Morphology of epithelial cells lining the digestive tract of the giant keyhole limpet, <i>Megathura crenulata</i> (Mollusca; Vetigastropoda)

Martin, Gary G.; Bessette, Tracy; Martin, Alanna; Cotero, Renae; Vumbaco, Kathryn; Oakes, Christopher T.

doi:10.1002/jmor.10862

Cited by 13 publications

(10 citation statements)

References 32 publications

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“…3 and 4). We suggest that these cells may be ciliated cells, which are widely distributed in digestion system of mollusks (Kakoi et al, 2008;Martin et al, 2010). Immunofluorescence experiments further confirmed that the Cgi-Calaxin protein is distributed in the cilia of Dveligers.…”

Section: Discussionsupporting

confidence: 54%

A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia

Wang

Liu

et al. 2015

Zoological Science

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calaxin is a newly identified calcium sensor gene that modulates the movement of flagella (and possibly cilia). It was first identified from the ascidian Ciona intestinalis, and its orthologs have been observed in a wide range of animals and choanoflagellates. However, no calaxin-ortholog in a Lophotrochozoa species has been reported so far. This leaves open the question of whether the modulation of ciliary motility by calaxin is conserved among animals. We report a calaxin gene from the Pacific oyster Crassostrea gigas. This gene, termed cgi-calaxin, possesses three conserved EF-hand motifs as its orthologs from chordates. A phylogenetic analysis confirmed its orthology. Expression analysis revealed high expression in typical ciliated tissues such as gill and hepatopancreas. The spatiotemporal expression of the gene during early development was investigated using whole mount in situ hybridization. The results revealed that cgi-calaxin mRNA was aggregated in ciliated tissues of early larvae such as prototroch and velum. Immunofluorescence experiment further certified the ciliary localization of Cgi-Calaxin protein in D-veligers. We prepared the recombinant protein of cgi-calaxin and proved it had the capacity to bind to calcium. These results support the conserved roles of cgi-calaxin as a calcium sensor in ciliated cells and enrich the current knowledge on regulatory mechanisms of molluscan cilia. Moreover, we found the gene to be expressed in poorly ciliated tissues, such as adductor muscle, indicating possible roles in non-ciliated cells, which merits further investigation.

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Section: Discussionsupporting

confidence: 54%

A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia

Wang

Liu

et al. 2015

Zoological Science

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“…Results from the present study are consistent with earlier findings, which demonstrated that cells in the esophagus and the intestine are morphologically similar (Martin et al. ) and even secrete many of the same digestive enzymes (Martin et al. ).…”

Section: Discussionsupporting

confidence: 93%

“…Additional identification of the site of PM formation was based on histological observation of an acellular layer lying along the gut epithelium; this layer stained with PAS and WGA-FITC. Examination by SEM of gut regions revealed three basic morphologies, two as described previously (Martin et al 2010). In some regions, the surface of the epithelial cells was covered by cilia with interspersed spheres of apocrine secretion ( Fig.…”

Section: Secretion Of the Peritrophic Membranesupporting

confidence: 60%

“…Examination by SEM of gut regions revealed three basic morphologies, two as described previously (Martin et al. ). In some regions, the surface of the epithelial cells was covered by cilia with interspersed spheres of apocrine secretion (Fig.…”

Section: Resultsmentioning

confidence: 57%

“…These secretions were considered mucus granules in a previous study (Martin et al. ). Results from the present study are consistent with earlier findings, which demonstrated that cells in the esophagus and the intestine are morphologically similar (Martin et al.…”

Section: Discussionmentioning

confidence: 99%

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The peritrophic membrane of the gastropod Megathura crenulata: structure, composition, and site of formation

Valk

Schindelman

Martin

2014

Invertebrate Biology

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Peritrophic membranes (PMs) are acellular layered structures secreted around ingested materials by the gut epithelium. Most studies on PMs have focused on those of insects and crustaceans due to their potential ability to block the movement of pathogens from ingested materials into the body, and their possible use as unique targets relevant to pest management. While PMs are known to occur in other taxa, their distribution is spotty and little is known about their role in these other species. The gastropod Megathura crenulata produces a true PM, which has a chitinous matrix that makes up nearly half its wet weight. Unlike arthropod PMs, which are released by delamination from the microvilli of their gut cells, the chitinous matrix of the M. crenulata PM is secreted from epithelial cells lining most regions of its gut. Although its mode of synthesis is unique, it may serve the same functions as proposed for other PMs, including regulating diffusion, binding metabolites, restricting protease activity, blocking pathogens, and providing lubrication. In arthropods, numerous proteins with chitin‐binding specificities have been identified, consistent with the proposed functions. Analysis of PMs in M. crenulata showed several integral proteins associated with the membrane, suggesting that the PM in this mollusc may be involved in complex functions like those seen in the arthropods.

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Morphology of the digestive gland of the marine panpulmonate limpetSiphonaria lessonii: A cytological, histochemical, and ultrastructural description

Landro

Teso

Arrighetti

2019

Journal of Morphology

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The molluskan digestive gland has been widely studied and its structural and ultrastructural descriptions have allowed the understanding of its several functions. Despite siphonarids are broadly distributed around the world, morphological studies on their digestive system are poorly represented. The panpulmonate limpet Siphonaria lessonii is the most abundant gastropod and the dominant herbivore in the rocky intertidal coast of Buenos Aires. The aim of this study was to describe the morphology, histology, ultrastructure, and histochemistry of the digestive gland of this gastropod as well as the cycle of activity of digestion. For that, different histochemical techniques along with light microscopy, transmission electron microscopy, and scanning electron microscopy were employed. This study revealed a complex epithelium, composed of a simple layer with five cell types. Digestive cells and vacuolated cells are responsible for intracellular digestion and energy accumulation; basophilic cells, secrete substances that would be involved in extracellular digestion; pigmented cells might have an excretory function and thin cells would correspond to undifferentiated cells. In addition, the tubules present a changing morphology according to the digestive activity that they undergo. As S. lessonii is a grazer that feeds continuously, the cycle of activity of the digestive gland seems to be daily.

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Morphology of epithelial cells lining the digestive tract of the giant keyhole limpet, Megathura crenulata (Mollusca; Vetigastropoda)

Cited by 13 publications

References 32 publications

A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia

A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia

The peritrophic membrane of the gastropod Megathura crenulata: structure, composition, and site of formation

Morphology of the digestive gland of the marine panpulmonate limpetSiphonaria lessonii: A cytological, histochemical, and ultrastructural description

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