Morphometry and Histochemistry of Pulmonary Arteries in a Hypoplastic Lung Model

Doolin, Edward J.; Strande, Louise; Attorri, Robert J.

doi:10.1006/jsre.1995.1153

Cited by 5 publications

(3 citation statements)

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“…Reversal of nerve‐mediated inhibition by L‐NAME and increased staining intensity in NADPH‐positive myenteric neurones in preparations from displaced abomasum suggest involvement of nitric oxide. Increased NADPH staining in myenteric neurones might be interpreted as enhanced activity of the nitric oxide synthase resulting in enhanced nitric oxide synthesis, a concept supported by other studies 25 –30 . It has been reported that nitric oxide presynaptically inhibits release of acetylcholine 31 .…”

Section: Discussionmentioning

confidence: 59%

In vitro motility disorders associated with displaced abomasum in dairy cows

Geishauser

Reiche

Schemann

1998

Neurogastroenterology Motil

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The objective of this study was to investigate in vitro abomasal motility in dairy cows diagnosed with displaced abomasum. Longitudinal muscle myenteric plexus preparations originating from the abomasal antrum of control cows, and cows diagnosed with left displaced abomasum (LDA), right displaced abomasum (RDA) or abomasal volvulus (AV) were used. In control preparations electrical field stimulation evoked an immediate cholinergic contractile response exceeding amplitude of basal contractions by 60%. In contrast, contractile activity was significantly inhibited during electrical stimulation in LDA, RDA and AV by 47%, 66% and 45%, respectively. This inhibition was reversed in the presence of L-NAME. The staining intensity of NADPH-positive myenteric neurones was significantly higher in displaced abomasa than in controls. Concentration-response curves indicated that preparations from displaced abomasa showed reduced sensitivity to acetylcholine. This study demonstrated motility disorders in displaced abomasa in vitro. The results suggested that abomasal displacement is associated with malfunctions at the level of the intrinsic nervous system combined with impaired cholinergic muscle responses. There appeared to be a predominance of nitrergic inhibitory mechanisms over excitatory mechanisms. These results might be of significance for diseases associated with gastric hypomotility and emptying disorders.

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Section: Discussionmentioning

confidence: 59%

In vitro motility disorders associated with displaced abomasum in dairy cows

Geishauser

Reiche

Schemann

1998

Neurogastroenterology Motil

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“…5.0, SPSS Science, Chicago, Ill., USA) was used to color blue the area of the image not occupied by discs (Fig. 3) (Doolin et al 1995). The masked image was saved to disk and reopened in Scion Image 3 (Ver.…”

Section: Point Fraction Of Discs (P Discs )mentioning

confidence: 99%

“…As is evident, stereological studies involving manual point and/or line intercept counts may become very laborious and time consuming, particularly when large sample sizes are used. For this reason, we explored the possibility of using computer-assisted stereology to obtain accurate values of P p (Doolin et al 1995) and S v .…”

Section: Introductionmentioning

confidence: 99%

Computer‐assisted stereology: Point fraction of lung parenchyma and alveolar surface density in fetal and newborn sheep

et al. 2003

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Summary:The aim of this study was to determine the accuracy of a new computer-assisted stereological technique in obtaining structural information of the lung. We compared the point fraction of lung parenchyma (P p ) and alveolar surface density (S v ) obtained by established manual point/intercept counting methods and compared them with those obtained using a computer-assisted method. Lung tissues obtained from normally grown fetal sheep (n=6) and from newborn lambs with severe lung hypoplasia (n=5) were inflation fixed via the trachea and processed for light microscopy. In verification-of-technique experiments, P p and S v correlated well with known values. There was a significant linear correlation between manual and computerassisted stereological measurements for values of P p (r 2 = 0.92) and S v (r 2 = 0.98). Our data lead us to believe that the computer-assisted stereological technique described in this study provides accurate estimates of P p and S v and hence may be a valuable tool for evaluating the effects of factors upon structural development of the lung.

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The Respiratory System

Gould¹

2001

Fetal and Neonatal Pathology

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Morphometry and Histochemistry of Pulmonary Arteries in a Hypoplastic Lung Model

Cited by 5 publications

References 0 publications

In vitro motility disorders associated with displaced abomasum in dairy cows

In vitro motility disorders associated with displaced abomasum in dairy cows

Computer‐assisted stereology: Point fraction of lung parenchyma and alveolar surface density in fetal and newborn sheep

The Respiratory System

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