Morphology of phages of a general Salmonella typing set

Ackermann, Hans-Wolfgang; Gershman, M.

doi:10.1016/s0923-2516(06)80118-4

Cited by 17 publications

(9 citation statements)

References 9 publications

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“…The N-terminal regions of the TSPs are highly conserved and may represent the domain for binding to the phage base-plate, reminiscent of those of podoviruses, although this has not yet been confirmed experimentally. TSP1 and TSP3 share a common N-terminal region that shares extensive homology with a tail spike from phage Det7 [27], a phage that most likely belongs to the genus “Viunalikevirus”. The order of TSP1 and TSP3 with respect to TSP2 is conserved within this variable region of the ViI-group; only TSP1 and TSP3 have homology to the one Det7 spike whose sequence has been published to date [27], while no TSP2 genes have shown any homologies to that spike.…”

Section: Tail Spikesmentioning

confidence: 99%

“…TSP1 and TSP3 share a common N-terminal region that shares extensive homology with a tail spike from phage Det7 [27], a phage that most likely belongs to the genus “Viunalikevirus”. The order of TSP1 and TSP3 with respect to TSP2 is conserved within this variable region of the ViI-group; only TSP1 and TSP3 have homology to the one Det7 spike whose sequence has been published to date [27], while no TSP2 genes have shown any homologies to that spike. The TSP2 genes of the ViI-like phages (when present) share a highly homologous N-terminal domain, which to date has been found exclusively in viunalikeviruses.…”

Section: Tail Spikesmentioning

confidence: 99%

“…The second, C-terminal region can again be divided into two domains in at least some of the spikes, as seen most clearly in the crystal structure determined for this portion of the published Det7 spike [27]. The proximal beta-helical region has an enzymatic function, exemplified in Det7 by an O-antigen binding and hydrolysis domain, in ViI by an acetyl-esterase, which catalyzes the acetyl-modification of the Vi capsule, and in CBA120 by a section related to the N-acetylneuraminidase domain in the spike of coliphage K1F.…”

Section: Tail Spikesmentioning

confidence: 99%

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A suggested new bacteriophage genus: “Viunalikevirus”

et al. 2012

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We suggest a bacteriophage genus, “Viunalikevirus”, as a new genus within the family Myoviridae . To date, this genus includes seven sequenced members: Salmonella phages ViI, SFP10 and ΦSH19; Escherichia phages CBA120 and PhaxI; Shigella phage phiSboM-AG3; and Dickeya phage LIMEstone1. Their shared myovirus morphology, with comparable head sizes and tail dimensions, and genome organization are considered distinguishing features. They appear to have conserved regulatory sequences, a horizontally acquired tRNA set and the probable substitution of an alternate base for thymine in the DNA. A close examination of the tail spike region in the DNA revealed four distinct tail spike proteins, an arrangement which might lead to the umbrella-like structures of the tails visible on electron micrographs. These properties set the suggested genus apart from the recently ratified subfamily Tevenvirinae , although a significant evolutionary relationship can be observed. Electronic supplementary material The online version of this article (doi:10.1007/s00705-012-1360-5) contains supplementary material, which is available to authorized users.

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Section: Tail Spikesmentioning

confidence: 99%

Section: Tail Spikesmentioning

confidence: 99%

Section: Tail Spikesmentioning

confidence: 99%

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A suggested new bacteriophage genus: “Viunalikevirus”

et al. 2012

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“…The Jersyvirine subfamily include a distinct morphotype, genomes of 40–44 kb (49.6-51.4 mol % G + C), a syntenic genome organization, high degree of nucleotide sequence identity, and strictly lytic cycle [30]. As mentioned previously, the Siphoviriade family presents considerable mosaicism [31, 32] and although we distinguished a possible new genus for the subfamily Jersyvirinae (Fig.…”

Section: Discussionmentioning

confidence: 84%

Complete genome sequence of the salmonella enterica serovar enteritidis bacteriophages fSE1C and fSE4C isolated from food matrices

Santander

Vásquez

Segovia

et al. 2017

Stand in Genomic Sci

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Salmonella enterica serovar Enteritidis is one of the most common causes of Salmonellosis worldwide. Utilization of bacteriophages as prophylactic agents is a practical solution to prevent Salmonellosis in ready-to-eat products. Shelf stability is one of the desirable properties for prophylactic bacteriophages. Here, we describe the phenotype, genome, and phylogeny of fSE1C and fSE4S Salmonella bacteriophages. fSE1C and fSE4S were previously isolated from pickle sauce and ground beef respectively and selected for their significant shelf stability. fSE1C and fSE4S showed a broad S. enterica serovar range, infecting several Salmonella serovars. The viral particles showed an icosahedral head structure and flexible tail, a typical morphology of the Siphoviridae family. fSE1C and fSE4C genomes consists of dsDNA of 41,720 bp and 41,768 bp with 49.73% and 49.78% G + C, respectively. Comparative genomic analysis reveals a mosaic relationship between S. enterica serovar Enteritidis phages isolated from Valparaiso, Chile.

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“…It is of characteristic mostly for long-tailed Enterococcus and Salmonella phages that have greatly elongated tails with their length being several times bigger than the diameter of the head [3,16]. The majority of Siphoviridae phages have rigid tails, e.g.…”

Section: Fig 9 the View Of Phage Key Discs On Partly Destroyed Tailmentioning

confidence: 99%

Virion Morphology and Structural Organization of Polyvalent Bacteriophages ТT10-27 and КEY

Faidiuk¹,

Boyko²,

Muchnyk³

et al. 2015

Mikrobiol. Z.

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Fine ultrastructure ofpolyvalent bacteriophages TT10-27 and KEY, isolated from affected with fire blight disease plant tissues, was studied using electron microscopy. Phages have isometric heads connected to short complex tail Key words: bacteriophages, Erwinia amylovora, morphology and structural organization o f virion.Morphological organization and ultrastructure of a phage particle is common criterion of bacteriophage classification and taxonomy [2]. According to Ackermann [2] phages of the order Caudovirales are subdivided into three families Myoviridae, Siphoviridae and Podoviridae due to their tail structure, and into subtypes according to head length. Tykhonenko [25] proposed phage classification based on the base plate shape or tail distal end if a base plate is absent. This approach allows the taxa of lower rank than family to be distinguished. Currently such classic criteria are not taken into consideration, however genomic and post-genomic studies are impossible without characterization of morphology of the object.Bacteriophages specific to Erwinia amylovora and other phytopathogenic and plantassociated bacteria were isolated from fire blight disease affected tissues of trees earlier [23]. Phages of two morphotypes: B1 (Siphoviridae) and C1 (Podoviridae) were observed among them [24]. Here we report results of electron microscopic studies of two representatives of the groups: phages TT10-27 and KEY Materials and methods.Bacteriophage TT10-27 was obtained when an isolate pMG (extracted from affected pear sample) was propagated on Erwinia "horticola" 60-1n and 4311 laboratory strains; phage KEY was obtained during reproduction of isolate pMA1 (extracted from affected quince) on E. amylovora K8(ATCC 29850) [23].Bacteriophages were obtained by fused lysis procedure [1] or by delayed lysis method de scribed in [24]. Phage concentration procedure included lysates clarification by centrifugation for 1h at 5000g, 10°C, with next phage particles sedimentation in rotor SW 28 Spinco L7-70 at 26000 rpm for 2 hours at 10°C. Obtained precipitate was re-suspended in STM buffer [18] and purified from cell debris and particles' conglomerates on microcentrifuge ELMI at 11000 rpm for 10 min.More thorough purification of virions was achieved using CsCl stepwise density-gradient centrifugation (1,42 g/cm3 i 1,60g/cm3). CsCl solution (5M) was prepared in buffer STM and in water. The latter contributed to the phage virions' destruction. Phage suspension of 0.5-1ml volume was applied on the gradient and centrifuged for 3 hours in SW 55 rotor, at 30 000 rpm, 10 °C.Dialysis of CsCl-purified virus was carried out against a 1000-fold excess of ST buffer [18] for 24 hours at 4 °C.

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Morphology of phages of a general Salmonella typing set

Cited by 17 publications

References 9 publications

A suggested new bacteriophage genus: “Viunalikevirus”

A suggested new bacteriophage genus: “Viunalikevirus”

Complete genome sequence of the salmonella enterica serovar enteritidis bacteriophages fSE1C and fSE4C isolated from food matrices

Virion Morphology and Structural Organization of Polyvalent Bacteriophages ТT10-27 and КEY

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