Morphometric Features and Glycoconjugate Pattern of Rabbit Intestine are Affected by Particle Size of Pelleted Diets

Desantis, Salvatore; Zizza, Sara; Accogli, Gianluca; Tufarelli, Vincenzo; Laudadio, Vito

doi:10.1002/ar.21477

Cited by 6 publications

(6 citation statements)

References 82 publications

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“…Based on our results, we can also say that the addition of different types of fat does not affect the histological structure of the digestive tract, which is probably more dependent also on the fiber and particle size of the feed. Desantis, et al [47] reported that rabbits fed with more finely milled particles (2 mm) in feed had irregularly shaped and significantly longer villi in the duodenum compared to rabbits, of which particles in the feed were roughly ground (8 mm). Beside the longer villi, rabbits fed with finely milled feed also had deeper crypts in the duodenum and caecum, and were significantly deeper in the colon [47]; short intensive feed restriction increased villi height and crypt depth [48].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Substitution of Palm Fat with Linseed Oil on the Lipid Peroxidation, Antioxidative Capacity and Intestinal Morphology in Rabbits (Oryctolagus cuniculus)

Trebušak

Vrecl

Salobir

et al. 2019

Animals

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Simple SummaryBy changing the source of fat in the diet of animals, the fat content of animal products, such as meat, could be improved. The addition of linseed oil, with a high proportion of polyunsaturated fatty acids, especially n-3 α-linolenic acid, is often used in animal nutrition. Rabbit diet manipulation is effective in increasing or changing the level of fatty acids such that meat could become a functional food for humans. Since polyunsaturated fatty acids are susceptible on oxidation, an effect on animal health could arise. Therefore, we conducted a trial replacing palm fat (saturated fatty acids) with linseed oil (polyunsaturated fatty acids) in the rabbit diet to compere the oxidative status and histology of intestinal tissues. There was no significant effect in the substitution of linseed oil on the malondialdehyde concentration in urine and plasma, antioxidative capacity of water soluble or lipid soluble antioxidants, or intestinal morphology. Therefore, with an appropriate dietary strategy, the content of rabbit meat can be improved without reducing animal welfare.AbstractThis experiment was conducted to investigate the effect of different dietary fatty acids (saturated or polyunsaturated fatty acids) supplementation on the oxidative status and intestinal morphology of adult rabbits (Oryctolagus cuniculus). Twenty-four “slovenska kunka” rabbits were randomly assigned to two different dietary treatments (12 rabbits per treatment) and fed the experimental diets between 80 and 102 days of age. The palm fat (PALM) diet with 6% palm fat and linseed (LINSEED) diets with 6% linseed oil were used. To evaluate the oxidative status of rabbits, the malondialdehyde concentration in urine and plasma and concentration of water and lipid soluble antioxidants in plasma were measured. The antioxidative capacity of the gastrointestinal tract was evaluated by measuring concentration of water and lipid soluble antioxidants in tissues and contents of the intestine. The histological structure of the small intestine and caecum was analyzed via histomorphometric analysis. No significant differences were found in either of those parameters. In summary, rabbits were exposed to high levels of polyunsaturated fatty acids with a high predisposition to oxidation, but their health and welfare were not endangered.

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Section: Discussionmentioning

confidence: 99%

The Effect of Substitution of Palm Fat with Linseed Oil on the Lipid Peroxidation, Antioxidative Capacity and Intestinal Morphology in Rabbits (Oryctolagus cuniculus)

Trebušak

Vrecl

Salobir

et al. 2019

Animals

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“…Romero et al [ 11 ] also reported that finely ground (1.5 mm), dehydrated alfalfa increased the villous height and decreased the crypt of jejunum when compared with grinding at 4.5 mm. Desantis et al [ 46 ] reported that rabbits fed fine particles (2 mm) displayed more irregularly shaped, greater duodenal villi and deeper crypts in the distal colon as well as a greater number of goblet cells than rabbits fed coarse (8 mm) particles. The superfine grinding of fibers (100 and 10 μm) had a somewhat detrimental effect on small intestine morphology in present study.…”

Section: Discussionmentioning

confidence: 99%

Effects of the particle of ground alfalfa hay on the growth performance, methane production and archaeal populations of rabbits

et al. 2018

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The world's annual output of rabbits is over 1.2 billion, therefore this sector is also one of the sources of greenhouse gases in livestock production. One hundred-twenty New Zealand rabbits were allocated into four treatments, five replicates in each treatment and six rabbits in each replicate to examine the effect of grinding alfalfa hay to different sizes on growth performance, methane production and cecal archaeal populations. The particle sizes of the alfalfa meal in the four treatment diets were 2500, 1000, 100 and 10 μm, while the other ingredients were ground through a 2.5 mm sieve. The average daily gain (ADG) and average daily feed intake (ADFI) increased (P<0.001) as the particle size decreased, but the feed conversion ratio (FCR) was not affected (P = 0.305). The digestibility of neutral detergent fiber (NDF) (P = 0.006) and acid detergent fiber (ADF) (P<0.006) increased while the greatest digestibility of crude protein (CP) was obtained in 1000 um group (P = 0.015). The rabbits produced more methane (CH4, L/kgBM0.75/d) with decreasing alfalfa particle size (P<0.001). The molar proportion of acetic acid and propionic acid decreased (P<0.001) at the cost of butyric acid (P<0.001). The greatest villus height:crypt depth ratio were obtained in 1000 μm group, and the decrease in the alfalfa hay particle size decreased the jejunum and ilem villus height:crypt depth ratio (P<0.05). The gastric muscular and mucosal thickness decreased with decreasing alfalfa particle size (P<0.05). Archaea diversity decreased with decreasing alfalfa particle size, and the relative abundance of genus Methanobrevibacter increased (P<0.001) while the genus Methanosphaera decreased (P<0.001). It is concluded that a finer particle size favors the growth of genus Methanobrevibacter, which produces more methane but promotes the growth performance of rabbits.

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“…Lectin histochemistry, when combined with enzymatic digestion and chemical treatment, can be a valuable tool to highlight changes in the glycosylation of intestinal mucins (Desantis et al, 2011) and thus is valuable for diagnosis of diseases. The mouse is frequently used as animal model to investigate the mechanisms of development of intestinal disease induced by supply of drugs, diets, and genetic modification (Erdelyi et al, 2009;Fu et al, 2011;An et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

In situ characterization of O-linked glycans of Muc2 in mouse colon

et al. 2012

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Morphometric Features and Glycoconjugate Pattern of Rabbit Intestine are Affected by Particle Size of Pelleted Diets

Cited by 6 publications

References 82 publications

The Effect of Substitution of Palm Fat with Linseed Oil on the Lipid Peroxidation, Antioxidative Capacity and Intestinal Morphology in Rabbits (Oryctolagus cuniculus)

The Effect of Substitution of Palm Fat with Linseed Oil on the Lipid Peroxidation, Antioxidative Capacity and Intestinal Morphology in Rabbits (Oryctolagus cuniculus)

Effects of the particle of ground alfalfa hay on the growth performance, methane production and archaeal populations of rabbits

In situ characterization of O-linked glycans of Muc2 in mouse colon

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