Morphometric analysis of somatotropic cells of the adenohypophysis and muscle fibers of the psoas muscle in the process of aging in humans

Antić, Vladimir; Stefanović, Natalija; Jovanović, Ivan; Antic, Milorad; Milić, Miroslav; Krstić, Miljan; Kundalić, Braca; Milošević, Verica

doi:10.1016/j.aanat.2015.01.006

Cited by 4 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in contrast to these authors, we could not confirm any significant decline of volume density of somatotropic cells with advancing age. Our study showed that the appropriate structural changes in somatotropic cells are responsible for the functional decline of the GH/IGF-1 axis during human aging, which is consistent with the earlier work of Antić et al [20]. The dynamics of these changes at the cellular level was influenced by numerous, insufficiently known, distant and local factors in the cell environment.…”

Section: Discussionsupporting

confidence: 92%

“…On the other hand, nuclear-cytoplasmic ratio declined uniformly with years of age, given that statistically significant differences were observed only between age groups I and III. The increase in size of somatotropes during ageing, as shown in this study, is in accordance with the research of Antić et al [20]. However, in contrast to these authors, we could not confirm any significant decline of volume density of somatotropic cells with advancing age.…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing

et al. 2022

View full text Add to dashboard Cite

Introduction/Objective. In this study, we have pointed out the immunohistomorphometric characteristics of somatotropic (GH) and folliculo-stellate (FS) cells of the human pituitary gland during ageing. Methods. On histological sections of the pituitary gland of 14 male cadavers of different ages, the GH and FS cells were immunohistochemically labeled with corresponding antibodies, monoclonal anti-GH antibody and polyclonal anti-S100 antibody, respectively. Immunopositive GH- and FS-cells were further morphometrically analyzed using ImageJ software. Results. The obtained results of morphometric analysis showed that the surface area of GH cells increased significantly with age. In these cells, the nuclear-cytoplasmic ratio gradually decreased and became significantly higher after the age of 70 years. The volume density of GH cells has not changed during ageing, while in FS cells this parameter significantly increased in the cases older than 70 years. The nuclear-cytoplasmic ratio of GH cells is negatively correlated with the volume density of FS cells. Conclusions. Based on the obtained results, we concluded that hypertrophy of GH and FS cells occurs in men with ageing and that correlation between the morphometric parameters of these two cell types indicates their mutual interaction.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Type II muscle fibers are usually larger in men, but type I muscle fibers are of equal or greater diameter in women. It was thought that the gender difference was the consequence of greater height and weight of men compared to women, with greater muscle mass and more physically active 26,27 . The impact of male sex hormones cannot be neglected either.…”

Section: Discussionmentioning

confidence: 99%

Comparative histochemical and morphometric analysis of muscle fibers of the psoas muscle in individuals of both genders with ageing

2022

View full text Add to dashboard Cite

Background/Aim. In this paper, we analyzed type I and type II muscle fibers of the iliopsoas muscle in persons of both genders with ageing. The aim of this study was to detect the presence and distribution of types I and II muscle fibers in the human psoas muscle using the hematoxylin and eosin method in individuals of different ages and genders. Methods. The material consisted of tissue samples of the right iliopsoas muscle taken from 30 adult cadavers (18 males and 12 females), aged from 30 to 90 years, divided into three age groups. The material was obtained from the Institute of Forensic Medicine, Faculty of Medicine University of Nis. Results. The values of astereological parameters (area, perimeter and Feret's diameter) of type I and type II muscle fibers were higher in male cases, although without any statistical significance. Conclusion. Based on the histochemical and morphometric analysis, the conclusion was drawn that after 70 years of life there occurred a loss of type II muscle fibers, more conspicuous in female cases.

show abstract

Histologic Investigation of the Salpingopharyngeus

Perta

Sewell

Cray

et al. 2022

J Speech Lang Hear Res

View full text Add to dashboard Cite

Purpose: The purpose of this study was to characterize the histologic contents of the salpingopharyngeal fold. The primary objective was to observe the presence of salpingopharyngeus (SP) muscle fibers, particularly at the section near the muscle origin at the torus tubarius (TT). Method: Histologic samples of the salpingopharyngeal fold from 10 cadavers (six females and four males) were analyzed. Following a head bisection, a tissue sample measuring 5 mm in length along the course of the salpingopharyngeal fold was collected from one side (i.e., right or left). The tissue sample was taken from the estimated base of the TT to a point 5 mm inferiorly. Slides were prepared using a standard histological approach and basic pathological staining and analyzed via bright-field microscopy. Results: Skeletal muscle fibers were identified in eight of the 10 tissue blocks of the salpingopharyngeal fold, with dense connective tissue identified in the remaining two tissue blocks. Glandular material was also identified in all 10 tissue blocks. Conclusions: Skeletal muscle fibers and/or dense connective tissue can be consistently identified in the section of the salpingopharyngeal fold near the TT. Glandular material is also consistently present in this same region of the salpingopharyngeal fold. These findings are discussed in relation to possible functional roles of the salpingopharyngeal fold contents, including the SP muscle.

show abstract

Morphometric analysis of somatotropic cells of the adenohypophysis and muscle fibers of the psoas muscle in the process of aging in humans

Cited by 4 publications

References 27 publications

Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing

Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing

Comparative histochemical and morphometric analysis of muscle fibers of the psoas muscle in individuals of both genders with ageing

Histologic Investigation of the Salpingopharyngeus

Contact Info

Product

Resources

About