Morphological study of the arterial supply to the menisci in pigs with special reference to creating meniscus injury model

Natsuyama, Yutaro; Zhang, Mingshou; Yang, Ting; Shimada, Kazuyuki; Ren, Kaijun; Kamikawa, Yasuko; Chen, Juefei; Yi, Shuang-Qin

doi:10.5603/fm.a2023.0041

Cited by 3 publications

(4 citation statements)

References 24 publications

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“…In adult porcine, we observed the vascular network is limited to a few vessels in the outer region, mostly located on the femoral side. Blood vessels from large arteries enter the meniscus along the tie‐fibers 49 . We observed similarly that blood vessels penetrate the meniscal tissue along or inside the radial tie‐fibers.…”

Section: Discussionsupporting

confidence: 66%

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Age and anatomical region‐related differences in vascularization of the porcine meniscus using microcomputed tomography imaging

Karjalainen,

Herrera Millar,

Modina

et al. 2024

Journal Orthopaedic Research

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Meniscal lesions in vascularized regions are known to regenerate while lack of vascular supply leads to poor healing. Here, we developed and validated a novel methodology for three‐dimensional structural analysis of meniscal vascular structures with high‐resolution microcomputed tomography (µCT). We collected porcine medial menisci from 10 neonatal (not‐developed meniscus, n‐) and 10 adults (fully developed meniscus, a‐). The menisci were cut into anatomical regions (anterior horn (n‐AH and a‐AH), central body (n‐CB and a‐CB), and posterior horn (n‐PH and a‐PH). Specimens were cut in half, fixed, and one specimen underwent critical point drying and µCT imaging, while other specimen underwent immunohistochemistry and vascularity biomarker CD31 staining for validation of µCT. Parameters describing vascular structures were calculated from µCT. The vascular network in neonatal spread throughout meniscus, while in adult was limited to a few vessels in outer region, mostly on femoral side. n‐AH, n‐CB, and n‐PH had 20, 17, and 11 times greater vascular volume fraction than adult, respectively. Moreover, thickness of blood vessels, in three regions, was six times higher in adults than in neonatal. a‐PH appeared to have higher vascular fraction, longer and thicker blood vessels than both a‐AH and a‐CB. Overall, neonatal regions had a higher number of blood vessels, more branching, and higher tortuosity compared to adult regions. For the first time, critical point drying‐based µCT imaging allowed detailed three‐dimensional visualization and quantitative analysis of vascularized meniscal structures. We showed more vascularity in neonatal menisci, while adult menisci had fewer and thicker vascularity especially limited to the femoral surface.

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Section: Discussionsupporting

confidence: 66%

“…Blood vessels from large arteries enter the meniscus along the tie-fibers. 49 We observed similarly that blood vessels penetrate the meniscal tissue along or inside the radial tie-fibers.…”

Section: Cpd-based Sample Drying Protocol Together With High-resoluti...supporting

confidence: 61%

Age and anatomical region‐related differences in vascularization of the porcine meniscus using microcomputed tomography imaging

Karjalainen,

Herrera Millar,

Modina

et al. 2024

Journal Orthopaedic Research

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“…A total of 20 lower limbs of pigs were used in this study, of which four adult pigs were purchased from Nansyu Natural Pork (Kagoshima, Japan) and 16 fetal pig lower limbs were purchased from Bio Corporation (Minnesota, USA). For fetal pigs purchased from the United States, merchants have performed 10% formalin circulation fixation and injected red latex into the arterial system and blue latex into the venous system to identify the blood vessels [2,14,15]. Fetal are suitable for Sihler's staining, while adult pigs are suitable for gross dissection for nerve identification.…”

Section: Preparation Of Specimens and Gross Anatomymentioning

confidence: 99%

“…Masson's trichrome staining was performed according to the methods described in our previous studies [14,15]. Briefly:…”

Section: Histological Evaluationmentioning

confidence: 99%

Comparative study of the peroneus tertius muscle in pigs based on the origin, course, insertion and innervation

Natsuyama,

Yang,

et al. 2024

Folia Morphol

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Vascular anatomy of the lateral meniscus with special focus on the joint capsule

Natsuyama,

Mitsuya,

Kuramasu

et al. 2024

Anat Sci Int

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Previous studies have reported that the lateral meniscus (LM) has two regions, the popliteal hiatus area (PH) with a scarce blood supply and the roots with an abundant one. However, the description of its vascular anatomy remains insufficient. We hypothesized that the difference in the width of the meniscus hilum (MH) affects the scarcity and abundance of blood supply to the LM. The MH is a concept proposed by us and is the only site of entrance or exit of blood vessels and nerves associated with the meniscus. The purpose of this study was to provide a structural explanation for the disparity of blood supply to the LM using the concept of MH. Sixteen knees were examined to investigate the blood supply to LM. In most areas, the femoral joint capsule (FJC) and tibial joint capsule (TJC) continued to the cranial and caudal edges of the LM, respectively. In the roots, the FJC and TJC covered the femoral and the outer-femoral surfaces. In contrast, the FJC in the PH did not attach to the cranial edge and only the TJC there did to the caudal edge of the LM. Histochemical examination showed that the blood vessels enter the LM via the MH. In the PH, the MH at the caudal edge was extremely narrow; and in the roots, the MH on the outer-femoral surfaces was wide. The results suggest that the difference in the width of the MH affected the scarcity and abundance of blood supply to the LM.

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Morphological study of the arterial supply to the menisci in pigs with special reference to creating meniscus injury model

Abstract: This article has been peer reviewed and published immediately upon acceptance.It is an open access article, which means that it can be downloaded, printed, and distributed freely, provided the work is properly cited. Articles in "Folia Morphologica" are listed in PubMed.

Cited by 3 publications

References 24 publications

Age and anatomical region‐related differences in vascularization of the porcine meniscus using microcomputed tomography imaging

Age and anatomical region‐related differences in vascularization of the porcine meniscus using microcomputed tomography imaging

Comparative study of the peroneus tertius muscle in pigs based on the origin, course, insertion and innervation

Vascular anatomy of the lateral meniscus with special focus on the joint capsule

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