Morphology and Morphometry of Seminiferous Tubules in Crioulo Horses

Figueiró, Giuliano Moraes; Rubin, M. I. B.; Faria, F. J. C.; Santos, Marcelo Diniz dos; Fernandes, C. A. C.; Furtado, Fernando H. G.

doi:10.1016/j.jevs.2016.11.003

Cited by 2 publications

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References 14 publications

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“…Using the tubular morphology method, eight stages of seminiferous epithelium have been reported in ruminants and other mammals, such as red deer (Cervus elaphus) [ 7 ], goat (Capra hircus) [ 8 ], donkey (Equus asinus) and mule (Equus mulus mulus) [ 9 ], landrace boar [ 10 ], roe deer (Capreolus capreolus) [ 11 ], wild boar [ 12 ], and ‘crioulo' horse [ 13 ]. In addition to the tubular morphology method, characterization of the stages of the seminiferous epithelium cycle can be performed by an acrosomic system using the periodic acid Schiff (PAS) staining.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Seminiferous Epithelium Stages in the Wild Javan Muntjac (Muntiacus muntjak muntjak) Using the Tubular Morphology Method

Wahyuni

Gholib

Adnyane

et al. 2018

Veterinary Medicine International

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Stages of the seminiferous epithelium of the testis of the wild Javan muntjac (Muntiacus muntjak muntjak) in hard antler period were characterized based on the tubular morphology method. The number and the relative frequencies of seminiferous epithelium stages and the morphometry of germinal cell nuclei were identified microscopically. We identified eight stages of seminiferous epithelium in testicular tissue of the Javan muntjac and found that the relative frequencies of stages I to VIII were 14.87, 15.12, 17.75, 6.87, 7.37, 12.37, 13, and 12.62%, respectively. The diameter of the nuclei of germinal cells varied in each stage of seminiferous epithelium. Diplotene-stage primary spermatocytes had prominent and large nuclei ~8.97 ± 1.0 μm in stages III and IV. Pachytene primary spermatocytes appeared in most stages, except stage IV, whereas leptotene- and diplotene-stage primary spermatocytes were found in stages I and II, and III and IV, respectively. Round spermatids were observed in stages IV to VIII and in stage I but were absent in stages II and III, while elongated spermatids were observed in all stages except stage I. Our findings show that the stages of seminiferous epithelium in the Javan muntjac are similar to those found in neotropical cervids, small ruminants, and other domestic animals.

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Section: Introductionmentioning

confidence: 99%

Characterization of Seminiferous Epithelium Stages in the Wild Javan Muntjac (Muntiacus muntjak muntjak) Using the Tubular Morphology Method

Wahyuni

Gholib

Adnyane

et al. 2018

Veterinary Medicine International

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“…The tubular morphology method has been applied to many species, and eight stages of seminiferous tubules have been identified in the studies carried out in testis of goats (Capra hircus) [ 24 , 25 ], donkeys (Equus asinus) and mules (Equus mulus) [ 26 ], landrace boars (Sus scrofa domesticus) [ 27 ], crioulo horses (Equus ferus caballus) [ 28 ], dogs ( Canis familiaris) [ 29 ], and Javan muntjacs [ 18 ], whereas the second method has been applied to the testicular tissue of gerbils (Meriones unguiculatus) [ 30 ], long-tailed macaques [ 19 ], indigenous bull (Bos indicus) [ 31 ], mice (Mus musculus) [ 32 ], micromini pigs (Sus scrofa domesticus) [ 33 ], and rams [ 20 ]. However, the data regarding the stages of the seminiferous epithelium and spermatid development during spermatogenesis in the testis of Aceh bull have not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Identification and Determination of the Seminiferous Epithelium Stages and Spermatid Development in the Testis of Aceh Bull (Bos indicus)

Wahyuni,

Siregar,

Gholib

et al. 2023

Veterinary Medicine International

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This study was conducted to describe the stages of seminiferous epithelium (SE), determine the relative frequency of the stages, and identify the steps of spermatid development during spermatogenesis in the testicular tissue of Aceh bull. Seven pairs of the testicular organs of Aceh bull (Bos indicus) were used and then processed in a histological manner for staining using haematoxylin and eosin (H&E) and periodic acid-Schiff-haematoxylin (PAS-H). The stages of seminiferous tubules were examined using a tubular morphology method while spermatid development was observed based on the acrosome formation during spermatid development. Eight stages (stages I to VIII) of SE were found in the testicular seminiferous tubules of Aceh bull. Furthermore, the percentage of the relative frequency of each stage was 25.48, 15.38, 12.92, 4.74, 14.97, 10.69, 10.74, and 5.08%, respectively, with the relative frequency of premeiotic, meiotic, and postmeiotic phases being 53.78, 4.74, and 41.48%, respectively. Spermatid development from round to elongated spermatids occurred in 14 steps. Steps 1 to 7 were observed in stage I, steps 8 and 9 in stage II, steps 10 and 11 in stage III, step 12 in stage IV, step 13 in stages V and VI, and step 14 in stages VII and VIII. These findings can be used as a basis for further studies, particularly in evaluating the abnormality of the cellular composition of the seminiferous tubule in each stage of spermatogenesis and also in determining daily sperm production in Aceh bull.

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Morphology and Morphometry of Seminiferous Tubules in Crioulo Horses

Cited by 2 publications

References 14 publications

Characterization of Seminiferous Epithelium Stages in the Wild Javan Muntjac (Muntiacus muntjak muntjak) Using the Tubular Morphology Method

Characterization of Seminiferous Epithelium Stages in the Wild Javan Muntjac (Muntiacus muntjak muntjak) Using the Tubular Morphology Method

Identification and Determination of the Seminiferous Epithelium Stages and Spermatid Development in the Testis of Aceh Bull (Bos indicus)

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