Morphology assessment and fluorescence in situ hybridization of the same spermatozoon using a computerized cell-scanning system

Strassburger, D.; Reichart, M.; Kaufman, Suzana; Kasterstein, E.; Komarovsky, D.; Bern, O.; Friedler, S.; Schächter, M; Ron-El, Raphaël; Raziel, Arieh

doi:10.1093/humrep/del357

Cited by 16 publications

(17 citation statements)

References 44 publications

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“…Similar results were observed in the study of Rives et al [20]. This is, however, in contrast to others studies [4,16,21], in which XX disomy was increased as opposed to YY disomy. Moreover, for these patients with severely teratozoospermia a significant correlation between aneuploidy rates and sperm morphology was found.…”

Section: Discussionsupporting

confidence: 89%

Detection of DNA fragmentation and meiotic segregation in human with isolated teratozoospermia

Brahem

Mogharrabi

Elghezal

et al. 2010

J Assist Reprod Genet

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Section: Discussionsupporting

confidence: 89%

Detection of DNA fragmentation and meiotic segregation in human with isolated teratozoospermia

Brahem

Mogharrabi

Elghezal

et al. 2010

J Assist Reprod Genet

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“…CCSS enables a maximal sampling of the whole extracted tissue while screening dozens of testicular cell, including post-meiotic cells, with a high level of confidence [25,26] and a screening of more than 1,000 spermatogonia and 900 primary spermatocytes for each of the 11 biopsy samples. As expected, based on lymphocyte karyotypes in non-mosaic KS men, and similar to Rives et al [11] and Yamamoto et al [23], who identified between 88.5 and 100 % 47,XXY spermatogonia in testicular biopsies, we found around 90 % spermatogonia with 47,XXY and 10 % with 46,XY, independently of whether or not patients produced testicular sperm.…”

Section: Discussionmentioning

confidence: 99%

“…It enables multisequential staining of cell preparations (Duet™, BioView Ltd, Nes-Ziona, Israel). This method provides large-scale automated bright field and fluorescence scanning for simultaneous classification of morphology and FISH in the same cell and for many cells [25,26].…”

Section: Assay Characteristicsmentioning

confidence: 99%

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Ploidy of spermatogenic cells of men with non-mosaic Klinefelter’s syndrome as measured by a computerized cell scanning system

Komsky-Elbaz

Raziel

Ben‐Ami

et al. 2015

J Assist Reprod Genet

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Purpose This study aims to characterize the origin of testicular post-meiotic cells in non-mosaic Klinefelter's syndrome (KS). Methods The study included testicular tissue specimens from 11 non-mosaic KS patients, with (6 positive) and without (5 negative) spermatozoa presence. The obtained testicular cells were affixed and stained for morphology followed by fluorescence in situ hybridization (FISH) for centromeric probes X, Y, and 18. We used a computerized automated cell scanning system that enables simultaneous viewing of morphology and FISH in the same cell. Results A total of 12,387 cells from the positive cases, 11,991 cells from the negative cases, and 1,711 cells from the controls were analyzed. The majority of spermatogonia were 47, XXY in both the positive and negative KS cases (88.9±4.76 % and 90.6±4.58 %) as were primary spermatocytes (76.8±8.14 % and 79.6±7.30 %). The respective rates of secondary spermatocytes and post-meiotic cells (round, elongating spermatids and sperm cells) were 1.1±1.39 % in the positive cases, 2.9±3.33 % in the negative cases, compared to 67.6±6.22 % in the controls (P<0.02). Pairing of both 18 and XY homologous chromosomes in 46,XY primary spermatocytes was 2.5 ±2.31 % and 3.4±2.39 %, respectively, compared to 19.8± 8.95 % in the control group (P<0.02) and in 47,XXY primary spermatocytes in 2.4±3.8 % in the positive group and 3.2± 2.26 % in the negative group. Conclusions This study presents data to indicate that the majority of primary spermatocytes in the testes of non-mosaic KS patients are 47,XXY and could possibly develop into postmeiotic cells.

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“…12,22 However, the computerized cell scanning system has demonstrated the relationship between chromosomal aberrations and sperm morphology in the same spermatozoon. 23 Furthermore, the studies supporting a relationship between sperm aneuploidy levels and sperm motility or sperm concentration contain methodological biases; namely, only a small number of patients were tested and/or few (one to three) autosomes1XY chromosomes were assessed and/or the statistical analyses used were based on thresholds, whereas sperm concentration, motility and morphology, and the percentage of aneuploid spermatozoa are continuous variables. 12,22 The absence of a placebo-treated group may be a potential source of bias in this study; therefore, we should consider that the treatment results attained may also be due to the vehicles used in the drug production.…”

Section: Discussionmentioning

confidence: 99%

Reduction in sperm aneuploidy levels in severe oligoasthenoteratospermic patients after medical therapy: a preliminary report

Cavallini¹,

Magli²,

Crippa³

et al. 2012

Asian J Androl

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The objective of this study was to investigate whether medical therapy can reduce sperm aneuploidy levels and improve the results of intracytoplasmic sperm injection (ICSI) in patients with severe idiopathic oligoasthenoteratospermia (OAT). Thirty-three infertile couples requiring ICSI because of severe idiopathic OAT after at least one unsuccessful ICSI cycle were considered. Semen parameters (concentration, motility and morphology), the percentage of aneuploid sperm and the results of ICSI (the number of oocytes fertilized, embryos transferred, biochemical pregnancies, clinical pregnancies and live births) were compared before and after a 3-month course of treatment with L-carnitine 1 g given twice per day1acetyl-L-carnitine 500 mg given twice per day1one 30-mg cinnoxicam tablet every 4 days. Aneuploidy was assessed using fluorescent in situ hybridisation (FISH) performed on chromosomes X, Y, 13, 15, 16, 17, 18, 21 and 22. The results showed that 22 of the 33 patients had a reduced frequency of aneuploid sperm and improved sperm morphology after treatment (group 1), and 11 showed no change (group 2). The numbers of biochemical pregnancies, clinical pregnancies and live births were significantly higher in group 1 than in group 2. No significant difference was found between the groups regarding the numbers of oocytes fertilized and embryos transferred. The side effects were negligible. The numbers of ICSI pregnancies and live births in severe idiopathic OAT patients improved with a course of L-carnitine, acetyl-L-carnitine and cinnoxicam.

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Morphology assessment and fluorescence in situ hybridization of the same spermatozoon using a computerized cell-scanning system

Cited by 16 publications

References 44 publications

Detection of DNA fragmentation and meiotic segregation in human with isolated teratozoospermia

Detection of DNA fragmentation and meiotic segregation in human with isolated teratozoospermia

Ploidy of spermatogenic cells of men with non-mosaic Klinefelter’s syndrome as measured by a computerized cell scanning system

Reduction in sperm aneuploidy levels in severe oligoasthenoteratospermic patients after medical therapy: a preliminary report

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