Morphology, wood structure and cell wall composition of rolC transgenic and non-transformed aspen trees

Grünwald, C.; Ruel, K.; Joseleau, J.P.; Fladung, Matthias

doi:10.1007/s00468-001-0126-6

Cited by 17 publications

(13 citation statements)

References 42 publications

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“…Work along this line includes studies of the deposition of cell wall components during the differentiation of xylem cells in normal and 35S-rolC transgenic hybrid aspen Populus tremula 9 P. tremuloides, (Dünisch et al 2006;Grünwald et al 2001Grünwald et al , 2002a, which has a dwarfed phenotype. In the wild-type, polysaccharides were deposited before the lignin during cell wall formation, while these two events proceeded more in parallel in the transgenic plants.…”

Section: Uv-vis Spectra Of Lignin and Other Ring Structuresmentioning

confidence: 99%

Microspectroscopy as applied to the study of wood molecular structure

Fackler

Thygesen

2012

Wood Sci Technol

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Microspectroscopy gives access to spatially resolved information on the molecular structure and chemical composition of a material. For a highly heterogeneous and anisotropic material like wood, such information is essential when assessing structure/property relationships such as moisture-induced dimensional changes, decay resistance or mechanical properties. It is, however, important to choose the right technique for the purpose at hand and to apply it in a suitable way if any new insights are to be gained. This review presents and compares three different microspectroscopic techniques: infrared, Raman and ultraviolet. Issues such as sample preparation, spatial resolution, data acquisition and extraction of knowledge from the spectral data are discussed. Additionally, an overview of applications in wood science is given for each method. Lastly, current trends and challenges within microspectroscopy of wood are discussed.

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Section: Uv-vis Spectra Of Lignin and Other Ring Structuresmentioning

confidence: 99%

Microspectroscopy as applied to the study of wood molecular structure

Fackler

Thygesen

2012

Wood Sci Technol

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“…Differences in the composition of lignin in the various layers of the xylem cell wall have an effect on fiber properties and hence the quality of paper pulp. Variations in the topochemistry of condensed and non-condensed lignin substructures have already been revealed in hardwood lignin (Ruel et al 1999;Gru¨nwald et al 2001) and in Gramineae (Joseleau and Ruel 1997), by the use of different immunological probes raised against synthetic lignin polymers (Ruel et al 1994;Joseleau and Ruel 1997). In this study, a specific antibody against the dibenzodioxocin substructure was generated and used as a probe, allowing a detailed analysis of the lignin composition in softwood xylem cell walls.…”

Section: Introductionmentioning

confidence: 96%

Localization of dibenzodioxocin substructures in lignifying Norway spruce xylem by transmission electron microscopy–immunogold labeling

Kukkola

Koutaniemi

Gustafsson

et al. 2003

Planta

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“…Microscopic analyses did not reveal significant differences in the structure of xylem cell walls between 35S-rolC transgenic and wild type trees. Although histometric measurements have shown slightly reduced vessel lumen areas and fibre lengths as well as thinner fibre cell walls in the 35S-rolC transgenic than in the wild type trees, no evidence was found for an alteration of cell wall chemistry in the differentiated wood [15,16]. Therefore, it can be concluded that in the wild type trees lignification was not completed within 40 cell rows from the cambium, even in middle lamella regions, which are known to lignify more rapidly and intensely than the secondary walls [36].…”

Section: Discussionmentioning

confidence: 90%

“…In the 35S-rolC transgenic aspen trees used in the present study, physiological and morphological traits were distinctly modified [12,13]. In particular, in the xylem tissue no typical latewood was formed and an unusual high amount of gelatinous fibres was conspicuous; at the cellular level the general morphology of fibres, vessels and parenchyma cells appeared unchanged, although the formation and differentiation of these cells was delayed [15,16].…”

Section: Introductionmentioning

confidence: 95%

Differentiation of xylem cells in rolC transgenic aspen trees - a study of secondary cell wall development

Grünwald

Ruel

Schmitt³

2002

Ann. For. Sci.

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-Xylem cell differentiation of 35S-rolC transgenic aspen was analyzed and compared with wild type trees. The transgenic trees, mainly characterized by dwarfism and physiological alterations, did not show distinctive differences in the structure and chemical composition of xylem cell walls as compared with the wild type trees. However, there was a difference in the dynamics of differentiation. In the transgenics the formation of xylem cells was delayed and the differentiation zone reduced to a few rows. PATAg and KMnO 4 staining in electron microscopy as well as UV-microspectrophotometry revealed that in the transgenics during secondary wall formation polysaccharides and lignin were simultaneously deposited close to the cambium. Immunocytochemical techniques have visualized the first deposition of condensed lignin in cell corners and of non-condensed lignin in secondary walls near cell corners. Because of altered formation and differentiation of xylem cells, 35S-rolC transgenic aspen may be useful to study factors controlling the differentiation continuum.Populus / rolC / wood formation / lignification / electron microscopy / immunogoldlabelling / UV-microspectrophotometry Résumé -Différenciation des cellules du xylème chez le peuplier transgénique 35S-rolC -une étude de la formation des parois secondaires. La différenciation des cellules du xylème du peuplier transgénique 35S-rolC a été analysée et comparée à celle du peuplier non transformé. Les arbres transgéniques nains, qui se caractérisent par des altérations physiologiques et morphologiques ne présentaient pas de différences dans leur architecture ni dans la composition des parois des cellules du xylème par comparaison avec les plantes normales. En revanche, on a pu observer une différence dans la dynamique de la différenciation. Dans les arbres transgéniques, la formation des cellules du xylème est retardée et la zone de différenciation est réduite à quelques rangées de cellules. De ce fait, la formation et la lignification de la paroi secondaire ont pu être observées dans des cellules moins développées que dans le peuplier normal ainsi que cela a été mis en évidence par microspectrophotométrie-UV et par les contrastants PATAg et KMnO 4 en microscopie électronique à transmission. Des analyses immunocytochimiques ont révélé la toute première déposition de lignines condensées dans les jonctions intercellulaires et de lignines non condensées dans les parois secondaires adjacentes aux triangles de jonctions. Ces altérations dans la formation et la différenciation des cellules du xylème font du peuplier transgénique 35S-rolC un matériel utile pour l'étude des facteurs contrôlant le continuum de différenciation.Populus / rolC / formation des cellules du xylème / lignification / microscopie électronique à transmission / immunocytochimie / microspectrophotométrie-UV

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Morphology, wood structure and cell wall composition of rolC transgenic and non-transformed aspen trees

Cited by 17 publications

References 42 publications

Microspectroscopy as applied to the study of wood molecular structure

Microspectroscopy as applied to the study of wood molecular structure

Localization of dibenzodioxocin substructures in lignifying Norway spruce xylem by transmission electron microscopy–immunogold labeling

Differentiation of xylem cells in rolC transgenic aspen trees - a study of secondary cell wall development

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