Morphometric Analysis of Rat Hepatocytes After Total Biliary Obstruction

Jones, Albert L.; Schmucker, Douglas L.; Mooney, Jill S.; Adler, Rivka; Ockner, Robert K.

doi:10.1016/s0016-5085(76)80057-1

Cited by 83 publications

(29 citation statements)

References 20 publications

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“…These data are in good agreement with those describing enhanced hepatic protein synthetic capacity in rats during the terminal portion of the life span. This study also confirmed the existence of a lobular gradient in the concentration of RER, i.e., the zone 1 hepatocytes contain slightly more of this membrane species than do centrolobular cells (Loud, 1968;Jones et al, 1976). Another quantitative morphological analysis mea- ANIMAL AGE (Months) Fig.…”

Section: Rough-surfaced Endoplasmic Reticulumsupporting

confidence: 73%

Hepatocyte fine structure during maturation and senescence

Schmucker

1990

J. Elec. Microsc. Tech.

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Aging is accompanied by a myriad of changes in cell structure, function, and composition. The fact that much of the information concerning age-related alterations in cellular morphology is qualitative precludes meaningful correlations with biochemical changes in order to enhance data interpretation. The mammalian liver has been subjected to both qualitative and quantitative evaluations of hepatocyte structure as a function of aging, i.e., development, maturation, and senescence. Although these data are characterized by considerable variability and, in some instances, blatant contradictions, there exists sufficient agreement in several parameters to permit a consensus in the inbred rat model. Certainly the volume of individual hepatocytes increases with age, and many of the organelle compartments reflect this change. While old rats exhibit a high incidence of polyploidy, there is no definitive evidence to demonstrate a concomitant increase in the binuclear hepatocyte index. Several specific hepatocellular organelles undergo changes in their relative volume or surface area that appear to correlate with functional alterations. The volume density of the lysosomal compartment enlarges significantly during senescence and is accompanied by increased activities of several constituent hydrolases. The hepatic concentration of smooth-surfaced endoplasmic reticulum declines markedly with aging, as does the yield of liver microsomes and the activities of several microsomal enzymes, e.g., mono-oxygenases and glucose-6-phosphatase. However, the responses of the majority of hepatocyte organelles to aging is varied and inconsistent based on the limited data currently available.

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Section: Rough-surfaced Endoplasmic Reticulumsupporting

confidence: 73%

Hepatocyte fine structure during maturation and senescence

Schmucker

1990

J. Elec. Microsc. Tech.

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“…After 2-3 days of cholestasis, dilation and proliferation of small intralobar and interlobular bile ducts occur (11). Approximately 7 days after obstruction, fibroblast proliferation and leukocyte infiltration start and are followed by fibrosis and bile duct proliferation (19,20). In our study on histopathologic examination of the liver for 7 days after bile duct ligation mitotic activity, Councilman bodies, portal inflammation, ductular proliferation and fibrosis were observed in the DMSO and control groups.…”

Section: Discussionmentioning

confidence: 50%

The Effects of Dimethylsulfoxide in Experimental Obstructive Jaundice

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Yılmaz

et al. 2003

Acta Chirurgica Belgica

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“…Multiple factors may be involved in the ability of some bile acid species to accelerate HRP output into bile both in the intact rat [13,24] and in the isolated perfused liver [12]. However, although short exposure to bile acids may stimulate the TransCP, maintained elevated levels of bile acids could inhibit the activity of microtubule-based motor proteins [25], resulting in the reported impairment of microtubule-dependent vesicular transport during cholestasis [26,27]. Similarly, owing to the continuous exposure to high levels of bile acids that occurs during foetal liver development in the MOCP group [1], a certain degree of chronic inhibition of motor mechanisms responsible for vesicle movement along microtubules may exist.…”

Section: Discussionmentioning

confidence: 99%

Effect of maternal obstructive cholestasis during pregnancy on the biliary transport of horseradish peroxidase in the rat offspring

et al. 2003

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MOCP (maternal obstructive cholestasis during pregnancy) induces a reversible impairment in bile formation in young rats born to these mothers. The aim of the present study was to gain information on the effects of MOCP on the maturation of pathways involved in protein secretion into bile in young (4-week-old) rats. The amount of hepatic alpha-tubulin and the structure of the microtubular network were apparently not affected by MOCP. HRP (horseradish peroxidase) was used as a model protein, and its secretion into bile after administration through the jugular vein was measured. In adult (8-week-old) rats, two peaks of HRP output into bile were observed following administration: an early peak presumably due to paracellular transfer, and a late peak presumably due to transcytosis. In young rats (4 weeks old), the early peak was similar to that of adult animals, and was not affected by MOCP. However, the late peak was markedly smaller in young control rats, and was further reduced by MOCP. Brefeldin A decreased, whereas taurocholate did not change, the early peak, whereas both affected the transcytotic transport of HRP. Brefeldin A delayed HRP secretion (similarly in control and MOCP groups), without affecting cumulative output, whereas taurocholate accelerated the transcytotic transport of HRP in the control group, but not in the MOCP group. These results suggest that MOCP affects the maturation of hepatocyte mechanisms involved in the transcytotic secretion of HRP into bile.

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Morphometric Analysis of Rat Hepatocytes After Total Biliary Obstruction

Cited by 83 publications

References 20 publications

Hepatocyte fine structure during maturation and senescence

Hepatocyte fine structure during maturation and senescence

The Effects of Dimethylsulfoxide in Experimental Obstructive Jaundice

Effect of maternal obstructive cholestasis during pregnancy on the biliary transport of horseradish peroxidase in the rat offspring

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