Morphology and spatial distribution of bee antennal lobe interneurones responsive to odours

Fonta, Caroline; Sun, Xinde; Masson, C.

doi:10.1093/chemse/18.2.101

Cited by 126 publications

(128 citation statements)

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“…The staining within the antennal lobes presented here is very similar to previous stainings observed with rhodamine-dextran into the mushroom body (Schröter and Malun, 2000). The morphology of the projection neurons within the honeybee brain has been described in detail elsewhere (Homberg, 1984;Gascuel and Masson, 1991a;Fonta et al, 1993;Schröter and Malun, 2000;Abel et al, 2001). The staining procedure that we used labelled other neurons as well as antennal lobe projection neurons and Kenyon cells, including several known mushroom body extrinsic neurons (Mobbs, 1982(Mobbs, , 1985Rybak and Menzel, 1993), mushroom body feedback neurons (Bicker et al, 1985;Grünewald, 1999a) and, occasionally, a few somata within the suboesophageal ganglia.…”

Section: Identification and Culturing Of Projection Neuronssupporting

confidence: 82%

“…The axons of the olfactory receptor neurons terminate within the primary olfactory neuropils of the honeybee brain, the antennal lobes. Olfactory information is processed here in a complex spatio-temporal fashion (Joerges et al, 1997;Stopfer et al, 1997;Sachse et al, 1999) and projection neurons transmit olfactory information from the antennal lobes to the lateral protocerebral lobes and the mushroom bodies within the protocerebrum (Homberg, 1984;Fonta et al, 1993;Abel et al, 2001). In the mushroom body calyces the projection neurons synapse onto mushroom body-intrinsic Kenyon cells, named after their discoverer (Kenyon, 1896).…”

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confidence: 99%

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Differential expression of voltage-sensitive K+ and Ca2+ currents in neurons of the honeybee olfactory pathway

Grünewald

2003

Journal of Experimental Biology

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The formation of olfactory memory is a multistage process that involves different areas of the insect brain. Olfactory conditioning of the proboscis extension reflex of the honeybee has proved to be a very powerful system for studying learningrelated neural mechanisms, enabling major neural elements of the olfactory and reward pathways in the honeybee brain to be identified (Erber et al., 1980;Hammer, 1993;Mauelshagen, 1993;Grünewald, 1999b;Hammer and Menzel, 1998) (for reviews, see Hammer, 1997;Menzel, 1999Menzel, , 2001) and several molecular pathways involved in this behaviour to be elucidated (e.g. Hildebrandt and Müller, 1995;Müller, 1996Müller, , 2000Grünbaum and Müller, 1998;Fiala et al., 1999) (for a review, see Menzel and Müller, 1996). Odors are perceived by sensillae on the honeybee antennae. The axons of the olfactory receptor neurons terminate within the primary olfactory neuropils of the honeybee brain, the antennal lobes. Olfactory information is processed here in a complex spatio-temporal fashion (Joerges et al., 1997;Stopfer et al., 1997;Sachse et al., 1999) and projection neurons transmit olfactory information from the antennal lobes to the lateral protocerebral lobes and the mushroom bodies within the protocerebrum (Homberg, 1984;Fonta et al., 1993;Abel et al., 2001). In the mushroom body calyces the projection neurons synapse onto mushroom body-intrinsic Kenyon cells, named after their discoverer (Kenyon, 1896). Olfactory information converges here with information from other sensory modalities, such as visual input in the mushroom body, and with reward-processing modulatory neurons from the suboesophageal ganglion (Erber et al., 1987;Hammer and Menzel, 1995). Intracellular recordings showed that odor applications induce complex spiking patterns in projection neurons, thus encoding olfactory and gustatory sensory information (Stopfer et al., 1997;Abel et al., 2001;Müller et al., 2002). The underlying ion channels of projection neurons have not yet been analysed, however, because the neurons could not be identified and distinguished from local interneurons, as is possible in other insects (Hayashi and Hildebrand, 1990;Oland and Hayashi, 1993

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Section: Identification and Culturing Of Projection Neuronssupporting

confidence: 82%

mentioning

confidence: 99%

Differential expression of voltage-sensitive K+ and Ca2+ currents in neurons of the honeybee olfactory pathway

Grünewald

2003

Journal of Experimental Biology

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“…In contrast to honeybees (Fonta et al, 1993), most local neurons in Manduca show a typical broad "symmetrical" arborization pattern innervating the majority of AL glomeruli (Matsumoto and Hildebrand, 1981;Kuebler et al, 2011b;Reisenman et al, 2011). Consequently, an inhibitory global network incorporating a population of neurons with glomerulus-specific connectivity for general odor mixtures seems unlikely in Manduca (but compare the Manduca MGC: Christensen and Hildebrand, 1997;Lei et al, 2002;Heinbockel et al, 2004).…”

Section: Input/output Comparison Reveals Spatiotemporal Modulationmentioning

confidence: 98%

Antennal Lobe Processing Correlates to Moth Olfactory Behavior

Kuebler

Schubert²,

Kárpáti

et al. 2012

J. Neurosci.

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Animals typically perceive their olfactory environment as a complex blend of natural odor cues. In insects, the initial processing of odors occurs in the antennal lobe (AL). Afferent peripheral input from olfactory sensory neurons (OSNs) is modified via mostly inhibitory local interneurons (LNs) and transferred by projection neurons (PNs) to higher brain centers. Here we performed optophysiological studies in the AL of the moth, Manduca sexta, and recorded odor-evoked calcium changes in response to antennal stimulation with five monomolecular host volatiles and their artificial mixture. In a double staining approach, we simultaneously measured OSN network input in concert with PN output across the glomerular array. By comparing odor-evoked activity patterns and response intensities between the two processing levels, we show that host mixtures could generally be predicted from the linear summation of their components at the input of the AL, but output neurons established a unique, nonlinear spatial pattern separate from individual component identities. We then assessed whether particularly high levels of signal modulation correspond to behavioral relevance. One of our mixture components, phenyl acetaldehyde, evoked significant levels of nonlinear input-output modulation in observed spatiotemporal activation patterns that were unique from the other individual odorants tested. This compound also accelerated behavioral activity in subsequent wind tunnel tests, whereas another compound that did not exhibit high levels of modulation also did not affect behavior. These results suggest that the high degree of input-output modulation exhibited by the AL for specific odors can correlate to behavioral output.

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“…At the central level, sexual dimorphism is present at the first olfactory centre, the antennal lobe (AL). In worker bees, the AL consists of ~160 identified functional units, the glomeruli, which are interconnected by approximately 4000 local, inhibitory interneurons (Fonta et al, 1993). OSNs project onto such glomeruli via the antennal nerve and processed olfactory information leaves the AL by approximately 800 projection neurons, toward higher-order brain centres, such as the mushroom bodies or the lateral protocerebrum (Abel et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Odour-evoked responses to queen pheromone components and to plant odours using optical imaging in the antennal lobe of the honey bee droneApis melliferaL.

Sandoz

2006

Journal of Experimental Biology

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The primordial functional role of honey bee males (drones) is to mate with virgin queens, a behaviour relying heavily on the olfactory detection of queen pheromone. In the present work I studied olfactory processing in the drone antennal lobe (AL), the primary olfactory centre of the insect brain. In drones, the AL consists of about 103 ordinary glomeruli and four enlarged glomeruli, the macroglomeruli (MG). Two macroglomeruli (MG1 and MG2) and approximately 20 ordinary glomeruli occupy the anterior surface of the antennal lobe and are thus accessible to optical recordings. Calcium imaging was used to measure odour-evoked responses to queen pheromonal components and plant odours. MG2 responded specifically to the main component of the queen mandibular pheromone, 9-ODA. The secondary components HOB and HVA each triggered activity in one, but not the same, ordinary glomerulus. MG1 did not respond to any of the tested stimuli. Plant odours induced signals only in ordinary glomeruli in a combinatorial manner, as in workers. This study thus shows that the major queen pheromonal component is processed in the most voluminous macroglomerulus of the drone antennal lobe, and that plant odours, as well as some queen pheromonal components, are processed in ordinary glomeruli.

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Morphology and spatial distribution of bee antennal lobe interneurones responsive to odours

Cited by 126 publications

References 31 publications

Differential expression of voltage-sensitive K+ and Ca2+ currents in neurons of the honeybee olfactory pathway

Differential expression of voltage-sensitive K+ and Ca2+ currents in neurons of the honeybee olfactory pathway

Antennal Lobe Processing Correlates to Moth Olfactory Behavior

Odour-evoked responses to queen pheromone components and to plant odours using optical imaging in the antennal lobe of the honey bee droneApis melliferaL.

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