Morphology and cellular-traction of fibroblasts on 2D silk-fibroin hydrogel substrates

Edwin, Privita Edwina Rayappan George; Rajagopalan, Neeraj Raghuraman; Bajpai, Saumendra

doi:10.1080/1539445x.2021.1918719

Cited by 2 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, NIH3T3 fibroblast cells were cultured on the surface of corresponding hydrogel. It has been reported that the increase of hydrogel stiffness decreased nucleus-to-cytoplasm area-ratio and increased asymmetricity along the major-axis of cells [ 22 ]. Moreover, using traction force microscopy (TFM), they have carefully quantified corresponding cell traction stress on the hydrogel surface.…”

Section: Advanced Biomedical Applications Of Rsf Materialsmentioning

confidence: 99%

“…Majority of the RSF materials could be used for the basic study of cell-material interactions because of the good cytocompatibility and its convenient for various physical and chemical modifications [ 17 , 18 ]. Such as revealing the effects of macro or nano topological features [ 19 ], fiber arrangements [ 20 ], pore sizes [ 21 ], mechanical properties [ 22 ], and even material degradation process [ 23 , 24 ] on cell behaviors. Among these functional reconstructed biomaterials, RSF films are of particular interest for bioelectronic devices because of the transparency (over 90% transmission across the visible range) and flexibility [ [25] , [26] , [27] ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bioinspired silk fibroin materials: From silk building blocks extraction and reconstruction to advanced biomedical applications

Yao

Zou

Fan

et al. 2022

Materials Today Bio

View full text Add to dashboard Cite

Section: Advanced Biomedical Applications Of Rsf Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioinspired silk fibroin materials: From silk building blocks extraction and reconstruction to advanced biomedical applications

Yao

Zou

Fan

et al. 2022

Materials Today Bio

View full text Add to dashboard Cite

Trends in mechanobiology guided tissue engineering and tools to study cell-substrate interactions: a brief review

et al. 2023

View full text Add to dashboard Cite

Sensing the mechanical properties of the substrates or the matrix by the cells and the tissues, the subsequent downstream responses at the cellular, nuclear and epigenetic levels and the outcomes are beginning to get unraveled more recently. There have been various instances where researchers have established the underlying connection between the cellular mechanosignalling pathways and cellular physiology, cellular differentiation, and also tissue pathology. It has been now accepted that mechanosignalling, alone or in combination with classical pathways, could play a significant role in fate determination, development, and organization of cells and tissues. Furthermore, as mechanobiology is gaining traction, so do the various techniques to ponder and gain insights into the still unraveled pathways. This review would briefly discuss some of the interesting works wherein it has been shown that specific alteration of the mechanical properties of the substrates would lead to fate determination of stem cells into various differentiated cells such as osteoblasts, adipocytes, tenocytes, cardiomyocytes, and neurons, and how these properties are being utilized for the development of organoids. This review would also cover various techniques that have been developed and employed to explore the effects of mechanosignalling, including imaging of mechanosensing proteins, atomic force microscopy (AFM), quartz crystal microbalance with dissipation measurements (QCMD), traction force microscopy (TFM), microdevice arrays, Spatio-temporal image analysis, optical tweezer force measurements, mechanoscanning ion conductance microscopy (mSICM), acoustofluidic interferometric device (AID) and so forth. This review would provide insights to the researchers who work on exploiting various mechanical properties of substrates to control the cellular and tissue functions for tissue engineering and regenerative applications, and also will shed light on the advancements of various techniques that could be utilized to unravel the unknown in the field of cellular mechanobiology. Graphical Abstract

show abstract

Morphology and cellular-traction of fibroblasts on 2D silk-fibroin hydrogel substrates

Cited by 2 publications

References 40 publications

Bioinspired silk fibroin materials: From silk building blocks extraction and reconstruction to advanced biomedical applications

Bioinspired silk fibroin materials: From silk building blocks extraction and reconstruction to advanced biomedical applications

Trends in mechanobiology guided tissue engineering and tools to study cell-substrate interactions: a brief review

Contact Info

Product

Resources

About