Morphometric identification of equid cyathostome (Nematoda: Cyathostominae) infective larvae

Kornaś, Sławomir; Gawor, Jakub; Cabaret, Jacques; Molenda, Krzysztof; Skalska, M.; Nowosad, B.

doi:10.1016/j.vetpar.2009.03.018

Cited by 19 publications

(15 citation statements)

References 18 publications

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“…The number of larvae per gram (LPG) was obtained by multiplying the number of the counted larvae by a factor of ten . The strongyle species/families have been identified based on the third larval stage (L3) morphology , Kornaś et al 2009). Depending on the number of larvae in each sample, between 5-100 larvae per sample were identified and the results were expressed as a percentage.…”

Section: Species-specific Identification Of Equid Strongyles Based Onmentioning

confidence: 99%

Screening for anthelmintic resistance in equid strongyles (Nematoda) in Romania

Cernea

Cristina

Ștefănuț

et al. 2015

Folia Parasitologica

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Resistance to anthelmintic medication of equid strongyles is a worldwide phenomenon and for this reason systematic investigations of resistant parasite populations are necessary. The purpose of the present study was to investigate the presence and distribution of equid strongyles resistant to the anthelmintics used in Romania, as well as the pre-treatment and post-treatment prevalence of species of strongylid nematodes. The Faecal Egg Count Reduction Test was performed between 2010 and 2013 on a total number of 588 horses and 23 donkeys from 26 locations (subgroups). Animals of the first group (I) consisting of subgroups no. 1-11 were treated with Albendazole (ABZ), those of the second group (II) consisting of subgroups no. 12-23 with Fenbendazole (FBZ), while Ivermectin (IVM) was used on animals of the third group (III) consisting of subgroups no. 24-26. Resistant strongyles have been found in 82% (average lower limit of the 95% confidence interval, LCL95%, was 65) of the total equids from the group treated with ABZ. In the group of horses treated with FBZ, resistant strongyles were identified in 75% of the subgroups (LCL95% = 44). No resistant strongyles have been identified in IVM-treated horse groups (LCL95% = 98). The pre-treatment prevalence of the species of the Strongylinae Müller, 1780 was 22%, whereas that with nematodes of the subfamily Cyathostominae Molin, 1861 78%. Post-treatment reduction of strongyline nematodes was observed (5%), which demonstrates the sensitivity of large strongyles to common anthelmintics. The post-treatment prevalence of cyathostomes was of 95%, which proves their resistance, especially to ABZ- and FBZ-based anthelmintics.

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Section: Species-specific Identification Of Equid Strongyles Based Onmentioning

confidence: 99%

Screening for anthelmintic resistance in equid strongyles (Nematoda) in Romania

Cernea

Cristina

Ștefănuț

et al. 2015

Folia Parasitologica

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“…The morphological identification of L3 was made based on the following criteria: type of esophagus, intestinal cells (number, shape, and arrangement), and length of tail (Kornas et al, 2009 S. vulgaris larvae ( Fig. 1) were identified based on the following characteristics: long dimension (900 -1110 µm), short esophagus, long tail, and 28 -32 distinct intestinal cells .…”

Section: Resultsmentioning

confidence: 99%

“…For each larval culture, 1 ml of sediment was collected, centrifuged at 3000 rpm for 5 minutes, and analyzed according to the protocol described by Schneider et al (2014), as follows: 100 μl aliquot was used for counting and morphological identification and differentiation of larvae (larvae were immobilized with Lugol's iodine); the remaining sediment (900 μl) was analyzed for identification of S. vulgaris larvae. The strongyle larvae were identified using morphological keys, based on: number, shape, and arrangement of the intestinal cells, type of esophagus, tail length Kornas et al, 2009). …”

Section: Fecal Analyses Larval Culturing and Identification Of The mentioning

confidence: 99%

Investigating the Strongyle Populations, with Emphasis on Strongylus vulgaris (Nematoda: Strongylidae) in Romanian Horses, Based on Larval Cultures

Buzatu¹,

Mitrea²,

Gruianu³

et al. 2017

BUASVMCN-VM

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This study aimed to investigate the presence of Strongylus vulgaris on different horse establishments in Romania. A total number of 380 horses were enrolled in the study, representing: three stud farms (n=156); four sport/recreational establishments (n=75); and working horses (n=149). Overall, 106 premises/yards from 17 localities in 9 counties from North-Eastern, Center and Southern Romania were visited. Fresh fecal sample were collected from individual horses and were analyzed by a modified McMaster method for fecal strongyle egg per gram counting. Further, larval cultures were performed for morphological identification of S. vulgaris third stage larvae. Overall, of the investigated horses from studs, sport/recreational units, and working horses, 84.60%, 44.0%, and 79.20%, respectively were positive for strongyle. On coprocultures, cyathostomin-larvae were the most prevalent in all horse-establishment types, while S. vulgaris larvae were found only in working horses (12.1%; n=18). Other strongylin species have been identified, namely S. equinus, S. edentatus, Oesophagodontus robustus, and Triodontophorus spp. all in working horses, and Craterostomum acuticaudatum, in both working horses and horses for sport units. However, further studies are planned by using more sensitive technique for monitoring of S. vulgaris in Romanian horse farms.

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“…Some researchers developed morphometric criteria (Kornaś et al 2009) or molecular methods (Hodgkinson et al 2005;Traversa et al 2007) for cyathostome L 3 identification; however, the most practical certainty in identification of small strongyles is from specimens in the large intestine. This is important for determining the prepatent period of the numerous species of small strongyles.…”

Section: Discussionmentioning

confidence: 99%

Observations on development of natural infection and species composition of small strongyles in young equids in Kentucky

et al. 2011

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Early development and maturation of species of small strongyles have not been studied extensively. Most information is on the first appearance of strongyle eggs in feces of foals. However, species cannot be determined in this manner because of similarity of the morphological features of the eggs. To determine more definitive knowledge on development and species composition of natural infections of small strongyles, eight equid foals were necropsied and examined at various ages (31 to 92 days of age). The entire contents of the large intestine were examined, and all worms (6,671) recovered were identified. This was done to recover small strongyle specimens in an effort to find the stage of development of the worms for different time periods. The primary interest was to obtain data on the potential prepatent period. A total of 17 species of small strongyles were recovered. The earliest gravid female was Cylicostephanus longibursatus in a 57-day-old foal; then Cylicostephanus goldi was positive at 64 days, Cyathostomum catinatum at 68 days, Coronocyclus coronatus and Cylicostephanus calicatus at 70 days, Coronocyclus labiatus, Cylicocyclus leptostomus, Cylicocyclus nassatus, Cylicostephanus hybridus, and Cylicostephanus minutus at 74 days, and Cylicostephanus bidentatus at 92 days. While it is not known exactly when the foals began eating pasture vegetation (typically they begin to graze a short time after birth) and first ate infective free-living third-stage larvae, the results give an indication of the natural prepatent period of several species of small strongyles. Additional data are presented on the number of worms per foal, distribution of the worms in the parts (cecum, ventral colon, and dorsal colon) of the large intestine, and proportion of species found.

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Morphometric identification of equid cyathostome (Nematoda: Cyathostominae) infective larvae

Cited by 19 publications

References 18 publications

Screening for anthelmintic resistance in equid strongyles (Nematoda) in Romania

Screening for anthelmintic resistance in equid strongyles (Nematoda) in Romania

Investigating the Strongyle Populations, with Emphasis on Strongylus vulgaris (Nematoda: Strongylidae) in Romanian Horses, Based on Larval Cultures

Observations on development of natural infection and species composition of small strongyles in young equids in Kentucky

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