as well as osteogenic paracrine activity were also found to be similar among ADSC from adult donors of different ages (Ding et al 2013;Wu et al 2013). Consistently, another experimental study investigating the impact of donor age on the function of adipose-derived stem cells also demonstrated that aged ADSC from rats still retained potential to support axon regeneration (Mantovani et al 2012). One interesting finding is that ADSC from older donors were found to exhibit compromised adipogenic potential that actually favors their application in regeneration therapy (Ding et al 2013).…”

Section: Source-and Donor-dependent Variability In Adsc Qualitysupporting

confidence: 58%

Transplantation of Adipose-Derived Stem Cells in Stroke

Sun

2014

Cellular Therapy for Stroke and CNS Injuries

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Section: Source-and Donor-dependent Variability In Adsc Qualitysupporting

confidence: 58%

Transplantation of Adipose-Derived Stem Cells in Stroke

Sun

2014

Cellular Therapy for Stroke and CNS Injuries

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“…These cells lost the beneficial effects on anti-Thy1.1 nephritis seen in MSCs obtained from healthy subjects. Limited regenerative potential of MSCs obtained from older donors was also associated with both morphological and functional changes in mitochondria [53,54]. hTERT-immortalized bone marrow-derived MSCs from patients with Parkinson's disease showed decreased mitochondrial complex I, II, and IV activities [55].…”

Section: Regulators P53 and P16mentioning

confidence: 99%

Umbilical Cord Mesenchymal Stromal Cells Affected by Gestational Diabetes Mellitus Display Premature Aging and Mitochondrial Dysfunction

Kim

Piao

Pak

et al. 2015

Stem Cells and Development

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Human umbilical cord mesenchymal stromal cells (hUC-MSCs) of Wharton's jelly origin undergo adipogenic, osteogenic, and chondrogenic differentiation in vitro. Recent studies have consistently shown their therapeutic potential in various human disease models. However, the biological effects of major pregnancy complications on the cellular properties of hUC-MSCs remain to be studied. In this study, we compared the basic properties of hUC-MSCs obtained from gestational diabetes mellitus (GDM) patients (GDM-UC-MSCs) and normal pregnant women (N-UC-MSCs). Assessments of cumulative cell growth, MSC marker expression, cellular senescence, and mitochondrial function-related gene expression were performed using a cell count assay, senescence-associated b-galactosidase staining, quantitative real-time reverse transcription-polymerase chain reaction, immunoblotting, and cell-based mitochondrial functional assay system. When compared with N-UCMSCs, GDM-UC-MSCs showed decreased cell growth and earlier cellular senescence with accumulation of p16 and p53, even though they expressed similar levels of CD105, CD90, and CD73 MSC marker proteins. GDM-UC-MSCs also displayed significantly lower osteogenic and adipogenic differentiation potentials than N-UC-MSCs. Furthermore, GDM-UC-MSCs exhibited a low mitochondrial activity and significantly reduced expression of the mitochondrial function regulatory genes ND2, ND9, COX1, PGC-1a, and TFAM. Here, we report intriguing and novel evidence that maternal metabolic derangement during gestation affects the biological properties of fetal cells, which may be a component of fetal programming. Our findings also underscore the importance of the critical assessment of the biological impact of maternal-fetal conditions in biological studies and clinical applications of hUC-MSCs.

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“…914,000) 2013). Mantovani et al (2012) described ultra-structure of ATSCs, however, as a source of these cells served the adipose tissue obtained from rats. The authors described some ultra-structural differences depending on whether the cells originated from young or older individuals.…”

Section: Discussionmentioning

confidence: 99%

Ultra-structural morphology of long-term cultivated white adipose tissue-derived stem cells

et al. 2015

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White adipose tissue was long perceived as a passive lipid storage depot but it is now considered as an active and important endocrine organ. It also harbours not only adipocytes and vascular cells but also a wide array of immunologically active cells, including macrophages and lymphocytes, which may induce obesity-related inflammation. Recently, adipose tissue has been reported as a source of adult mesenchymal stem cells with wide use in regenerative medicine and tissue engineering. Their relatively non-complicated procurement and collection (often performed as liposuction during aesthetic surgery) and grand plasticity support this idea even more. We focused our research on exploring the issues of isolation and long-term cultivation of mesenchymal stem cells obtained from adipose tissue. Ultra-structural morphology of the cells cultivated in vitro has been studied and analysed in several cultivation time periods and following serial passages--up to 30 passages. In the first passages they had ultra-structural characteristics of cells with high proteosynthetic activity. Within the cytoplasm, big number of small lipid droplets and between them, sparsely placed, small and inconspicuous, electron-dense, lamellar bodies, which resembled myelin figures were observed. The cells from the later passages contained high number of lamellar electron-dense structures, which filled out almost the entire cytoplasm. In between, mitochondria were often found. These bodies were sometimes small and resembled myelin figures, but several of them reached huge dimensions (more than 1 µm) and their lamellar structure was not distinguishable. We did not have an answer to the question about their function, but they probably represented the evidence of active metabolism of lipids present in the cytoplasm of these cells or represented residual bodies, which arise after the breakdown of cellular organelles, notably mitochondria during long-term cultivation.

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Morphological, molecular and functional differences of adult bone marrow- and adipose-derived stem cells isolated from rats of different ages

Cited by 32 publications

References 67 publications

Transplantation of Adipose-Derived Stem Cells in Stroke

Transplantation of Adipose-Derived Stem Cells in Stroke

Umbilical Cord Mesenchymal Stromal Cells Affected by Gestational Diabetes Mellitus Display Premature Aging and Mitochondrial Dysfunction

Ultra-structural morphology of long-term cultivated white adipose tissue-derived stem cells

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