Morphometric analysis of gastric dysplasia

Jarvis, L. R.; Whitehead, R.

doi:10.1002/path.1711470209

Cited by 35 publications

(11 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The nuclear area is enlarged [63], cell cycle time is lengthened, and the cell life span is shortened [64]. Carcinoembryonic antigen and occasionally, p21ras may be present [65].…”

Section: Precancerous Lesions: Epithelial Dysplasiamentioning

confidence: 99%

Cellular and molecular pathology of gastric carcinoma and precursor lesions: A critical review

Ming

1998

Gastric Cancer

View full text Add to dashboard Cite

Abstract:The cellular and molecular pathology of gastric cancer and its precursors are reviewed and discussed. Gastric carcinogenesis is a multistep phenomenon, beginning with precancerous conditions. Among these, adenoma is a direct precursor, because of the dysplastic nature of its cells. However, gastric adenoma is relatively rare. Chronic atrophic gastritis (CAG) is the most common precancerous condition, in which intestinal metaplasia often occurs. Carcinoma develops in CAG through stages of hyperplasia and dysplasia involving both metaplastic and non-metaplastic glands. Molecular alterations, including replication error and p53 and APC gene mutation and aneuploidy have been found in some of these conditions, confirming their role in carcinogenesis. Carcinomas of the stomach are heterogeneous in cellular composition. Both intestinal and gastric types of cells are found in all types of tumors, indicating the unique characteristics of gastric cancer. Many molecular lesions have been found in gastric carcinomas. Basic changes involve replication errors, telomerase activity, and aberrant CD44 transcripts. Many other changes often show differences in the frequency of their occurrence between the two major histological types of gastric carcinoma: well differentiated versus poorly differentiated, or intestinal type versus diffuse type. The timing and frequency of these changes in the stomach differ from the timing and frequency in colonic carcinogeneis. Pathological evaluation remains reliable and meaningful, in basic research as well as clinical management. To obtain correlation with molecular alterations, the need for detailed pathologic classification of gastric carcinoma is recognized, taking into account its biologic behavior and grades of cell differentiation.The cellular and molecular pathology of gastric cancer and its precursors are reviewed and discussed. Gastric carcinomas are unique in their heterogeneity in both cellular composition and molecular changes.

show abstract

“…The nuclear area is enlarged [63], cell cycle time is lengthened, and the cell life span is shortened [64]. Carcinoembryonic antigen and occasionally, p21ras may be present [65].…”

Section: Precancerous Lesions: Epithelial Dysplasiamentioning

confidence: 99%

Cellular and molecular pathology of gastric carcinoma and precursor lesions: A critical review

Ming

1998

Gastric Cancer

View full text Add to dashboard Cite

show abstract

“…Hence, linear, measured area, estimated area and volume nuclear values, as well as nuclear cytoplasmic ratio have been found useful discriminators of malignant change. Examples are cervical carcinoma in situ (Foraker & Reagan 1956), thyroid cytology (Baak et al 1982a, Boon et al 1980), ovarian tumours (Baak et al 1981c, breast neoplasia (Bhattacharjee, Harris & Faragher 1985), endometrial cytology (Skaarland 1985) and gastric dysplasia and carcinoma (Jarvis & Whitehead 1985). Studies on colonic neoplasia have underlined the importance of nuclear cytoplasmic ratio (Nakamura et al 1974) and nuclear and nucleolar enlargement (Sato et al 1981).…”

Section: Discussionmentioning

confidence: 96%

Classification of normal colorectal mucosa and adenocarcinoma by morphometry

et al. 1987

View full text Add to dashboard Cite

Semi-automatic image analysis was used to make a morphometrical assessment of 15 nuclear and cellular variables in normal (n = 20) and malignant (n = 30) colorectal epithelium. Principal components analysis on the matrix of correlations between variables identified four main sources of variation within the dataset. These were, in decreasing order of importance: (1) nuclear size, nuclear cytoplasmic ratio and nuclear position within the cell; (2) the variability of nuclear size; (3) nuclear elongation and polarity; (4) nuclear shape and its variation. Discriminant analysis was conducted between histologically normal mucosa (n = 10) and adenocarcinoma in ulcerative colitis (n = 20). Using stepwise variable selection, the mean nuclear cytoplasmic ratio (normal, mean 20.4 (s.d. +/- 2.0); tumour, mean 39.7 (s.d. +/- 7.0)) and the coefficient of variation of nucleus to cell apex distance (normal, mean 19.2 (s.d. +/- 7.5); tumour, mean 47.8 (s.d. +/- 9.1)) were chosen as discriminating features. They were used to derive a discriminant function which gave perfect discrimination between the two groups. Scatter plots of these two variables confirmed complete separation of normal mucosa from adenocarcinoma and provided a simple method of applying the discriminant function. Discriminatory performance did not deteriorate when the function was applied to further normals (n = 10) and adenocarcinoma (n = 10). This study highlights the descriptive differences between normal and malignant colorectal epithelium and shows that case allocation may be made to these two lesion categories using a morphometrically-derived classification rule.

show abstract

“…The nuclear morphology of tumour cells, as seen by microscopy, is a very important indicator of cell behaviour, and quantitation of parameters such as DNA content [ 16], nuclear profile, and nuclear and nucleolar area [ 18,19,20], are potential methods for objective assessment of prognosis. In both breast [22,24,25] and prostatic cancer [13], morphometric analysis of nuclear parameters has been shown to correlate with metastatic potential and prognosis.…”

Section: Discussionmentioning

confidence: 99%

A morphometric study of invasion and metastasis in human colorectal carcinoma

Watson

Carr

1987

Clin Exp Metast

View full text Add to dashboard Cite

The cell nuclei within primary and nodal secondaries of colorectal carcinomas were analysed morphometrically in order to identify a primary subpopulation with similar characteristics to cells involved in lymphatic metastasis. No significant difference in nuclear area was seen between cell populations of the primary and secondary. However, a regional variation in mean nuclear area was present, suggesting a relationship between cells at the invasion front and in early nodal metastasis. This subpopulation differed from the cell populations of the primary and more advanced secondaries. These results are consistent with the existence of a morphometrically identifiable metastatic subpopulation within the invasion front of colorectal carcinoma.

show abstract

Morphometric analysis of gastric dysplasia

Cited by 35 publications

References 8 publications

Cellular and molecular pathology of gastric carcinoma and precursor lesions: A critical review

Cellular and molecular pathology of gastric carcinoma and precursor lesions: A critical review

Classification of normal colorectal mucosa and adenocarcinoma by morphometry

A morphometric study of invasion and metastasis in human colorectal carcinoma

Contact Info

Product

Resources

About