Morphological Study on the Gastric Mucosa in Diabetes Mellitus Rats Induced by Streptozotocin

Watanabe, Takao; Asanuma, Akimune; Tanaka, Masahiro; Akiba, Tomohide; Koga, Teruji

doi:10.1538/expanim1978.43.5_693

Cited by 4 publications

(2 citation statements)

References 4 publications

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“…Although the underlying biochemical mechanism(s) for the prevalence of spontaneous mucosal injury in diabetic animals remains to be fully elucidated, data from earlier studies suggest that decreased thickness of the surface epithelium could be a contributing factor. Watanabe et al (23) reported a decreased surface epithelial cell layer in the gastric mucosa of diabetic rats. In the current investigation, we have also noted the mucosal thickness to be comparatively lower in overnight-fasted STZ-diabetic rats than in their healthy counterparts.…”

Section: Discussionmentioning

confidence: 97%

Induction of EGFR Tyrosine Kinase in the Gastric Mucosa of Diabetic Rats

Khan¹,

Fligiel²,

Liu³

et al. 1999

Experimental Biology and Medicine

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Diabetes mellitus is associated with spontaneous gastric mucosal injury and enhanced susceptibility of the mucosa to damaging agents. Little information is available about the biochemical changes that occur in the gastric mucosa of diabetes mellitus. Evidence is accumulating that tyrosine kinases, particularly the EGFreceptor (EGFR), are involved in regulating a variety of structural and functional properties of the gastric mucosa. The primary objectives of this investigation were to determine whether diabetes induces morphological changes in the gastric mucosa, and if so, whether these changes are associated with alterations in EGFR tyrosine kinase. Diabetes-induced changes in gastric mucosal morphology were also examined. Diabetes was induced in 3-to 4-month-old male Fischer-344 rats by streptozotocin (STZ; 45 mg/kg; i.v.). Four weeks after induction of diabetes mellitus, the gastric mucosa of overnight-fasted rats was found to be slightly atrophic. A reduction in gastric mucosal thickness with deposition of fibrous tissue above the muscularis layer was observed in the stomach of overnight-fasted diabetic rats. These changes were associated with a marked stimulation in tyrosine kinase activity and protein expression of EGFR. The relative concentrations of several precursor forms of TGF-a in both membrane and cytosolic fractions from the gastric mucosa of overnight-fasted diabetic rats were also found to be significantly above the corresponding controls. This suggests that endogenous TGF-CY may play a critical role in regulating mucosal EGFR tyrosine kinase through a juxtacrine/paracrine mechanism.

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Section: Discussionmentioning

confidence: 97%

Induction of EGFR Tyrosine Kinase in the Gastric Mucosa of Diabetic Rats

Khan¹,

Fligiel²,

Liu³

et al. 1999

Experimental Biology and Medicine

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“…The present results clearly showed that gastric emptying was remarkably lower in STZ-induced diabetic rats. Furthermore, we also found that the thickness of gastric mucosa prominently increased in diabetic rats [ 13 ]. Delayed gastric emptying is now recognized as part of a broader spectrum of gastric neuromuscular dysfunction that includes impaired gastric accommodation [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Increased expression of hypoxia inducible factor-1 alpha and vascular endothelial growth factor is associated with diabetic gastroparesis

Zhong

et al. 2020

BMC Gastroenterol

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Background Gastroparesis is a recognized complication of diabetes but its pathogenic mechanism incompletely understood. Our aim was to determine whether HIF-1α and VEGF are secreted from gastric tissue is a fundamental factor that drives diabetic gastroparesis. Methods Diabetes was induced in Sprague-Dawley by a single intraperitoneal injection of 65 mg/kg streptozotocin. After 4 and 12 weeks, rats were euthanized for assaying body weight, blood glucose, gastric acid secretion and gastric emptying. Morphologic changes in gastric mucosa were observed by the light microscope. Expression of HIF-1α and VEGF were assessed using immunohistochemistry, RT-PCR and Western blot analyses. Results Compared with control group, blood glucose were significantly increased and body weight were markedly decreased in streptozotocin-induced diabetes. Gastric emptying was significantly decreased in diabetic rats compared to the control group at different times. The number of parietal cells was obviously decreased, and vacuolated degeneration in diabetic rats. Gastric acid secretion in diabetic group was significantly decreased, and expression of HIF-1α and VEGF were significantly increased in the diabetic group. Conclusion These results indicated that overexpression of HIF-1α and VEGF in the gastric mucosa and played a pivotal role in the progression of diabetic gastroparesis.

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Ravages of Diabetes on Gastrointestinal Sensory-Motor Function: Implications for Pathophysiology and Treatment

Gregersen

Liao

Drewes

et al. 2016

Curr Gastroenterol Rep

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Symptoms related to functional and sensory abnormalities are frequently encountered in patients with diabetes mellitus. Most symptoms are associated with impaired gastric and intestinal function. In this review, we discuss basic concepts of sensory-motor dysfunction and how they relate to clinical findings and gastrointestinal abnormalities that are commonly seen in diabetes. In addition, we review techniques that are available for investigating the autonomic nervous system, neuroimaging and neurophysiology of sensory-motor function. Such technological advances, while not readily available in the clinical setting, may facilitate stratification and individualization of therapy in diabetic patients in the future. Unraveling the structural, mechanical, and sensory remodeling in diabetes disease is based on a multidisciplinary approach that can bridge the knowledge from a variety of scientific disciplines. The final goal is to increase the understanding of the damage to GI structures and to sensory processing of symptoms, in order to assist clinicians with developing an optimal mechanics based treatment.

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Morphological Study on the Gastric Mucosa in Diabetes Mellitus Rats Induced by Streptozotocin

Cited by 4 publications

References 4 publications

Induction of EGFR Tyrosine Kinase in the Gastric Mucosa of Diabetic Rats

Induction of EGFR Tyrosine Kinase in the Gastric Mucosa of Diabetic Rats

Increased expression of hypoxia inducible factor-1 alpha and vascular endothelial growth factor is associated with diabetic gastroparesis

Ravages of Diabetes on Gastrointestinal Sensory-Motor Function: Implications for Pathophysiology and Treatment

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