Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish eleutheroembryos

Martínez, Rubén; Herrero-Nogareda, Laia; Antro, Morgane Van; Campos, Maria Pilar; Casado, Marta; Barata, Carlos; Piña, Benjamı́n; Navarro‐Martín, Laia

doi:10.1016/j.aquatox.2019.105232

Cited by 29 publications

(31 citation statements)

References 127 publications

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“…To facilitate this process, we conducted a meta-analysis of existing literature to quantify the effects of BPA on aquatic organisms. A meta-analytic approach is important particularly because the effects of BPA on biological functions can be context dependent, and different taxa and biological traits show different responses when exposed to BPA (Abdel-Tawwab & Hamed, 2018;Martínez et al, 2019;Staples, Woodburn, Caspers, Hall, & Klecka, 2002;Wright-Walters, Volz, Talbott, & Davis, 2011). Given the ongoing development of policies regarding the environmental consequences of EDCs (Canesi & Fabbri, 2015;Crain et al, 2007;Flint, Markle, Thompson, & Wallace, 2012), there is an urgent need to evaluate environmental concentrations and their impact on aquatic organisms.…”

Section: Introductionmentioning

confidence: 99%

Effect of the plastic pollutant bisphenol A on the biology of aquatic organisms: A meta‐analysis

Seebacher

2020

Global Change Biology

111

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Plastic pollution is a global environmental concern. In particular, the endocrine‐disrupting chemical bisphenol A (BPA) is nearly ubiquitous in aquatic environments globally, and it continues to be produced and released into the environment in large quantities. BPA disrupts hormone signalling and can thereby have far‐reaching physiological and ecological consequences. However, it is not clear whether BPA has consistent effects across biological traits and phylogenetic groups. Hence, the aim of this study was to establish the current state of knowledge of the effect of BPA in aquatic organisms. We show that overall BPA exposure affected aquatic organisms negatively. It increased abnormalities, altered behaviour and had negative effects on the cardiovascular system, development, growth and survival. Early life stages were the most sensitive to BPA exposure in invertebrates and vertebrates, and invertebrates and amphibians seem to be particularly affected. These data provide a context for management efforts in the face of increasing plastic pollution. However, data availability is highly biased with respect to taxonomic groups and traits studies, and in the geographical distribution of sample collection. The latter is the case for both measurements of the biological responses and assessing pollution levels in water ways. Future research effort should be directed towards biological systems, such as studying endocrine disruption directly, and geographical areas (particularly in Africa and Asia) which we identify to be currently undersampled.

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Section: Introductionmentioning

confidence: 99%

Effect of the plastic pollutant bisphenol A on the biology of aquatic organisms: A meta‐analysis

Seebacher

2020

Global Change Biology

111

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“…The morphometric analysis ( Figure 1 ) showed that LIN did not affect the morphometric measures, while the PM—and above all, the combined LIN-PM treatment—influenced the HW and IOD of zebrafish larvae, highlighting the ability of these chemicals to affect the craniofacial features of exposed zebrafish. IOD was also reduced following exposure to endocrine disruptors able to negatively impact the proper development of the brain structure of developing zebrafish larvae (e.g., bisphenol A and PFOS) [ 37 ]. Combined exposure of LIN and PM exerted more pronounced and significant effects on the reduction of head width compared to single pesticide exposure.…”

Section: Discussionmentioning

confidence: 99%

An Experimental Approach to Study the Effects of Realistic Environmental Mixture of Linuron and Propamocarb on Zebrafish Synaptogenesis

Caioni

Merola

Perugini

et al. 2021

IJERPH

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The reasons behind the extensive use of pesticides include the need to destroy vector organisms and promote agricultural production in order to sustain population growth. Exposure to pesticides is principally occupational, even if their persistence in soil, surface water and food brings the risk closer to the general population, hence the demand for risk assessment, since these compounds exist not only as individual chemicals but also in form of mixtures. In light of this, zebrafish represents a suitable model for the evaluation of toxicological effects. Here, zebrafish embryos were exposed for 96 h post fertilization (hpf) to sublethal concentrations (350 µg/L) of linuron and propamocarb, used separately and then combined in a single solution. We investigated the effects on morphological traits and the expression of genes known to be implicated in synaptogenesis (neurexin1a and neuroligin3b). We observed alterations in some phenotypic parameters, such as head width and interocular distance, that showed a significant reduction (p < 0.05) for the mixture treatment. After individual exposure, the analysis of gene expression showed an imbalance at the synaptic level, which was partially recovered by the simultaneous administration of linuron and propamocarb. This preliminary study demonstrates that the combined substances were responsible for some unpredictable effects, diverging from the effect observed after single exposure. Thus, it is clear that risk assessment should be performed not only on single pesticides but also on their mixtures, the toxicological dynamics of which can be totally unpredictable.

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“…During the exposure period, fresh experimental solutions were prepared every J o u r n a l P r e -p r o o f day by dilution of the stock solutions in fish water (final DMSO concentration: 0.2% (v/v)). With the aim to avoid possible molecular events directly related with cellular/organism death processes, the exposure was set up using as a maximum TBT concentration the previously reported lowest observed adverse effect concentration (LOAEC) in terms of survival, hatching or swim bladder inflation rates (100 nM) (Martínez et al, 2019a). Based on that, we chose to expose zebrafish eleutheroembryos to nominal concentrations of 0 (control; 0.2% DMSO), 3, 30 and 100 nM of TBT from 2 to 5 dpf.…”

Section: Zebrafish Eleutheroembryos Exposures To Tbtmentioning

confidence: 99%

“…Exposures were carried out from 2 to 5 dpf to ensure that many of the endocrine systems were developed or under development (Löhr and Hammerschmidt, 2011) and also to avoid toxic effects at early embryonic developmental processes to focus the analysis on already differentiated cells and tissues. This exposure window has been used in the past in our laboratory (Martínez et al, 2019a(Martínez et al, , 2019bOrtiz-Villanueva et al, 2018) and it has been recognized as a good experimental window to study the effect of EDCs in zebrafish embryos by other researchers (Jarque et al, 2019). The present exposures occurred simultaneously with the ones from which metabolomic and morphometric data was obtained (Martínez et al, 2019a;Ortiz-Villanueva et al, 2018).…”

Section: Zebrafish Eleutheroembryos Exposures To Tbtmentioning

confidence: 99%

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Transcriptomic effects of tributyltin (TBT) in zebrafish eleutheroembryos. A functional benchmark dose analysis

Martínez

Codina

Barata

et al. 2020

Journal of Hazardous Materials

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Morphometric signatures of exposure to endocrine disrupting chemicals in zebrafish eleutheroembryos

Cited by 29 publications

References 127 publications

Effect of the plastic pollutant bisphenol A on the biology of aquatic organisms: A meta‐analysis

Effect of the plastic pollutant bisphenol A on the biology of aquatic organisms: A meta‐analysis

An Experimental Approach to Study the Effects of Realistic Environmental Mixture of Linuron and Propamocarb on Zebrafish Synaptogenesis

Transcriptomic effects of tributyltin (TBT) in zebrafish eleutheroembryos. A functional benchmark dose analysis

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