Morphometric differences in sperm head dimensions of fertile and subfertile stallions

Casey, Patrick J.; Gravance, Curtis G.; Davis, Russell O.; Chabot, D.D.; Liu, I.K.M.

doi:10.1016/s0093-691x(97)00015-0

Cited by 93 publications

(62 citation statements)

References 24 publications

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“…It has been found that semen containing spermatozoa with head defects may reduce the quality of embryos (De Jarnette et al 1992) and results in miscarriages in the first months of pregnancy (Chenoweth 2005). Larger sperm heads determined in the semen of stallions with decreased fertility may indicate disturbed spermatogenesis, and, first and foremost, changes in the chromatin structure taking place during sperm maturation and transportation in the duct of epididymis (Casey et al 1997).…”

Section: Discussionmentioning

confidence: 99%

“…Boar prolificacy depends on the quality of generated reproductive cells which can be determined by examining sperm morphology. The results of such an examination make it possible to check if a male is fertile or not (Soderquist et al 1991, Casey et al 1997, De Vos et al 2003, Philips et al 2004. A high frequency of spermatozoa with morphological abnormalities is an important indicator Correspondence to: S. Kondracki, e-mail: sk@uph.edu.pl, tel.…”

Section: Introductionmentioning

confidence: 99%

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Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars

Kondracki¹,

Wysokińska²,

Iwanina³

et al. 2011

Polish Journal of Veterinary Sciences

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The experimental material consisted of 75 ejaculates collected form 8 Duroc boars. The ejaculates were divided into three groups according to sperm concentration in an ejaculate. An ejaculate was obtained from each boar monthly and it was used to make microscopic preparations to examine spermatozoa morphology. In each preparation morphometric measurements were taken of fifteen randomly selected spermatozoa characterized by normal morphology. The following measurements of spermatozoa were taken: length and width of the spermatozoa head, head area, length of the flagellum, perimeter of the spermatozoon head and total spermatozoon length. The results were used to calculate indicators of spermatozoa morphology. Moreover, assessments were made of frequency of morphological defects to isolate spermatozoa with primary and secondary abnormalities following the Blom classification system. It was found that the concentration of spermatozoa in the ejaculate influenced the morphometric characteristics of spermatozoa. Ejaculates with low sperm concentrations are characterized by larger spermatozoa as compared to ejaculates with high sperm concentrations. However, sperm concentration in the ejaculate does not much influence the shape of spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars

Kondracki¹,

Wysokińska²,

Iwanina³

et al. 2011

Polish Journal of Veterinary Sciences

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“…In this context determination of the normality of sperm head size and shape becomes particularly important, as they are important criteria in classifying sperm as having normal or abnormal morphological structure. A number of studies have shown that the average size of sperm heads in semen with abnormal morphology is larger than in reproductive cells from normal ejaculates (7).…”

Section: Introductionmentioning

confidence: 99%

Morphometric dimensions of the stallion sperm head depending on the staining method used

Banaszewska¹,

Czubaszek

Walczak-Jędrzejowska

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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Semen was collected from Polish Halfbred stallions. Twenty individuals from 3 to 4 years of age were selected for the study. At least one ejaculate from each stallion was collected and assessed. Sperm morphology was evaluated using Papanicolaou stain, SpermBlue ® , and a complex of eosin and gentian stain, whereas unstained slides were used as control samples. Morphometric measurements were performed on 100 randomly selected sperm heads in each ejaculate. The length, width, perimeter, and surface area of the sperm head were measured. The frequency of nuclear vacuoles was determined as well. Tygerberg's strict criteria, which most precisely characterise the sperm head, were used in the morphological evaluation of the sperm. The results obtained indicate that in the case of staining with SpermBlue ® and with eosin+gentian complex, the morphometry of the sperm head seems to be most similar to that observed in unstained smears. It also seems that neither shrinking nor swelling is uniform in the various staining techniques (Papanicolaou stain). Moreover, it appears that in comparison with unstained sperm, all methods caused the width of the head to increase as a result of swelling.

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“…The number and quality of his prodigy depend, however, on the quality of his spermatozoa. Morphological features of the spermatozoa provide useful criterion for assessing the quality of semen and clinical value for informing veterinary decisions [6]. Jasko et al reported that the rate of spermatozoa with normal morphology correlates positively with the rate of fertility [16].…”

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confidence: 99%

Spermatozoa Morphology during the Breeding Season in Thoroughbred Stallions in Japan

Koyago

Nakada

Tsunoda

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. The morphology of spermatozoa of modern Thoroughbred stallions in Japan was investigated during the breeding season. A total of 299 semen samples were collected from the penises of 16 stallions immediately after service. The rate of abnormalities in sperm heads and tails, spermatozoa with cytoplasmic droplets and slides with medusa cells to total observed slides in each stallion were 3.9 ± 2.1%, 11.5 ± 5.9%, 2.4 ± 2.6% and 20.1%, respectively. The values for the area, length, width and aspect ratio of the stallion sperm head were 12.54 ± The breeding stallion for Thoroughbred racehorses is prized for the chance of siring future champions. The number and quality of his prodigy depend, however, on the quality of his spermatozoa. Morphological features of the spermatozoa provide useful criterion for assessing the quality of semen and clinical value for informing veterinary decisions [6]. Jasko et al. reported that the rate of spermatozoa with normal morphology correlates positively with the rate of fertility [16]. Wide variation in the size and shape of spermatozoa occurs in virtually all mammalian species, however, and an abundance of abnormalities can be found. The sperm head has been reported to be larger in semen taken from subfertile stallions than in that from fertile stallions [6,14]. The converse has also been reported [17], and variations in size of the sperm head have been attributed to the staining methods of the studies [4,5,15,22]. The staining method has also influenced the ratio of morphologically abnormal spermatozoa to the total sperm count [18,21]. These problems remain unsolved.For a popular stallion, the number of services may exceed 200 in a single breeding season. A previous study showed in Thoroughbreds engaged in five services every hour that, in spite of decreases in volume, number or concentration of spermatozoa, sperm viability was not different in the ejaculation repeats [23].The morphology of the spermatozoa has not been investigated in Thoroughbred stallions during the breeding season in Japan. We hypothesized that if the ejaculated semen could be examined from time to time throughout the breeding season, the semen would exhibit morphological differences and allow clarification of abnormalities in the spermatozoa produced during that critical period in the work of the stallions. Morphological data on semen ejaculated throughout the breeding season would provide a reference index for judging the normal morphology of spermatozoa to estimate reproductive activity at copulation. To clarify the morphology of spermatozoa from Thoroughbred stallions servicing a large number of mares during the breeding season, in the present study we examined spermatozoa collected from the semen remaining in the urethra of the penis immediately after the stallion dismounted from servicing since sperm head measurements from dismount semen are representative of those of the ejaculate [13].The study was comprised 16 Thoroughbred stallions in Hokkaido within age range of 4 to 15 (mean ± SD, 8.9 ± 3.2) y...

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Morphometric differences in sperm head dimensions of fertile and subfertile stallions

Cited by 93 publications

References 24 publications

Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars

Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars

Morphometric dimensions of the stallion sperm head depending on the staining method used

Spermatozoa Morphology during the Breeding Season in Thoroughbred Stallions in Japan

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