Morphology-related effects on gene expression and protein accumulation of the yeast Arxula adeninivorans LS3

Abstract

The dimorphism of the yeast Arxula adeninivorans LS3 is regulated by cultivation temperatures. Up to 42 degrees C the yeast grows as budding cells, which turn to mycelia at higher temperatures. To test whether the dimorphism is exclusively induced by high temperatures or also by other conditions, mutants were selected with an altered behaviour with respect to dimorphism. After mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, five of 25,000 colonies formed a very rough surface consisting of mycelia at 30 degrees C, in contrast to the wild-type. These mutants allow temperature-mediated and morphology-related effects on gene expression and protein accumulation to be distinguished. Budding cells and mycelia showed different expression of genes encoding secretory proteins at the same temperature. Mycelia secreted two-fold more protein than budding cells, including the enzymes glucoamylase and invertase. This indicated that morphology, rather than temperature, is the decisive factor in the analysed processes.

General factors important for the formation of structured biofilm-like yeast colonies

“…This data clearly shows that the polarity of budding and shape of S. cerevisiae cells are not essential prerequisites for the formation of distinct colony morphology. This finding is in direct contrast to previous studies of some nonconventional yeasts, where a clear link between colony and cell morphology was described (see also Section 1) (Radford et al, 1994;Wartmann et al, 2000).…”

Role of theAFRD1-encoded fumarate reductase in hypoxia and osmotolerance inArxula adeninivorans

“…In this study, the wild-type A. adeninivorans strain, LS3 [originally isolated from wood hydrolysates in Siberia and deposited as A. adeninivorans SBUG 724 into the strain collection of the Department of Biology of the University of Greifswald (SBUG)], was used together with the auxotrophic mutant A. adeninivorans G1211 (Kunze & Kunze, 1994;Samsonova et al, 1996;Wartmann et al, 2000;Steinborn et al, 2007). Both strains were grown up to 216 h at 30°C (Tanaka et al, 1967;Rose et al, 1990) under nonselective conditions in a complex medium (YEPD) or under selective conditions in a yeast minimal medium (YMM) supplemented with 2% glucose (YMM glucose).…”

Application of a wide-range yeast vector (CoMed™) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts

“…Plasmid DNA, restriction fragment isolation, labeling of fragments and Southern transfer were carried out as previously described by [32,65]. …”