Morphology of regressive changes in the kidney following experimental ischaemia

Abstract

Total or partial renal ischaemia was studied in rats and pigs in a series of experiments, after total devascularization (ligature of renal vessels and ureter); ligature of isolated renal artery; experimental embolization of renal artery by non-resorbable material; devascularization of a specific renal segment by deep, strangulating sutures. Morphological studies showed that the resulting necrotization is represented most frequently by coagulation necrosis, less frequently by colliquating necrosis. Occlusion of the renal artery by embolization or ligature leads only to partial necrotization or atrophy. Total devascularization of the renal hilus or total devascularization of a specific renal segment by strangulating sutures leads to total destruction of the devascularized (devitalized) tissues. Within 8-12 weeks, the whole organ changes into a small residue of connective tissue. The possibility of the use of the devascularization technique in the treatment of tumours is discussed.

Autologous tumor immunizing devascularization of an invasive colorectal cancer: A case report and literature review

“…On the macroscopic level, the devascularized tissue first increases in size; subsequently, shrinking occurs, and the devascularized tissue is eventually replaced by fibrous tissue (4). On the microscopic level, during the first 2 days the numbers of infiltrating mono- and polynuclear lymphocytes are observed to increase (3).…”

“…Furthermore, replacement with fibrous tissue has been observed. Examples taken from animal studies include transplantation of the hypophysis following total resection in the rectus muscle, bone marrow of the tibia and cortex cerebri in dogs (n=23), thereby prolonging life compared with resection alone (9), the ligation of intestines up to 180 cm in length in pigs (n=47) (3,10) without any side-effects, even following injection of pathogenic bacteria into the stomachs of pigs (n=23) (11), and the ligation of parathyroid glandules in dogs (n=14) (12), the spleen in rats (n=35) (13) and a guinea-pig (n=1) (14), kidneys in pigs (n=21) and rats (n=60) (4), testes in dogs (n=3) (15) and cocks (n=4) (16), and ovaries in dogs (n=6) (17). Following devascularization of the colon, no migration of bacteria through the ischemic wall was observed for up to 6 h (18).…”

“…Obduction revealed no metastases, and, in both cases, icterus (jaundice) was the cause of death. In a 65-year-old man who had a kidney adenocarcinoma with infiltration of the peritoneum, ATID was successfully performed, and the patient enjoyed 3 years of good health (4). He finally succumbed to a cerebral hemorrhage caused by high blood pressure.…”

Melanoma-Bearing Libechov Minipig (MeLiM): The Unique Swine Model of Hereditary Metastatic Melanoma

“…Recently, another case report of a patient with invasive metastatic colorectal carcinoma who survived more than 14 years after devitalization, with no sign of malignancy revealed on computed tomography (CT) scans at present, was published [253]. Using healthy minipigs in IAPG as an anatomical and physiological model similar to human, devitalization of the kidney [254,255], stomach [256], rectum, and sigmoideum [257] was also carried out to acquire practical skills and experimental knowledge as a prerequisite for possible clinical utilization. In all cases, the devitalized tissues were resorbed and no side effects were observed.…”