Morphology and LPS content for the estimation of marine bacterioplankton biomass in the Ionian Sea

Ferla, Rosabruna La; Giudice, Angelina Lo; Maimone, Giovanna

doi:10.3989/scimar.2004.68n123

Cited by 27 publications

(18 citation statements)

References 41 publications

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“…Bacterioplankton (BA) abundance was determined using DAPI (Porter and Porter & Feig 1980) and image analysis (Zeiss AXIOPLAN 2 Imaging microscope). Bacterioplankton biomass (BB) was estimated by cell counting and volumetric measurements according to La Ferla et al. (2004).…”

Section: Methodsmentioning

confidence: 99%

A multidisciplinary study of the Cape Peloro brackish area (Messina, Italy): characterisation of trophic conditions, microbial abundances and activities

Leonardi

Azzaro

et al. 2009

Marine Ecology

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In the framework of the VECTOR DIVCOST Project, a 2‐year investigation was started in 2006, with the aim of testing the sensibility of microbial parameters to environmental changes and of assessing whether they can provide information about functional changes in the carbon cycle. The investigation was performed in the surface waters of two small brackish ponds (Ganzirri and Faro), located in the Cape Peloro transitional area (Sicily, Italy). The seasonal changes in both the microbial compartment [bacterioplankton, vibrios, exoenzymatic hydrolysis of proteins and polysaccharides, bacterial secondary production (HBP) and community respiration] and the trophic state of suspended matter [total suspended matter (TSM), particulate organic carbon (POC), particulate organic nitrogen (PON), C/N] were analysed in relation to the hydrological characteristics [temperature, salinity, oxygen, fluorescence, NH4, NO2, NO3, PO4]. Despite marked differences in the nutritional input and the diversification in both carbon budget and trophic level, the two ponds show similar trends in many of the investigated factors, hardly influenced by seasonal variations. Temporally coupled trends were observed for some parameters (enzyme activities, vibrios abundances, respiratory activity), whereas others (POC, PON, heterotrophic bacterial production, bacterioplankton) showed a seasonal shift between the two lakes. The different behaviour found for the some biotic parameters suggests that their response to environmental conditions may be modulated differently between the two lakes, which, despite their spatial proximity and reciprocal connection, do not always show contemporaneous functional processes.

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Section: Methodsmentioning

confidence: 99%

A multidisciplinary study of the Cape Peloro brackish area (Messina, Italy): characterisation of trophic conditions, microbial abundances and activities

Leonardi

Azzaro

et al. 2009

Marine Ecology

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“…Prokaryotic biomass (PB) was estimated from cell counts and volumetric measurements according to [20].…”

Section: Analytical Determinationsmentioning

confidence: 99%

Enzymatic Activities and Prokaryotic Abundance in Relation to Organic Matter along a West–East Mediterranean Transect (TRANSMED Cruise)

et al. 2012

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The distribution of extracellular enzymatic activities (EEA) [leucine aminopeptidase (LAP), ß-glucosidase (GLU), alkaline phosphatase (AP)], as well as that of prokaryotic abundance (PA) and biomass (PB), dissolved organic carbon (DOC), particulate organic carbon and particulate total nitrogen (POC, PTN), was determined in the epi-, meso-, and bathypelagic waters of the Mediterranean Sea along a West-East transect and at one Atlantic station located outside the Strait of Gibraltar. This study represents a synoptical evaluation of the microbial metabolism during early summer. Decreasing trends with depth were observed for most of the parameters (PA, PB, AP, DOC, POC, PTN). Significant differences between the western and eastern basins of the Mediterranean Sea were found, displaying higher rates of LAP and GLU and lower C/N ratios more in the eastern than in the western areas. Conversely, in the epipelagic layer, PA and PB were found to be higher in the western than in the eastern basins. PB was significantly related to DOC concentration (all data, n = 145, r = 0.53, P < 0.01), while significant correlations of EEA with POC and PTN were found in the epipelagic layer, indicating an active response of microbial metabolism to organic substrates. Specific enzyme activities normalized to cell abundance pointed out high values of LAP and GLU in the bathypelagic layer, especially in the eastern basin, while cell-specific AP was high in the epi- and bathypelagic zone of the eastern basin indicating a rapid regeneration of inorganic P for both prokaryotes and phytoplankton needs. Low activity and abundance characterized the Atlantic station, while opposite trends of these parameters were observed along the Mediterranean transect, showing the uncoupling between abundance and activity data. In the east Mediterranean Sea, decomposition processes increased probably in response to mesoscale structures which lead to organic matter downwelling.

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“…More than 300 cells per sample were counted in randomly selected eye fields. Counts were determined by epifluorescence microscopy as previously described (La Ferla et al, 2004).…”

Section: Total Countsmentioning

confidence: 99%

Predominance ofFlavobacterium,Pseudomonas, andPolaromonaswithin the prokaryotic community of freshwater shallow lakes in the northern Victoria Land, East Antarctica

Michaud

Caruso

Mangano

et al. 2012

FEMS Microbiol Ecol

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A polyphasic approach that included PCR-dependent and PCR-independent molecular techniques was applied to analyze the prokaryotic community in surface waters of shallow Antarctic lakes. The in situ abundance of different bacterial groups was determined by the fluorescence in situ hybridization, whereas bacterial diversity was investigated by 16S rRNA gene sequencing of bacterial clones and isolates. The different approaches allowed the identification of the significant microbial components of the lake bacterioplanktonic communities, indicating a predominance of Flavobacterium, Pseudomonas, and Polaromonas (up to about 56% of total sequences). These genera also appear to be important in freshwater systems elsewhere in the world. Interestingly, closest blast matches to our sequences were predominantly from polar lakes and ponds, in addition to streams and glaciers, suggesting a bipolar distribution of freshwater lake bacterioplankton. Bacteria that are more traditionally associated with the marine environment were also detected, thus indicating an external input by atmospheric deposition and/or seabird excreta. Finally, a slightly different microbial community occurred in the lake at Inexpressible Island that was characterized by low N : P ratio and very high conductivity value, reinforcing the idea that physicochemical and trophic status may affect the structure and composition of the bacterioplankton assemblages in Antarctic lakes.

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Morphology and LPS content for the estimation of marine bacterioplankton biomass in the Ionian Sea

Cited by 27 publications

References 41 publications

A multidisciplinary study of the Cape Peloro brackish area (Messina, Italy): characterisation of trophic conditions, microbial abundances and activities

A multidisciplinary study of the Cape Peloro brackish area (Messina, Italy): characterisation of trophic conditions, microbial abundances and activities

Enzymatic Activities and Prokaryotic Abundance in Relation to Organic Matter along a West–East Mediterranean Transect (TRANSMED Cruise)

Predominance ofFlavobacterium,Pseudomonas, andPolaromonaswithin the prokaryotic community of freshwater shallow lakes in the northern Victoria Land, East Antarctica

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