Morphology and Distribution of Tanycytes in the Third Ventricle of the Adult Rat

Mathew, T.C.; Singh, Dara

doi:10.1159/000146709

Cited by 9 publications

(5 citation statements)

References 2 publications

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“…Serotonergic fibers project directly into the cerebrospinal fluid (Moore, 1977;Takeuchi and Sano, 1983) and serotonin acts within the cerebrospinal fluid to increase ciliary beat frequency (Nguyen et al, 2001). Ciliated ependymal cells are widely distributed throughout the ventricular system (Mathew and Singh, 1989;Mathew, 1999;Mathew, 2008). This may serve to increase clearance of bioactive molecules and metabolites following stress-related stimuli, and therefore may contribute to the stress coping functions that have been attributed to Ucn 2, Ucn 3 and CRF 2 receptor activation (Bale et al, 2000;Coste et al, 2000;Hsu and Hsueh, 2001).…”

Section: Discussionmentioning

confidence: 99%

Urocortin 2 increases c-Fos expression in serotonergic neurons projecting to the ventricular/periventricular system

Hale

Stamper

Staub

et al. 2010

Experimental Neurology

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Serotonin plays an important role in the regulation of anxiety states and physiological responses to aversive stimuli. Intracerebroventricular (i.c.v.) injection of the stress- and anxiety-related neuropeptide urocortin 2 (Ucn 2) increases c-Fos expression in serotonergic neurons in the dorsal (DRD) and caudal (DRC) parts of the dorsal raphe nucleus. These regions contain a subset of serotonergic neurons that projects via the dorsal raphe periventricular tract to periventricular structures, including the subfornical organ and ependymal layer, and to the ventricular system. To determine if Ucn 2 activates ventricle/periventricular-projecting serotonergic neurons in the midbrain raphe complex we made i.c.v. injections of the retrograde tracer Fluoro-Gold into the lateral ventricle, followed 7 days later by i.c.v. injection of Ucn 2. The DRD at −8.18 mm and the DRC at −8.54 mm and −9.16 mm bregma were analyzed using a combined brightfield and immunofluorescence technique. Approximately 40% of the ventricle/periventricular-projecting neurons in the subdivisions sampled were serotonergic. Urocortin 2 increased c-Fos expression in ventricle/periventricular-projecting serotonergic neurons in the DRC and in non-ventricle/periventricular-projecting serotonergic neurons in the DRD and DRC. Of the total population of ventricle/periventricular-projecting serotonergic neurons in the DRC at −8.54 and −9.16 mm bregma, 35% expressed c-Fos following Ucn 2 injections. These data are consistent with previous studies showing that i.c.v. injection of Ucn 2 activates subpopulations of serotonergic neurons restricted to the mid-rostrocaudal DRD and DRC, and further demonstrate that these include both subsets of serotonergic neurons that do and do not project to the ventricle/periventricular system.

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Section: Discussionmentioning

confidence: 99%

Urocortin 2 increases c-Fos expression in serotonergic neurons projecting to the ventricular/periventricular system

Hale

Stamper

Staub

et al. 2010

Experimental Neurology

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“…20 Tanycytes are bipolar specialized ependymal cells located predominantly in the ependymal lining of the floor and ventrolateral walls of the third ventricle. 78 The ventricular surface of tanycytes is in general devoid of kinocilia (Fig. 24).…”

Section: Unusual Cell Typesmentioning

confidence: 99%

Nonlesions, Unusual Cell Types, and Postmortem Artifacts in the Central Nervous System of Domestic Animals

Wohlsein¹,

Deschl

Baumgärtner³

2012

Vet Pathol

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In the central nervous system (CNS) of domestic animals, numerous specialized normal structures, unusual cell types, findings of uncertain or no significance, artifacts, and various postmortem alterations can be observed. They may cause confusion for inexperienced pathologists and those not specialized in neuropathology, leading to misinterpretations and wrong diagnoses. Alternatively, changes may mask underlying neuropathological processes. ''Specialized structures'' comprising the hippocampus and the circumventricular organs, including the vascular organ of the lamina terminalis, subfornical organ, subcommissural organ, pineal gland, median eminence/neurohypophyseal complex, choroid plexus, and area postrema, are displayed. Unusual cell types, including cerebellar external germinal cells, CNS progenitor cells, and Kolmer cells, are presented. In addition, some newly recognized cell types as of yet incompletely understood significance and functionality, such as synantocytes and aldynoglia, are introduced and described. Unusual reactive astrocytes in cats, central chromatolysis, neuronal vacuolation, spheroids, spongiosis, satellitosis, melanosis, neuromelanin, lipofuscin, polyglucosan bodies, and psammoma bodies may represent incidental findings of uncertain or no significance and should not be confused with significant microscopic changes. Auto-and heterolysis as well as handling and histotechnological processing may cause postmortem morphological changes of the CNS, including vacuolization, cerebellar conglutination, dark neurons, Buscaino bodies, freezing, and shrinkage artifacts, all of which have to be differentiated from genuine lesions. Postmortem invasion of microorganisms should not be confused with intravital infections. Awareness of these different changes and their recognition are a prerequisite for identifying genuine lesions and may help to formulate a professional morphological and etiological diagnosis.

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“…3A and 3G). No expression of RFP-MT 1 was found in the ventral wall of the third ventricle where the tanycytes are localized (Bruni 1998; Mathew and Singh 1989), or in the dorsal area. However, using immunohistochemistry, we detected cells expressing the RFP- MT 1 in the ependyma lining the third ventricle dorsal wall (Fig.…”

Section: Resultsmentioning

confidence: 97%

Distribution of MT₁ Melatonin Receptor Promoter-Driven RFP Expression in the Brains of BAC C3H/HeN Transgenic Mice

Adamah-Biassi

Zhang

Jung

et al. 2013

J Histochem Cytochem.

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The pineal hormone melatonin activates two G-protein coupled receptors (MT1 and MT2) to regulate in part biological functions. The MT1 and MT2 melatonin receptors are heterogeneously distributed in the mammalian brain including humans. In the mouse, only a few reports have assessed the expression of the MT1 melatonin receptor expression using 2-iodomelatonin binding, in situ hybridization and/or polymerase chain reaction (PCR). Here, we described a transgenic mouse in which red fluorescence protein (RFP) is expressed under the control of the endogenous MT1 promoter, by inserting RFP cDNA at the start codon of MTNR1a gene within a bacterial artificial chromosome (BAC) and expressing this construct as a transgene. The expression of RFP in the brain of this mouse was examined either directly under a fluorescent microscope or immunohistochemically using an antibody against RFP (RFP-MT1). RFP-MT1 expression was observed in many brain regions including the subcommissural organ, parts of the ependyma lining the lateral and third ventricles, the aqueduct, the hippocampus, the cerebellum, the pars tuberalis, the habenula and the habenula commissure. This RFP-MT1 transgenic model provides a unique tool for studying the distribution of the MT1 receptor in the brain of mice, its cell-specific expression and its function in vivo.

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Morphology and Distribution of Tanycytes in the Third Ventricle of the Adult Rat

Cited by 9 publications

References 2 publications

Urocortin 2 increases c-Fos expression in serotonergic neurons projecting to the ventricular/periventricular system

Urocortin 2 increases c-Fos expression in serotonergic neurons projecting to the ventricular/periventricular system

Nonlesions, Unusual Cell Types, and Postmortem Artifacts in the Central Nervous System of Domestic Animals

Distribution of MT₁ Melatonin Receptor Promoter-Driven RFP Expression in the Brains of BAC C3H/HeN Transgenic Mice

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Morphology and Distribution of Tanycytes in the Third Ventricle of the Adult Rat

Cited by 9 publications

References 2 publications

Urocortin 2 increases c-Fos expression in serotonergic neurons projecting to the ventricular/periventricular system

Urocortin 2 increases c-Fos expression in serotonergic neurons projecting to the ventricular/periventricular system

Nonlesions, Unusual Cell Types, and Postmortem Artifacts in the Central Nervous System of Domestic Animals

Distribution of MT1 Melatonin Receptor Promoter-Driven RFP Expression in the Brains of BAC C3H/HeN Transgenic Mice

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Distribution of MT₁ Melatonin Receptor Promoter-Driven RFP Expression in the Brains of BAC C3H/HeN Transgenic Mice