Morphology of cochlear root neurons in the rat

Merchán, Miguel Á.; Collía, Francisco; López, Dolores E.; Saldaña, Enrique

doi:10.1007/bf01260998

Cited by 66 publications

(58 citation statements)

References 26 publications

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“…Many fibers in the auditory nerve root were strongly immunopositive, as were large cells with oval somata and an eccentric nucleus that were likely to be root neurons ( Fig. 1F; Merchan et al, 1988). Thus, both primary afferent fibers and many first-order relay neurons were immunopositive for NOS-I.…”

Section: Nos-i Distributionmentioning

confidence: 90%

Immunohistochemical localization of nitric oxide synthase and soluble guanylyl cyclase in the ventral cochlear nucleus of the rat

Burette

Petrusz

Schmidt³

et al. 2001

J. Comp. Neurol.

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The diffusible messenger nitric oxide (NO) is implicated in auditory processing. It acts in the brain largely through activation of soluble guanylyl cyclase (sGC), a heterodimer comprised of alpha and beta subunits. The authors used immunohistochemistry to study the NO/guanosine 3',5'-cyclic monophosphate (cGMP) pathway in the cochlear nucleus of Sprague-Dawley rats. Central fibers of the cochlear nerve were stained for neuronal nitric oxide synthase (NOS-I) but not for sGCbeta. Within the ventral cochlear nucleus, a large fraction of principal cells were immunopositive for both NOS-I and sGCbeta; these cells could be seen at times receiving contacts from NOS-I-positive fibers. sGC staining of somatic cytoplasm extended into the distal dendritic tree. At variance with this pattern, NOS-I was concentrated mainly in somata. Double-labeling experiments showed that most of the principal neurons expressed both antigens. By contrast, in the granule cell domain, small cells that were immunopositive for NOS-I rarely corresponded to those that were immunopositive for sGC. To assess whether NOS-I and sGC immunoreactivities colocalize with their respective catalytic activities, the authors performed multiple labeling with L-citrulline (a by-product of the formation of NO from L-arginine) and cGMP, respectively. L-citrulline was restricted to NOS-I-positive elements, and the large majority of NOS-expressing neurons were positive for citrulline. Multiple labeling revealed that almost all sGC-positive neurons also accumulated cGMP both in the ventral cochlear nucleus and in the granule cell domain. These data suggest that NO is a signaling molecule in the cochlear nucleus, perhaps functioning in both a paracrine manner and an autocrine manner.

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Section: Nos-i Distributionmentioning

confidence: 90%

Immunohistochemical localization of nitric oxide synthase and soluble guanylyl cyclase in the ventral cochlear nucleus of the rat

Burette

Petrusz

Schmidt³

et al. 2001

J. Comp. Neurol.

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“…Corresponding cell types were later defined in rats and in cats (rat: Saldaña et al, 1987;Merchán et al, 1988;cat: Osen, 1969;Brawer et al, 1974). Five main types are generally recognized (Figs.…”

Section: Ventral Cochlear Nucleusmentioning

confidence: 96%

Auditory System

Malmierca

2015

The Rat Nervous System

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“…When the focus level is changed, stained fibers are seen approaching the soma. The sources of input to these neurons include primary afferents as well as afferents from noncochlear sources (Harrison and Warr, 1962;Merchá n et al, 1988;Osen et al, 1991). Which of these inputs are stained by Kv3.1 antibodies remains to be determined.…”

Section: Localization Of Kv31 Immunoreactivity In the Cnmentioning

confidence: 98%

Localization of a high threshold potassium channel in the rat cochlear nucleus

1997

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Potassium channels play a major role in determining the pattern and frequency of neuronal firing. In the cochlear nucleus (CN), various morphologically defined types of neurons have different responses to a sound. We have previously identified one type of cloned K+ channel, termed Kv3.1, which is highly expressed in many auditory neurons. Expression studies indicate that Kv3.1 channels have an unusually high threshold for activation. In this study, we used both in situ hybridization and immunohistochemistry to examine the expression patterns of the Kv3.1 channel in the CN. In the ventral CN, bushy cells hybridized strongly with Kv3.1 specific probes and a subpopulation of stellate/multipolar cells hybridized with Kv3.1 probes. In the dorsal CN, pyramidal and large multipolar/giant cells expressed Kv3.1 mRNA. Abundant Kv3.1 immunolabeling was also observed in the CN. The pattern of immunolabeling revealed that the Kv3.1 protein is distributed along the soma, proximal dendrites, unmyelinated axons, and axon terminals of stained neurons. In the case of pyramidal and octopus cells, no immunolabeling was detected at the somata, even though these cells expressed Kv3.1 mRNA. Computer simulations were used to explore the functional role of the Kv3.1 channel. The simulations indicate that Kv3.1 conductances may contribute to repolarization of large synaptic potentials. When stimulated at high frequencies, the presence of Kv3.1 enhances the ability of a model cell with some of the features of bushy cells to follow high frequency input with temporal precision.

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Morphology of cochlear root neurons in the rat

Cited by 66 publications

References 26 publications

Immunohistochemical localization of nitric oxide synthase and soluble guanylyl cyclase in the ventral cochlear nucleus of the rat

Immunohistochemical localization of nitric oxide synthase and soluble guanylyl cyclase in the ventral cochlear nucleus of the rat

Auditory System

Localization of a high threshold potassium channel in the rat cochlear nucleus

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