Morphology and cross-linkage of stereocilia in the guinea-pig labyrinth examined without the use of osmium as a fixative

Osborne, Michael P.; Comis, S. D.; Pickles, James O.

doi:10.1007/bf00229198

Cited by 74 publications

(33 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tissues were processed by using the OTOTO method (Osborne et al, 1984). Samples were dehydrated in increasing concentrations of ethanol and critical point dried with CO 2 in a SamDri 790 (Tousmis, Rockville, MD).…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Defects in vestibular sensory epithelia and innervation in mice with loss of Chd7 function: Implications for human CHARGE syndrome

Adams

Hurd

Beyer

et al. 2007

J of Comparative Neurology

View full text Add to dashboard Cite

CHD7 is a chromodomain gene mutated in CHARGE syndrome, a multiple anomaly condition characterized by ocular coloboma, heart defects, atresia of the choanae, retarded growth and development, genital hypoplasia, and ear defects including deafness and semicircular canal dysgenesis. Mice with heterozygous Chd7 deficiency have circling behavior and semicircular canal defects and are an excellent animal model for exploring the pathogenesis of CHARGE features. Inner ear vestibular defects have been characterized in heterozygous Chd7-deficient embryos and early postnatal mice, but it is not known whether vestibular defects persist throughout adulthood in Chd7-deficient mice or whether the vestibular sensory epithelia and their associated innervation and function are intact. Here we describe a detailed analysis of inner ear vestibular structures in mature mice that are heterozygous for a Chd7-deficient, gene-trapped allele (Chd7(Gt/+)). Chd7(Gt/+) mice display variable asymmetric lateral and posterior semicircular canal malformations, as well as defects in vestibular sensory epithelial innervation despite the presence of intact hair cells in the target organs. These observations have important functional implications for understanding the clinical manifestations of CHD7 mutations in humans and for designing therapies to treat inner ear vestibular dysfunction.

show abstract

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Defects in vestibular sensory epithelia and innervation in mice with loss of Chd7 function: Implications for human CHARGE syndrome

Adams

Hurd

Beyer

et al. 2007

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…Ultrathin sections were placed on copper grids and analyzed in a Philips (Aachen, Germany) 208 electron microscope. For scanning electron microscopy (SEM), inner ear sensory epithelia were fixed with 2.5% glutaraldehyde, 0.05 M sucrose, and 1% tannic acid in 0.05 M N, N-bis[2-hydroxyethyl]2-aminoethanesulfonic acid buffer, pH 7.4, for 3 weeks at ϳ4°C as described previously (Osborne et al, 1984). The samples were dehydrated with an ethanol series (50, 70, 80, 90, and 100%), critical-point dried from liquid CO 2 , sputter coated with gold-palladium, and examined using a scanning electron micro- Figure 1.…”

Section: Animals and Antibodies Ames Waltzer C57bl/ 6j-pcdh15mentioning

confidence: 99%

Physical and Functional Interaction between Protocadherin 15 and Myosin VIIa in Mechanosensory Hair Cells

Senften¹,

Schwander²,

et al. 2006

View full text Add to dashboard Cite

Hair cells of the mammalian inner ear are the mechanoreceptors that convert sound-induced vibrations into electrical signals. The molecular mechanisms that regulate the development and function of the mechanically sensitive organelle of hair cells, the hair bundle, are poorly defined. We link here two gene products that have been associated with deafness and hair bundle defects, protocadherin 15 (PCDH15) and myosin VIIa (MYO7A), into a common pathway. We show that PCDH15 binds to MYO7A and that both proteins are expressed in an overlapping pattern in hair bundles. PCDH15 localization is perturbed in MYO7A-deficient mice, whereas MYO7A localization is perturbed in PCDH15-deficient mice. Like MYO7A, PCDH15 is critical for the development of hair bundles in cochlear and vestibular hair cells, controlling hair bundle morphogenesis and polarity. Cochlear and vestibular hair cells from PCDH15-deficient mice also show defects in mechanotransduction. Together, our findings suggest that PCDH15 and MYO7A cooperate to regulate the development and function of the mechanically sensitive hair bundle.

show abstract

“…Cuprolinic blue treatment also revealed electron-dense anionic regions at both ends of the tip link that may serve to anchor this link to the stereocilium membrane (Tsuprun and Santi 1998). The second set of stereocilia links is the side links, which run laterally between and across the rows of the stereocilia and connect the stereocilia to one another to maintain the morphological integrity of the bundle Osborne et al 1984;Furness and Hackney 1985). A model for the mechanics of the stereocilia bundle (Pickles 1993) predicts that the tip links are in an appropriate position to detect stereocilia deflections, whereas the main role for the side links may be to couple the deflections among the stereocilia.…”

mentioning

confidence: 99%

Structure of Outer Hair Cell Stereocilia Side and Attachment Links in the Chinchilla Cochlea

Tsuprun

Santi

2002

J Histochem Cytochem.

View full text Add to dashboard Cite

S U M M A R YThe structure and symmetry of chinchilla outer hair cell (OHC) stereocilia side and attachment links were investigated by transmission electron microscopy using tannic acid and Cuprolinic blue histochemical procedures. The side links run laterally between and across the rows of the stereocilia and connect the stereocilia together within the bundle. Attachment links form a crown-like array around the tips of only the tallest OHC stereocilia and attach these stereocilia to the Type B fibrils of the tectorial membrane. Computer averaging of the side links from tannic acid-treated tissues showed a central dense region of the link between adjacent stereocilia and a smaller dense portion at the plasma membrane end of the link. Computer averaging of Cuprolinic blue-treated tissues showed low electron density of the central region of the link, and the plasma membrane ends of the link were electron dense. After tannic acid treatment, the attachment links showed a diffused radial distribution around the tips of the tallest OHC stereocilia. After Cuprolinic blue treatment, the attachment links appeared as electron-dense, membrane-bound granular structures arranged with radial symmetry. The central regions of the side links are reactive to tannic acid. These regions appear to contain neutral and basic residues of proteins and participate in side-by-side association of the side links in regular aggregates. Cuprolinic blue-reactive regions of the side and attachment links appear to contain acidic sulfated residues of glycoproteins or proteoglycans, which may be involved in the attachment of these links to the stereocilium membrane.

show abstract

Morphology and cross-linkage of stereocilia in the guinea-pig labyrinth examined without the use of osmium as a fixative

Cited by 74 publications

References 19 publications

Defects in vestibular sensory epithelia and innervation in mice with loss of Chd7 function: Implications for human CHARGE syndrome

Defects in vestibular sensory epithelia and innervation in mice with loss of Chd7 function: Implications for human CHARGE syndrome

Physical and Functional Interaction between Protocadherin 15 and Myosin VIIa in Mechanosensory Hair Cells

Structure of Outer Hair Cell Stereocilia Side and Attachment Links in the Chinchilla Cochlea

Contact Info

Product

Resources

About