Morphometrical and genetic comparison of two nematode species: H. spumosa and H. dahomensis (Nematoda, Heterakidae)

Ribas, Alexis; Bellocq, Joëlle Goüy de; Ros, Albert F. H.; Ndiaye, Papa Ibnou; Miquel, Jordi

doi:10.2478/s11686-013-0156-4

Cited by 14 publications

(6 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nematode species identification referred to Ribas et al . [ 46 ], del Rosario Robles et al . [ 47 ], Durette-Desset [ 48 ], Hugot and Quentin [ 49 ], and Anderson et al .…”

Section: Methodsmentioning

confidence: 99%

Population genetics, community of parasites, and resistance to rodenticides in an urban brown rat (Rattus norvegicus) population

et al. 2017

View full text Add to dashboard Cite

Brown rats are one of the most widespread urban species worldwide. Despite the nuisances they induce and their potential role as a zoonotic reservoir, knowledge on urban rat populations remains scarce. The main purpose of this study was to characterize an urban brown rat population from Chanteraines park (Hauts-de-Seine, France), with regards to haematology, population genetics, immunogenic diversity, resistance to anticoagulant rodenticides, and community of parasites. Haematological parameters were measured. Population genetics was investigated using 13 unlinked microsatellite loci. Immunogenic diversity was assessed for Mhc-Drb. Frequency of the Y139F mutation (conferring resistance to rodenticides) and two linked microsatellites were studied, concurrently with the presence of anticoagulant residues in the liver. Combination of microscopy and molecular methods were used to investigate the occurrence of 25 parasites. Statistical approaches were used to explore multiple parasite relationships and model parasite occurrence. Eighty-six rats were caught. The first haematological data for a wild urban R. norvegicus population was reported. Genetic results suggested high genetic diversity and connectivity between Chanteraines rats and surrounding population(s). We found a high prevalence (55.8%) of the mutation Y139F and presence of rodenticide residues in 47.7% of the sampled individuals. The parasite species richness was high (16). Seven potential zoonotic pathogens were identified, together with a surprisingly high diversity of Leptospira species (4). Chanteraines rat population is not closed, allowing gene flow and making eradication programs challenging, particularly because rodenticide resistance is highly prevalent. Parasitological results showed that co-infection is more a rule than an exception. Furthermore, the presence of several potential zoonotic pathogens, of which four Leptospira species, in this urban rat population raised its role in the maintenance and spread of these pathogens. Our findings should stimulate future discussions about the development of a long-term rat-control management program in Chanteraines urban park.

show abstract

“…Nematode species identification referred to Ribas et al . [ 46 ], del Rosario Robles et al . [ 47 ], Durette-Desset [ 48 ], Hugot and Quentin [ 49 ], and Anderson et al .…”

Section: Methodsmentioning

confidence: 99%

Population genetics, community of parasites, and resistance to rodenticides in an urban brown rat (Rattus norvegicus) population

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Helminths were identified based on their morphological characteristics (Soulsby 1982;Gomes et al 2004), and a subset of samples was used for molecular confirmation and identification using DNA barcoding (Gasser and Hoste 1995;Ribas et al 2013). DNA extraction was performed using the DNeasy Blood and Tissue kit (Qiagen, USA).…”

Section: Helminth Species Identification and Dna Barcodingmentioning

confidence: 99%

Characterisation of gastrointestinal helminths and their impact in commercial small-scale chicken flocks in the Mekong Delta of Vietnam

Vân

Cương

Yen

et al. 2019

Trop Anim Health Prod

Self Cite

View full text Add to dashboard Cite

Commercial small-scale chicken farms managed as all-in-all-out but operating with low standards of hygiene/biosecurity are increasingly common in Vietnam. These conditions facilitate the transmission of gastrointestinal helminths. However, there are no published data on helminths in these systems. We aimed (1) to determine the prevalence/burden of gastrointestinal helminths in small-scale commercial flocks in the Mekong Delta region and (2) to investigate the association between worm burdens and birds' weight and disease status. Randomly selected chickens (n = 120) from 'normal' flocks were investigated at the end of their production cycle (~18 weeks), as well as 90 chickens from 'diseased' flocks with signs of respiratory and/or severe disease. The gastrointestinal tract of chickens was dissected and all visible helminths were identified and counted. A total of 54.2% and 54.4% normal and diseased chickens contained helminths. Among colonised birds, the diseased ones harboured a higher mass of helminth worms than normal (healthy) birds (3.8 ± SD 8.6 g vs. 1.9 ± SD 6.3 g, respectively). Eight species were identified, including nematodes (Ascaridia galli, Cheilospirura hamulosa and Heterakis gallinarum), cestodes (Hymenolepis, Raillietina cesticillus, Raillietina echinobothrida, Raillietina tetragona,) and one trematode (Echinostomatidae). Heterakis gallinarum was the most prevalent helminth (43.3% and 42.2% in normal and sick chickens, respectively), followed by A. galli (26.7% and 41.1%). Colonised chickens weighed 101.5 g less than non-colonised birds. Colonisation was higher during the rainy months (May-November) for both H. gallinarum and A. galli. Anthelminthic usage was not associated with reduced helminth burdens. We recommend upgrading cleaning and disinfection and limiting access to ranging areas to control helminths in small-scale chicken flocks.

show abstract

“…Helminths were identified based on their morphological characteristics (Gomes et al 2004, Soulsby 1982), and a subset of samples were used for molecular confirmation and identification using DNA barcoding (Gasser and Hoste 1995, Ribas et al 2013). DNA extraction was performed using DNeasy Blood and Tissue kit (Qiagen, USA).…”

Section: Methodsmentioning

confidence: 99%

Characterisation of gastrointestinal helminths and their impact in commercial small-scale chicken flocks in the Mekong Delta of Vietnam

Vân

Cương

Yen

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

Commercial small-scale chicken farms using all-in-all-out production but operating with low standards of hygiene/biosecurity are increasingly common in Vietnam. These conditions facilitate transmission of gastrointestinal helminths. However there are no published data on these parasites in these systems. The aims were: (1) to determine the prevalence/burden of gastrointestinal helminths in small-scale commercial flocks in commercial small-scale flocks in the Mekong Delta region; and (2) to investigate the association between worm burdens and birds’ weight and disease status. Randomly selected chickens (n=120) (‘normal’ flocks) were investigated at the end of their production cycle (∼18 weeks), as well as 90 chickens with signs of respiratory and/or severe disease. The gastrointestinal tract of chickens was dissected and all visible helminths were identified. 54.2% and 54.4% healthy and diseased chickens contained helminths. Diseased, colonized chickens harboured a higher mass of helminth worms (3.8 ±SD 8.6g) than colonized, healthy chickens (1.9 ±6.3g). Eight species were identified, three nematodes (Ascaridia galli, Cheilospirura hamulosa and Heterakis gallinarum), four cestodes (Hymenolepis, Raillietina cesticillus, Raillietina echinobothrida, Raillietina tetragona,) and one trematode (Echinostomatidae). Heterakis gallinarum was the most prevalent helminth (43.3% and 42.2% in healthy and sick chickens, respectively), followed by A. galli (26.7% and 41.1%). Colonized chickens weighed 101.5g less than non-colonized birds. Colonisation was significantly higher during the rainy months (May-November) for both H. gallinarum and A. galli. Anthelminthic usage was not associated with reduced helminth burdens. We recommend upgrading cleaning and disinfection and limiting access to ranging areas to control helminth infections in small-scale commercial chicken flocks.

show abstract

Morphometrical and genetic comparison of two nematode species: H. spumosa and H. dahomensis (Nematoda, Heterakidae)

Cited by 14 publications

References 16 publications

Population genetics, community of parasites, and resistance to rodenticides in an urban brown rat (Rattus norvegicus) population

Population genetics, community of parasites, and resistance to rodenticides in an urban brown rat (Rattus norvegicus) population

Characterisation of gastrointestinal helminths and their impact in commercial small-scale chicken flocks in the Mekong Delta of Vietnam

Characterisation of gastrointestinal helminths and their impact in commercial small-scale chicken flocks in the Mekong Delta of Vietnam

Contact Info

Product

Resources

About