Morphometric analysis of atypical glandular cells correctly classifies normal, reactive, and atypical cells in cervical smears

Vicari, Marco; Guidobaldi, Leo; Perrella, Eleonora; Bianchi, Antonella; Ferrari, Simona; Martucci, Mariarosaria; Petitti, Tommasangelo; Rabitti, Carla; Crescenzi, Anna

doi:10.1002/dc.24320

Cited by 1 publication

(1 citation statement)

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“…Examination and morphology of nucleus and cell boundaries, nucleus and cell area, width, length, nuclear‐to‐cytoplasm ratio, chromatin architecture, and staining density were examined in some studies 22‐26 . Many morphometric parameters helped classify cervical precancerous cells 40 . Few studies which have studied the association between MN scoring, cytomorphometry, and HPV in cervical non‐neoplastic, pre‐neoplastic, and neoplastic conditions are available in the literature.…”

Section: Discussionmentioning

confidence: 99%

Cytomorphometry and morphology analysis of human papillomavirus type 16 in liquid‐based cervical cytology samples

Öz

Kandemır

Köktürk

et al. 2021

Diagnostic Cytopathology

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BackgroundHuman papillomavirus (HPV) is the main causal factor of cervical carcinoma. HPV 16 is one of the most prominent oncogenic types. We aimed to evaluate the cytomorphometric and morphological alterations caused by HPV 16 in liquid‐based cytology (LBC).MethodsThe Cobas 4800 HPV system was used for the detecting and typing HPV DNA in cervical specimens. In this study, 30 HPV 16 positive and 30 HPV 16 negative cervical samples were evaluated for micronuclei (MN), nonclassical cytologic abnormalities, and the nuclear‐to‐cytoplasmic ratio. Nuclear and cellular areas were evaluated using image analysis software and the nuclear‐to‐cytoplasm ratio was calculated. All analyses were performed blinded to the patients' HPV status. Statistical evaluation was carried out using the χ2 and Fisher test; P‐values < .05 were considered significant.ResultsThe frequencies of micronucleated cells and koilocytes were higher in the HPV 16 infected group (P < .05). Cells with perinuclear halo in control group were higher than the HPV 16 infected group (P < .05). The mean nuclear‐to‐cytoplasm ratio in HPV 16 patients was higher than the control value, but the difference was not statistically significant.ConclusionLBC can be used to detect morphological and morphometric changes. HPV 16 induces the formation of MN and koilocytosis. The evaluation of MN could provide additional information in monitoring genomic instability and of koilocytes could provide information about damage to the cytoskeleton filaments in HPV infection. Further studies are needed to investigate the effects of HPV‐18 and other high‐risk HPV types on the cell size and nucleus‐to‐cytoplasm ratio.

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Section: Discussionmentioning

confidence: 99%