Morphological patterns in Petunia hybrida plants regenerated from tissue cultures and differing by their ploidy

Santos, Rozely Ferreira dos; Handro, Walter

doi:10.1007/bf00281849

Cited by 14 publications

(8 citation statements)

References 15 publications

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“…This is in agreement with the findings ofHermsen and DeBoer (1971). Floral characteristics (corolla radius, corolla width and anther length) were even more diagnostic of ploidy level which supports the association of flower dimensions with polyploidization (Eigsti and Dustin, 1955;Narayanaswamy and Chandy, 1971;Santos and Handro, 1983). The discriminatory power ofleaflet dimensions was less than that ofthe floral characteristics, as for Petunia (Santos and Handro, 1983).…”

supporting

confidence: 53%

“…Early recognition ofmonoploids would hasten the progress of the program. Correlation between ploidy level and various morphological characteristics of plants regenerated from tissue culture has been observed in several Solanaceous species (Tsikov, Zagorska and Tsikova, 1974;Sree Ramulu et aI., 1976;Fassuliotis, 1977;Hell, 1979;Santos and Handro, 1983). The objectives ofthis study were: I) to determine if multivariate statistics, i.e., canonical discriminant analysis and cluster analysis, can be used to identify a set of easily measurable morphological characteristics to diagnose the ploidy level of anther-derived plants of Solanum phureja; and 2) to quantify the variation within each ploidy group for identification of exceptional genotypes for use in further breeding.…”

mentioning

confidence: 93%

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Cluster Analysis of Anther‐derived Plants of Solanum Phureja (Solanaceae) Based on Morphological Characteristics

Pehu

Veilleux

Hilu

1987

American J of Botany

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The feasibility of grouping anther‐derived plants of Solarium phureja according to ploidy based on their morphological characteristics was studied. Canonical discriminant analysis identified four characteristics (anther length, number of chloroplasts per pair of guard cells, leaf width, corolla width at widest diam) of nine measured as the most effective combination for diagnosing ploidy. Data for these characteristics from two sets of plants were subjected to two clustering techniques, one using the average linkage clustering (UPGMA of the NT‐SYS programs) and the other using centroid sorting (SAS‐Fastclus). Screening of anther‐derived plants by cluster analysis proved to be an efficient means of separating monoploids from the other ploidy levels.

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supporting

confidence: 53%

mentioning

confidence: 93%

Cluster Analysis of Anther‐derived Plants of Solanum Phureja (Solanaceae) Based on Morphological Characteristics

Pehu

Veilleux

Hilu

1987

American J of Botany

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“…In general, changes in the morphology of plants caused by polyploidization are related to increased cell size and/ or cell numbers of each individual (Santos and Handro, 1983). Thus, regardless of the particular plant tissue, stomata could be expected to become longer as ploidy level increases.…”

Section: Stomatal Length As a Ploidy Index In Asparagusmentioning

confidence: 99%

Identification of Polyploidy of in vitro Anther-derived Shoots of Asparagus officinalis L. by Flow Cytometric Analysis and Measurement of Stomatal Length

Shiga

Uno

Kanechi

et al. 2009

J. Japan. Soc. Hort. Sci.

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Ploidy levels of in vitro shoots produced through anther culture of asparagus were determined by flow cytometry (FCM) analysis instead of counting the chromosome number of root tips that were difficult to regenerate. Of the 110 anther-derived shoots, there were 83 diploids, 21 tetraploids, 3 octaploids, and 3 mixoploids, but no haploids. When flower buds of different length were used as starting material for anther culture, the richest variation in polyploidy originated from short flower buds (1.0-1.5 mm). This result suggested that polyploids might develop by the fusion of nuclei during pollen-mother cell division before the tetrad stage. We used scanning electron microscopy (SEM) to measure the stomatal length of in vitro and field-grown shoots in order to clarify the differences among polyploids determined by FCM analysis. When the developmental stage of tissue was taken into account, we found statistically significant differences in stomatal length among all polyploids (diploid, tetraploid, and octaploid) for both stems and phyllodes (P < 0.001). Stomata on stems were longer than those on phyllodes, and mature tissue had longer stomata than immature tissue. Morphological observations with SEM found that immature tissue had guard cells set below epidermal cells, and were poorly developed. Field-grown diploids had much shorter stomatal length than in vitro diploids. Male plants grown in the field had significantly longer stomata than female plants in both their immature stems and mature phyllodes (P < 0.001). These results indicated that stomatal length can be a reliable ploidy index by removing any other influential factors such as sex, environment and developmental stage.

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“…Variation in callus color, regeneration capacity, size of the plant, peduncle length, position of spathe compared to leaves, type and number of buds per plant, color of spathe, spadix color, and spadix length was recorded in this study. In other studies, recorded differences in the leaf length:width ratio and petal length in regenerants were derived from anther culture of petunia (Santos and Handro 1983). Different leaf shapes and sizes were reported in anther culture plants of Lilium longiflorum (Qu et al 1988;Han et al 1997).…”

Section: Discussionmentioning

confidence: 91%

Morphological and cytological diversity of regenerants derived from half-anther cultures of anthurium

Winarto¹,

Rachmawati²,

Pramanik³

et al. 2010

Plant Cell Tiss Organ Cult

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Anthurium anther culture was successfully established using half-anthers as explants. Explants were cultured on Winarto-Teixeira basal medium (WT-1) containing 0.01 mg/l a-naphthalene acetic acid (NAA), 0.5 mg/l thidiazuron (TDZ), and 1.0 mg/l 6-benzylaminopurine (BAP), or on New Winarto-Teixeira basal medium (NWT-3) supplemented with 0.02 mg/l NAA, 1.5 mg/l TDZ, and 0.75 mg/l BAP for callus initiation. Regenerated calli produced multiple shoots on WT-1, which were then rooted in NWT-3 supplemented with 1% activated charcoal. Plantlets were acclimatized ex vitro using a mixture of burned rice husk, rice husk, and bamboo peat (1:1:1, v/v/v) as the potting medium. There was considerable morphological and cytological diversity of regenerants derived from anther culture, which are described in detail in this study. The callus cluster color ranged from green to light green and had a high regeneration capacity (7.3 and 4.8 shoots/callus cluster), light reddish-yellow callus showed moderate regeneration (2.6 shoots/callus cluster), while reddish-yellow callus had the lowest regeneration capacity (1.5 shoots/callus cluster). Morphological variations clearly observed in regenerants derived from this technique included alterations in plant size, peduncle length, spathe position compared to leaves, the type and number of buds, spathe and spadix color, and spadix length. There were also cytological variations in both in vitro and ex vitro regenerants of anther culture with 23-29% haploids, 5-10% aneuploids, 56-69% diploids, and 3-4% triploids. The results strengthen other studies in which the development of anther cultures, especially via callus formation, resulted in morphological and cytological alterations. These variations have been discussed to great length in this paper.

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Morphological patterns in Petunia hybrida plants regenerated from tissue cultures and differing by their ploidy

Cited by 14 publications

References 15 publications

Cluster Analysis of Anther‐derived Plants of Solanum Phureja (Solanaceae) Based on Morphological Characteristics

Cluster Analysis of Anther‐derived Plants of Solanum Phureja (Solanaceae) Based on Morphological Characteristics

Identification of Polyploidy of in vitro Anther-derived Shoots of Asparagus officinalis L. by Flow Cytometric Analysis and Measurement of Stomatal Length

Morphological and cytological diversity of regenerants derived from half-anther cultures of anthurium

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