Morphological, pathological and molecular characterization of Phytophthora colocasiae responsible for taro leaf blight disease in India

Nath, Vishnu Sukumari; Hegde, Vinayaka; Jeeva, M. L.; Misra, R. S.; Veena, S; Raj, Mithun; Sankar, Darveekaran Sree

doi:10.1007/s12600-014-0422-5

Cited by 11 publications

(10 citation statements)

References 29 publications

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“…The isolation and maintenance of the cultures were performed as previously described (Nath et al. ). The isolates were identified to the species level using internal transcribed spacer (ITS) amplification and sequencing (Nath et al.…”

Section: Methodsmentioning

confidence: 99%

“…The isolates were identified to the species level using internal transcribed spacer (ITS) amplification and sequencing (Nath et al. ).…”

Section: Methodsmentioning

confidence: 99%

“…These strains were isolated from leaf blight symptomatic samples obtained from different regions of India. The isolation and maintenance of the cultures were performed as previously described (Nath et al 2014). The isolates were identified to the species level using internal transcribed spacer (ITS) amplification and sequencing (Nath et al 2014).…”

Section: Pathogenmentioning

confidence: 99%

“…Moreover, fungicides pose a risk of resistance development in the pathogen population (Cohen and Coffey ), especially in pathogen populations with a higher evolutionary potential such as P. colocasiae (Nath et al. ). Therefore, breeding and the deployment of resistant cultivars are the most practical and environment‐friendly strategy for disease control.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, this study reports the development of a rapid, simple and repeatable assay that can be used to screen large numbers of taro cultivars for resistance to P. colocasiae. ulations with a higher evolutionary potential such as P. colocasiae (Nath et al 2014). Therefore, breeding J Phytopathol 164 (2016) 520-527 Ó 2016 Blackwell Verlag GmbH 520 J Phytopathol…”

mentioning

confidence: 99%

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A Rapid and Efficient Method for In Vitro Screening of Taro for Leaf Blight Disease Caused by Phytophthora colocasiae

Nath

Basheer

Jeeva

et al. 2016

Journal of Phytopathology

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Taro leaf blight caused by Phytophthora colocasiae presents the single biggest constraint for taro cultivation globally. To accelerate breeding and selection for disease resistance to leaf blight, it is important to develop bioassays which could differentiate resistant and susceptible cultivars efficiently. In this study, thirty taro accessions and four released cultivars were evaluated for resistance to leaf blight using a modified floating leaf disc assay. A novel method for mass production of P. colocasiae zoospores was developed and used as inoculum for the assay. There were significant (P < 0.05) differences among accessions in their response to P. colocasiae infection in the detached leaf assay. The accessions could be efficiently classified into various resistance groups based on a 0-4 score. Also, the assay results were consistent with the field evaluation scores of taro accessions. Thus, this study reports the development of a rapid, simple and repeatable assay that can be used to screen large numbers of taro cultivars for resistance to P. colocasiae.

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Section: Methodsmentioning

confidence: 99%

“…The isolates were identified to the species level using internal transcribed spacer (ITS) amplification and sequencing (Nath et al. ).…”

Section: Methodsmentioning

confidence: 99%