Morphology of rat brain neurons in subtotal ischaemia and introduction of L-NAME and omega-3 polyunsaturated fatty acids

Bon, Lizaveta I.; Максимович, Н. Е.; Зиматкин, С. М.

doi:10.20883/medical.e423

Cited by 6 publications

(7 citation statements)

References 11 publications

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“…Differences between groups were considered significant at p <0.05 (Kruskell-Wallis test with Bonferoni's correction) [19].…”

Section: Discussionmentioning

confidence: 99%

"Disorders of Energy Metabolism in Neurons of the Cerebral Cortex During Cerebral Ischemia"

Bon¹

2021

BJSTR

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Objective: Evaluation of changes in the content of ATP synthase in the parietal cortex and hippocampus of the brain of rats with ischemia of varying severity in a comparative aspect. Methods:The experiments were performed on 88 male outbred white rats weighing 260 ± 20 g. Brain ischemia was modeled under conditions of intravenous thiopental anesthesia (40-50 mg / kg). Total cerebral ischemia was modeled by decapitation of animals. The brain sampling was carried out 1 hour and 24 hours after decapitation -to study tissue respiration of mitochondria, as well as 1 hour later to determine the content of ATP synthase. Subtotal cerebral ischemia was modeled by simultaneous ligation of both common carotid arteries. The material was taken after 1 hour to determine the content of ATP synthase. Stepwise subtotal cerebral ischemia was performed by sequential ligation of both common carotid arteries with an interval of 7 days. The sampling was carried out 1 hour after ligation of the second common carotid artery in each of the subgroups. Partial cerebral ischemia was modeled by ligation of one common carotid artery on the right. The sampling was carried out 1 hour after the operation. Determination of the content of ATP synthase was carried out by immunohistochemical method using monoclonal antibodies. For this purpose, after decapitation, the brain was quickly removed from the rats, pieces of the cerebral cortex were fixed in zinc-ethanolformaldehyde at + 4 ° C (overnight), then embedded in paraffin. Results:In the group of stepwise subtotal cerebral ischemia, the smallest decrease in the content of ATP synthase was observed in the 1 st subgroup with an interval between dressings of 7 days, while the greatest decrease in the content of the enzyme was noted in the 3 rd subgroup with the minimum interval between the dressings of the common carotid artery (1 day). Modeling of more severe types of ischemic damage led to pronounced morphological changes in neurons in the parietal cortex and hippocampus of the rat brain -a decrease in their size, deformation of the perikarya, an increase in the degree of neuronal chromatophilia with their simultaneous wrinkling and subsequent death. These disorders were most pronounced in the 3 rd subgroup of stepwise subtotal

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“…Differences between groups were considered significant at p <0.05 (Kruskell-Wallis test with Bonferoni's correction) [19].…”

Section: Discussionmentioning

confidence: 99%

"Disorders of Energy Metabolism in Neurons of the Cerebral Cortex During Cerebral Ischemia"

Bon¹

2021

BJSTR

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“…Data are presented as Me (LQ; UQ), where Me is the median, LQ is the value of the lower quartile; UQ is the upper quartile value. Differences between groups were considered significant at p <0.05 (Kruskell-Wallis test with Bonferoni's correction) [19].…”

Section: Methodsmentioning

confidence: 99%

The content of ATP Synthase in Cerebral Ischemia of Varying Severity Comparative Analysis

I.¹,

N.E.,²,

A.³

et al. 2022

BTBP

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Objective. Evaluation of changes in the content of ATP synthase in the parietal cortex and hippocampus of the brain of rats with ischemia of varying severity in a comparative aspect. Methods. The experiments were performed on 88 male outbred white rats weighing 260 ± 20 g. Brain ischemia was modeled under conditions of intravenous thiopental anesthesia (40-50 mg / kg). Total cerebral ischemia was modeled by decapitation of animals. The brain sampling was carried out 1 hour and 24 hours after decapitation - to study tissue respiration of mitochondria, as well as 1 hour later to determine the content of ATP synthase. Subtotal cerebral ischemia was modeled by simultaneous ligation of both common carotid arteries. The material was taken after 1 hour to determine the content of ATP synthase. Stepwise subtotal cerebral ischemia was performed by sequential ligation of both common carotid arteries with an interval of 7 days. The sampling was carried out 1 hour after ligation of the second common carotid artery in each of the subgroups. Partial cerebral ischemia was modeled by ligation of one common carotid artery on the right. The sampling was carried out 1 hour after the operation. Determination of the content of ATP synthase was carried out by immunohistochemical method using monoclonal antibodies. For this purpose, after decapitation, the brain was quickly removed from the rats, pieces of the cerebral cortex were fixed in zinc-ethanol-formaldehyde at + 4 ° C (overnight), then embeddedвinвparaffin. Results. In the group of stepwise subtotal cerebral ischemia, the smallest decrease in the content of ATP synthase was observed in the 1st subgroup with an interval between dressings of 7 days, while the greatest decrease in the content of the enzyme was noted in the 3rd subgroup with the minimum interval between the dressings of the common carotid artery (1 day). Modeling of more severe types of ischemic damage led to pronounced morphological changes in neurons in the parietal cortex and hippocampus of the rat brain - a decrease in their size, deformation of the perikarya, an increase in the degree of neuronal chromatophilia with their simultaneous wrinkling and subsequent death. These disorders were most pronounced in the 3rd subgroup of stepwise subtotal cerebral ischemia with the shortest interval between dressings, which was 1 day, and in the group of total cerebral ischemia. Conclusion. Thus, the most pronounced decrease in the content of ATP synthase was observed in the groups of total cerebral ischemia, subtotal cerebral ischemia and in the 3rd subgroup of stepwise subtotal cerebral ischemia, with a minimal time interval between the ligation of the common carotid artery. In stepwise subtotal cerebral ischemia with an interval between ligation of the common carotid artery of 7 days, the suppression of the ATP synthase content was not so significant.

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“…Ngb is expressed mainly in the nervous system, retina and some endocrine structures [1,2,3]. The similarity of the Ngb structure with other globin proteins [4] suggests similar functions of providing oxygen homeostasis of cells [5]. But since the level of affinity of Ngb to oxygen is very high, and this is an obstacle to the release of oxygen, this metalloprotein is more often considered as an indicator of the oxygen level in mitochondria that oxidize organic substances and form ATP [6,38].…”

Section: Introductionmentioning

confidence: 99%

Effects of Neuroglobin in Cerebral Ischemia and Introduction of Omega-3 Polyunsaturated Fatty Acids

Bon¹

2023

PPD

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The aim of the work was to evaluate the content of neuroglobin in the brain of rats with cerebral ischemia and under the conditions of administration of omega-3 polyunsaturated fatty acids. The content of neuroglobin in the cytoplasm of neurons in the parietal cortex and hippocampus of rats under conditions of cerebral ischemia and administration of omega-3 polyunsaturated fatty acids was studied. Against the background of the introduction of the drug ω-3 PUFAs, there was an improvement in the indicators of antioxidant protection of brain neurons, which was manifested by an increase in the content of neuroglobin in hippocampal neurons.

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Morphology of rat brain neurons in subtotal ischaemia and introduction of L-NAME and omega-3 polyunsaturated fatty acids

Cited by 6 publications

References 11 publications

"Disorders of Energy Metabolism in Neurons of the Cerebral Cortex During Cerebral Ischemia"

"Disorders of Energy Metabolism in Neurons of the Cerebral Cortex During Cerebral Ischemia"

The content of ATP Synthase in Cerebral Ischemia of Varying Severity Comparative Analysis

Effects of Neuroglobin in Cerebral Ischemia and Introduction of Omega-3 Polyunsaturated Fatty Acids

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