2008
DOI: 10.1529/biophysj.108.132548
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Morphological Transitions of Vesicles Induced by Alternating Electric Fields

Abstract: When subjected to alternating electric fields in the frequency range 10(2)-10(8) Hz, giant lipid vesicles attain oblate, prolate, and spherical shapes and undergo morphological transitions between these shapes as one varies the field frequency and/or the conductivities lambda(in) and lambda(ex) of the aqueous solution inside and outside the vesicles. Four different transitions are observed with characteristic frequencies that depend primarily on the conductivity ratio lambda(in)/lambda(ex). The theoretical mod… Show more

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Cited by 102 publications
(147 citation statements)
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“…The prolate semi-axes a and b are shown. The GUV contained 0.2 mM NaCl inside to ensure that the conductivity inside the vesicle was higher than outside, and therefore the AC field would cause a prolate deformation (Aranda et al 2008). The table shows the maximum area increase ΔA/A o (measured directly from a/b for 20-50 vesicles/system), the TX-100 to lipid molar ratio at the saturating condition R e sat and the binding constant K. The details are discussed in Mattei et al (2015).…”
Section: Solubilization Of Binary Lipid Mixtures Containing Cholesterolmentioning
confidence: 99%
See 1 more Smart Citation
“…The prolate semi-axes a and b are shown. The GUV contained 0.2 mM NaCl inside to ensure that the conductivity inside the vesicle was higher than outside, and therefore the AC field would cause a prolate deformation (Aranda et al 2008). The table shows the maximum area increase ΔA/A o (measured directly from a/b for 20-50 vesicles/system), the TX-100 to lipid molar ratio at the saturating condition R e sat and the binding constant K. The details are discussed in Mattei et al (2015).…”
Section: Solubilization Of Binary Lipid Mixtures Containing Cholesterolmentioning
confidence: 99%
“…To that purpose, a different experimental setup was used. GUVs were placed in a special chamber with two electrodes and addition of TX-100 was carried out in the presence of an AC field (Mattei et al 2015), which is known to induce prolate deformation in GUVs with excess area (Aranda et al 2008;Riske et al 2009). The extent of deformation, quantified though the ratio between the two semi-axes a/b (see Fig.…”
Section: Solubilization Of Binary Lipid Mixtures Containing Cholesterolmentioning
confidence: 99%
“…Electrofusion-based reactions in GUVs strongly rely on the physical behavior (deformation, poration and fusion) of these vesicles subjected to either alternating current field or strong direct current (DC) pulses. In recent years, our group has systematically investigated this topic by experimental and theoretical modeling (Riske et al, 2005;Riske et al, 2006;Haluska et al, 2006;Dimova et al, 2007;Aranda et al, 2008;Riske et al, 2009;Vlahovska et al, 2009;Dimova et al, 2009;Yamamoto et al, 2010). Based on the findings from our and other research groups, successful electrofusion is b a s e d o n f o u r i m p o r t a n t f e a t u r e s o f t h e membrane and the vesicles: 1) The lipid membrane is impermeable to reactants (ions and macromolecules), while water can freely permeate through the membrane to assure osmotic balance; 2) Fast and effective fusion could be initiated with a short electric pulse with characteristics above the poration threshold of the membrane; 3) Leakage of reactants during the fusion process is negligible (Riske et al, 2005); 4) Vesicles with complementary reactants can be brought together to form a reactive couple for electrofusion.…”
Section: Wwwintechopencommentioning
confidence: 99%
“…Objetiva 10x e barra de escala referente a 50 µm. GUVs quando submetidas ao campo elétrico alternado podem obter as formas prolata, oblata e esférica, dependendo da razão entre as condutividades interna (λ in ) e externa (λ ex ) da solução (Aranda et al, 2008). Neste estudo, investigou-se a interação da AnxA5 com GUVs, sob ação de um campo elétrico alternado com amplitude constante, com o objetivo de confirmar a reconstituição funcional da AnxA5 quando esta interage com modelos de membranas.…”
Section: Interação Da Anxa5 Com a Membrana De Guvsunclassified
“…É importante ressaltar que não foi observado perda de contraste óptico durante os experimentos de aplicação de campo elétrico, indicando a formação de canais Ca-seletivos na membrana (Aranda et al 2008).…”
Section: Interação Da Anxa5 Com a Membrana De Guvsunclassified