Morphological Studies of the Euterpe oleracea Mart. Seeds

Costa, Roberto Cezar Lobo da; Lobato, Allan Klynger da Silva; Neto, M. A. M.; SantosFilho, B. C.; Maia, W. J. M. S.; Neto, C. F. Oliveira; Barreto, A. G. T.; Lemos, Raimunda Santos de; Gouvea, Dramerson Dorivan Silva

doi:10.3923/ijb.2008.303.308

Cited by 2 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…mature seed. Açaí seed is formed by the seed coat, called testa or tegument, an embryo, and the endosperm 13,19 . The endosperm is majorly composed of mannan ( Figure 1b, x ), a polymer of the hexose mannose, which provides embryo protection, and serves as a reserve for seedling nutrition and development 11,14 .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Acquiring thin histological sections of açaí (Euterpe oleracea Mart.) seeds for molecular mapping by mass spectrometry imaging

Brum

Martins

Mohana‐Borges

et al. 2022

Preprint

View full text Add to dashboard Cite

RATIONALE: Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) of tissues became popular in the last decade. Consequently, adapting sample preparation methods for different materials turned to be a pivotal step for a successful analysis, due to the requirement of samples slices of 12-20 µm thickness. However, preparing thin sections compatible with MALDI-IMS for unusual samples is challenging, as existing histological protocols may not be suitable, thus requiring new methods. Açaí (Euterpe oleracea Mart.) seed is an example of a challenging material due to its toughness and resistance to crack. Therefore, our goal was to develop a methodology to obtain thin (<20 µm) and entire longitudinal sections of açaí seeds for MALDI-IMS analysis. METHODS: Different strategies were evaluated for obtaining thin cuts of seeds, being the combination of the following steps the most suitable option: (i) softening of seeds by water immersion for 24 h; (ii) longitudinal cut of seeds to obtain half-seeds using a razor blade and a hammer; (iii) half-seeds imbibition in gelatin; (iv) sectioning using a cryostat at -20 °C to obtain samples with <20 µm thickness; and (v) collection of samples in an indium tin oxide coated glass slide covered by a double-face copper tape to avoid sample wrapping and ensure adhesion after unfreezing. (iv) storage of samples in a -80 °C freezer, if necessary. RESULTS: As a result, this adapted sample preparation method enabled the analysis of açaí seeds by MALDI-IMS providing spatial distribution of carbohydrates in the endosperm. CONCLUSIONS: The adaptations developed for sample preparation will help investigate the metabolic and physiological properties of açaí seeds in future studies.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Morphologically, the mature açaí seed is enveloped by a fibrous mesocarp and formed by an endocarp that contains a tiny embryo, an abundant endosperm with resistant properties, and a ruminated tegument 4,[13][14][15] . Identifying the location and content of specific compounds across a tissue may help correlate to their function 16 .…”

Section: Introductionmentioning

confidence: 99%

Acquiring thin histological sections of açaí (Euterpe oleracea Mart.) seeds for molecular mapping by mass spectrometry imaging

Brum

Martins

Mohana‐Borges

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

The acquisition of thin sections of açaí (Euterpe oleracea Mart.) seed with elevated potassium content for molecular mapping by mass spectrometry imaging

Brum

Martins

Mohana‐Borges

et al. 2023

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

RationaleMatrix‐assisted laser desorption/ionization imaging mass spectrometry (MALDI‐IMS) of tissues became popular in the last decade. Consequently, adapting sample preparation methods for different materials turned out to be a pivotal step for successful analysis due to the requirement of sample slices of 12–20 μm thickness. However, acquiring thin sections compatible with MALDI‐IMS for unusual samples is challenging, as existing histological protocols may not be suitable, thus requiring new methods. Açaí (Euterpe oleracea Mart.) seed is an example of a challenging material due to its toughness and resistance to crack, therefore our goal was to develop a methodology to obtain thin (12–20 μm) and entire sections of açaí seeds for MALDI‐IMS analysis.MethodsDifferent strategies were evaluated for obtaining thin sections of seeds, and the combination of the following steps was found to be the most suitable option: (i) softening of seeds by water immersion for 24 h; (ii) transversal cut of seeds to obtain half‐seeds using a razor blade and a hammer; (iii) half‐seeds imbibition in gelatin; (iv) samples sectioning using a cryostat at −20°C to obtain samples with 12–20 μm thickness; (v) collection of samples in an indium tin oxide‐coated glass slide covered by double‐sided copper tape to avoid sample wrapping and ensure adhesion after unfreezing; and (vi) storage of samples in a −80°C freezer, if necessary.ResultsThis adapted sample preparation method enabled the analysis of açaí seeds by MALDI‐IMS, providing spatial distribution of carbohydrates in the endosperm.ConclusionsThe adaptations developed for sample preparation will help investigate the metabolic and physiological properties of açaí seeds in future studies.

show abstract

Morphological Studies of the Euterpe oleracea Mart. Seeds

Cited by 2 publications

References 5 publications

Acquiring thin histological sections of açaí (Euterpe oleracea Mart.) seeds for molecular mapping by mass spectrometry imaging

Acquiring thin histological sections of açaí (Euterpe oleracea Mart.) seeds for molecular mapping by mass spectrometry imaging

The acquisition of thin sections of açaí (Euterpe oleracea Mart.) seed with elevated potassium content for molecular mapping by mass spectrometry imaging

Contact Info

Product

Resources

About