Morphometric and ISSR-Analysis of Local Populations of Geranium molle L. from the Southern Coast of the Caspian Sea

Esfandani‐Bozchaloyi, Somayeh; Sheidai, Masoud; Keshavarzi, Maryam; Noormohammadi, Zahra

doi:10.3103/s0095452718040102

Cited by 35 publications

(27 citation statements)

References 33 publications

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“…Geography appears to play an important role in isolation by distance, particularly for Mediterranean plants. Reductions in gene flow may lead to the appearance of new species or subspecies, with isolation in glacial refugia as a major promoter of such diversification (ESFANDANI-BOZCHALOYI et al, 2018a;2018b;2018c;2018d). E. cicutarium is of wide spread in our country and it has several medicinal applications (WIESNEROVA and WIESNER, 2004), however we had no information on its genetic structure and detailed taxonomic information.…”

Section: Discussionmentioning

confidence: 99%

Population differentiation and gene flow in Rrodium cicutarium: A potential medicinal plant

2020

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Erodium (Geranaiceae) species are distributed in different habitats of Iran. Some species are of medicinal importance while some are well known weeds. In arid and semi-arid regions, E. cicutarium has had some importance as a forage plant and is an important grazing plant and source of protein supplements to straw for ruminants in semi deserts and wastelands of the Middle East. There is no information on its population genetic structure, genetic diversity, and morphological variability in Iran. Due to the medicinal importance of this species, a genetic variability and populations? structure study is performed studying 15 geographical populations of E. cicutarium Therefore, we used six inter-retrotransposon amplified polymorphism (IRAP) markers and 15 combined IRAP markers to reveal within and among population genetic diversity in this plant. AMOVA test produced significant genetic difference (PhiPT = 0.39, P = 0.010) among the studied populations and also revealed that, 55% of total genetic variability was due to within population diversity while, 45% was due to among population genetic differentiation. Mantel test showed positive significant correlation between genetic distance and geographical distance of the studied populations. Networking, STRUCTURE analyses and population assignment test revealed some degree of gene flow among these populations. PCoA plot of populations based on morphological characters was in agreement with MDS plot of molecular data. These results indicated that geographical populations of E. cicutarium are well differentiated both in genetic content as well as morphological characteristics. Consensus tree based on morphological and genetic data separated some of these populations from the others suggesting the existence of ecotypes within this species.

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Section: Discussionmentioning

confidence: 99%

Population differentiation and gene flow in Rrodium cicutarium: A potential medicinal plant

2020

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“…RAPD analysis using PCR along with short arbitrary sequence primers has been reported sensitive to detecting variation at level of individuals. The benefits of this method are: a) a large number of samples are tested easily and efficiently using less quantity of material; b) the DNA amplicons are independent of ontogenetic expression; c) several genomic regions may be sampled with likely infinite numbers of markers (SONIYA et al 2001;ESFANDANI-BOZCHALOYI et al, 2018 a;2018b;2018c;2018d).…”

Section: Introductionmentioning

confidence: 99%

RAPD profiling in detecting genetic variation in Stellaria L. (Caryophyllaceae)

2021

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Stellaria species are common herbs, preferred humid mountainously slopes, but some grew in desert. Main center of diversification for Stellaria is Eurasia, with a center of distribution in the mountains of central Asia. Some species are also cosmopolitan. It is represented by 9 species in Iran. The genus has high medicinal value. To determine the genetic diversity and understand the species? limits within the Iranian Stellaria, we produced molecular data using 139 randomly collected plants representing 8 species from five provinces of Iran. A total of 122 reproducible bands were generated by 10 of 25 random amplified polymorphic DNA (RAPD) primers, with an average of 12.2 bands/primer and 33% polymorphism. Largest number of effective alleles (Ne), genetic diversity (H), and Shannon Index (I) were shown by S. media. Our data depicted highest similarity between S. media and S. pallida and lowest between S. media and S. graminea. S. pallida showed relatively low level of genetic variation. Finally, the Neighbor Joining (NJ) trees based on RAPD markers data divided the populations into two different clusters, indicating their genetic difference which is discussed in details.

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“…They can reveal the pattern of gene flow between closely related phylogenetic species versus isolation by distance and identify the evolutionary process by which new biological species arise (Freeland et al 2011). Species delimitation is a difficult task, particularly in species with cross-pollination breeding systems that tend to form frequent inter-specific hybrids (Esfandani Bozchaloyi et al 2017b, 2018a, 2018b.…”

Section: Introductionmentioning

confidence: 99%

Molecular diversity and genetic relationships amongGeranium pusillumandG. pyrenaicumwith inter simple sequence repeat (ISSR) regions

Bozchaloyi

Sheidai

2018

Caryologia

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Successful management and preservation of natural populations depend on accurate assessment of genetic diversity. Knowing the genetic diversity within a population is important for choosing the conservation strategies for the species. Geranium pusillum L. is an annual or biennial herb mainly native to Europe, northern Africa, and western Asia, and G. pyrenaicum Burm is a frost hardy perennial native to areas of France and Spain to the Caucasus. In general, taxonomic and biosystematic studies of Geranium are not known in Iran; moreover, in a few cases, inter-specific hybrids and intermediate forms are recognized. A detailed morphological, micro-morphological and molecular (ISSR) study of Geranium is done here with the following objectives: (1) to delimitate the species; (2) to carry out a population genetic study and produce information on genetic structure and genetic variability within each population in G. pyrenaicum and G. pusillum. The present study revealed that a combination of morphological and micro-morphological (seed and pollen) data can separate the species. In the present study, 84 randomly collected plants from 12 geographical populations of two Geranium species were considered. A consensus tree based on morphological and genetic data separated some of these populations from the others, suggesting the existence of ecotypes in the studied taxa.

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Morphometric and ISSR-Analysis of Local Populations of Geranium molle L. from the Southern Coast of the Caspian Sea

Cited by 35 publications

References 33 publications

Population differentiation and gene flow in Rrodium cicutarium: A potential medicinal plant

Population differentiation and gene flow in Rrodium cicutarium: A potential medicinal plant

RAPD profiling in detecting genetic variation in Stellaria L. (Caryophyllaceae)

Molecular diversity and genetic relationships amongGeranium pusillumandG. pyrenaicumwith inter simple sequence repeat (ISSR) regions

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