Morphometric and Genetic Variability Among Tylenchulus semipenetrans Populations from Citrus Growing Area in Korea

Park, Byeong Yong; Park, Sun Nam; Lee, Jae Kook; Bae, Chang Hoon

doi:10.5423/ppj.2009.25.3.236

Cited by 10 publications

(12 citation statements)

References 3 publications

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“…Choi (2001) described a population of this species with slightly shorter male stylet (7-19 vs 10-12 µm) and spicules (17-22 vs 14-18 µm). Compared with the material examined byPark et al (2009), the Iranian population differs in PVSL (vs 23.6-44.5 µm) male stylet…”

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confidence: 93%

Distribution, morphology, seasonal dynamics, and molecular characterization of Tylenchulus semipenetrans from citrus orchards in southern Iran

et al. 2015

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Tylenchulus semipenetrans was identified morphologically from root samples collected from citrus orchards in Shahdad region, southeastern Iran. The number of juveniles and females averaged 648/100 g soil and 65/10 g root, respectively, in infected samples. Monitoring these orchards for two years (2012)(2013) indicated no significant correlation between soil pH (7.21-7.70) and EC (0.56-1.98) values and populations densities of T. semipenetrans. With respect to seasonal distribution, T. semipenetrans attained the highest density during fall and the lowest in spring. Sequence analysis of the D2-D3 segment of 28S rDNA confirmed this population as T. semipenetrans. Nblast indicates that the sequence from this population differs in only three or five nucleotides from corresponding sequences from T. semipenetrans populations from Korea (FJ969715, FJ060713, and FJ969712), with 99% identity. The phylogenetic position and a table of measurements of this species are provided.

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confidence: 93%

Distribution, morphology, seasonal dynamics, and molecular characterization of Tylenchulus semipenetrans from citrus orchards in southern Iran

et al. 2015

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“…The newly obtained sequences for each gene were aligned using ClustalX 1.83 (Thompson et al, 1997) with default parameters with their corresponding published gene sequences, ITS-rRNA or D2-3 of 28S rRNA, re-spectively (Subbotin et al, 2006;Park et al, 2009;Liu et al, 2011;Chen et al, unpubl.). Outgroup taxa for each dataset were chosen according to the results of previous published data (Subbotin et al, 2005(Subbotin et al, , 2006Bert et al, 2008;Holterman et al, 2009).…”

Section: Sequence and Phylogenetic Analysismentioning

confidence: 99%

“…Heterogeneity of the ITS-rRNA region for T. floridensis was revealed by enzymes BsuRI, BseNI and Hin6I. Wang et al (2004) and Park et al (2009) were the first to present diagnostic PCR-ITS-RFLP profiles for the citrus root nematode, T. semipenetrans, from China and Korea, respectively. Park et al (2009) revealed the heterogeneity of the ITS region after digestion by MseI and MspI and the presence of two ITS haplotypes.…”

Section: Molecular Diagnostics Of Tylenchulus Speciesmentioning

confidence: 99%

“…Tylenchulus graminis and T. palustris are often found together in some native areas in Florida. Wang et al (2004) and Park et al (2009) developed a diagnostic PCR-ITS-RFLP profile for T. semipenetrans and Liu et al (2011) were the first to propose speciesspecific primer sets for detection of this nematode. However, these primers were tested with a few citrus root nematode populations only.…”

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confidence: 99%

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Description of Tylenchulus musicola sp. n. (Nematoda: Tylenchulidae) from banana in Iran with molecular phylogeny and characterisation of species of Tylenchulus Cobb, 1913

Maafi

Amani

Stanley

et al. 2012

Nematol

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During a survey conducted on banana plantations in Sistan and Blouchestan province, south-east Iran, a new species of Tylenchulus was extracted from the soil and roots of banana plants. This species, named Tylenchulus musicola sp. n., is characterised by mature females having a swollen, hook-shaped body with a conical and elongate post-vulval portion ending in a round terminus, males having a weak stylet and a cylindrical and thick tail ending in a bluntly rounded and smooth terminus, and by second-stage juveniles having a slender body and a posterior body portion ending in a finely pointed or mucronate terminus. The results of glasshouse host tests indicated that the new species does not parasitise sugarcane ratoons or sour orange seedlings. Tylenchulus musicola sp. n. is distinguished from other known Tylenchulus species by the sequences of D2-D3 expansion segments of 28S rRNA and ITS rRNA genes. Phylogenetic relationships within Tylenchulus were reconstructed based on rRNA gene sequences using Bayesian inference. Diagnostic PCR-ITS-RFLP profiles are presented for T. musicola sp. n., T. furcus, T. graminis, T. palustris, T. semipenetrans and Trophotylenchulus floridensis. PCR with species-specific primers and genus-specific primer are tested and developed for rapid identification of five Tylenchulus species. An identification key to Tylenchulus species is provided.

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“…In particular, it is difficult to distinguish J2 of T. semipenetrans from many other plant-parasitic and non-plant-parasitic nematode species microscopically. As an alternative strategy for T. semipenetrans identification, molecular diagnostic methods based on polymerase chain reaction (PCR) have been developed and applied, including PCR-restriction fragment length polymorphism (RFLP) of the internal transcribed spacer region (ITS) (Maafi et al, 2012; Park et al, 2009) and PCR with species-specific primer sets designed from rDNA-ITS (Liu et al, 2011; Maafi et al, 2012). While PCR-based detection methods provided faster, more reliable and more sensitive tools for T. semipenetrans identification when compared with traditional morphological method, they are not generally available for routine practical applications at grassroots quarantine stations and plant protection organizations because expensive and sophisticated laboratory equipment and skilled technicians are needed.…”

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confidence: 99%

Development and Evaluation of Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Tylenchulus semipenetrans Using DNA Extracted from Soil

Song

Junfeng

Cheng

et al. 2017

The Plant Pathology Journal

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Tylenchulus semipenetrans is an important and widespread plant-parasitic nematode of citrus worldwide and can cause citrus slow decline disease leading to significant reduction in tree growth and yield. Rapid and accurate detection of T. semipenetrans in soil is important for the disease forecasting and management. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed to detect T. semipenetrans using DNA extracted from soil. A set of five primers was designed from the internal transcribed spacer region (ITS1) of rDNA, and was highly specific to T. semipenetrans. The LAMP reaction was performed at 63°C for 60 min. The LAMP product was visualized directly in one reaction tube by adding SYBR Green I. The detection limit of the LAMP assay was 10−2 J2/0.5 g of soil, which was 10 times more sensitive than conventional PCR (10−1 J2/0.5 g of soil). Examination of 24 field soil samples revealed that the LAMP assay was applicable to a range of soils infested naturally with T. semipenetrans, and the total assay time was less than 2.5 h. These results indicated that the developed LAMP assay is a simple, rapid, sensitive, specific and accurate technique for detection of T. semipenetrans in field soil, and contributes to the effective management of citrus slow decline disease.

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Morphometric and Genetic Variability Among Tylenchulus semipenetrans Populations from Citrus Growing Area in Korea

Cited by 10 publications

References 3 publications

Distribution, morphology, seasonal dynamics, and molecular characterization of Tylenchulus semipenetrans from citrus orchards in southern Iran

Distribution, morphology, seasonal dynamics, and molecular characterization of Tylenchulus semipenetrans from citrus orchards in southern Iran

Description of Tylenchulus musicola sp. n. (Nematoda: Tylenchulidae) from banana in Iran with molecular phylogeny and characterisation of species of Tylenchulus Cobb, 1913

Development and Evaluation of Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Tylenchulus semipenetrans Using DNA Extracted from Soil

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