Morphology, Solid Structure and Antioxidant Activity in Vitro of Angelica sinensis Polysaccharide‐Ce(IV)

Abstract

Angelica Sinensis polysaccharide cerium (ASP−Ce) was prepared by Angelica Sinensis polysaccharide (ASP) and cerium ammonium nitrate (NH4)2Ce(NO3)6. and its morphology, and solid structure was investigated. The antioxidant activity of the ASP−Ce complex in vitro was evaluated. The antioxidant activity of ASP−Ce complex in vitro was evaluated by the scavenging activity of 2,2‐diphenyl‐1‐picrylhyrazyl radical (DPPH), hydroxyl radical (⋅OH) and superoxide anion radical ( normalO2- ${{{\rm O}}_{2}^{-}}$ ⋅). The results showed that the ASP−Ce had the more ordered structure for inserting the Ce4+ into the polymer chain of ASP and there was little change in the conformation of the polysaccharide from Ce4+. Three free radical scavenging experiments proved that ASP−Ce had better antioxidant capacity than of ASP, especially on DPPH, and then on normalO2- ${{{\rm O}}_{2}^{-}}$ ⋅. The scavenging rate of ASP−Ce at 1.0 mg/mL on DPPH reached 71.6 %. Therefore, these results provide references for the further development and utilization of rare earth‐polysaccharide.

Effect of tremella polysaccharides on the quality of collagen jelly: insight into the improvement of the gel properties and the antioxidant activity of yak skin gelatin

“…The determination of the hydroxyl (OH) radical scavenging activity was obtained following the method of Li et al 33 . with minor modifications.…”

“…The determination of the hydroxyl (OH) radical scavenging activity was obtained following the method of Li et al 33 with minor modifications. Briefly, 0.4 mL of 9 mmol L −1 iron(II) sulfate (FeSO 4 ), 0.4 mL of 9 mmol L −1 salicylic acid-ethanol and 0.4 mL of 3% hydrogen peroxide (H 2 O 2 ) were added to 0.4 mL of 1 mg mL −1 sample solution in sequence.…”