Morphometry of Chara aspera, C. canescens, C. baltica var. baltica, C. baltica var. liljebladii and C. intermedia oospores: Local variation versus taxonomic differences

Holzhausen, Anja; Nowak, Petra; Niedrig, Claudia; Feike, Martin; Schubert, Hendrik

doi:10.1016/j.aquabot.2014.07.001

Cited by 17 publications

(8 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…), while other species are different (Holzhausen et al. ), thus supporting the results summarized in Figure .…”

Section: Discussionsupporting

confidence: 81%

“…In contrast, oospore traits were shown to be useful for species differentiation in Nitella (Sakayama et al 2004b), and this may well work also for Chara. Indeed, taxa included in cluster I cannot be differentiated by oospore traits (Blume et al 2009), while other species are different (Holzhausen et al 2015), thus supporting the results summarized in Figure 3.…”

Section: Discussionsupporting

confidence: 79%

See 1 more Smart Citation

DNA barcoding the genus Chara: molecular evidence recovers fewer taxa than the classical morphological approach

Schneider

Rodrigues

Moe

et al. 2015

Journal of Phycology

View full text Add to dashboard Cite

Charophytes (Charales) are benthic algae with a complex morphology. They are vulnerable to ecosystem changes, such as eutrophication, and are red-listed in many countries. Accurate identification of Chara species is critical for understanding their diversity and for documenting changes in species distribution. Species delineation is, however, complicated, because of high phenotypic plasticity. We used barcodes of the ITS2, matK and rbcL regions to test if the distribution of barcode haplotypes among individuals is consistent with species boundaries as they are currently understood. The study included freshly collected and herbarium material of 91 specimens from 10 European countries, Canada and Argentina. Results showed that herbarium specimens are useful as a source of material for genetic analyses for aquatic plants like Chara. rbcL and matK had highest sequence recoverability, but rbcL had a somewhat lower discriminatory power than ITS2 and matK. The tree resulting from the concatenated data matrix grouped the samples into six main groups contrary to a traditional morphological approach that consisted of 14 different taxa. A large unresolved group consisted of C. intermedia, C. hispida, C. horrida, C. baltica, C. polyacantha, C. rudis, C. aculeolata, and C. corfuensis. A second unresolved group consisted of C. virgata and C. strigosa. The taxa within each of the unresolved groups shared identical barcode sequences on the 977 positions of the concatenated data matrix. The morphological differences of taxa within both unresolved groups include the number and length of spine cells, stipulodes, and bract cells. We suggest that these morphological traits have less taxonomic relevance than hitherto assumed.

show abstract

“…), while other species are different (Holzhausen et al. ), thus supporting the results summarized in Figure .…”

Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 79%

DNA barcoding the genus Chara: molecular evidence recovers fewer taxa than the classical morphological approach

Schneider

Rodrigues

Moe

et al. 2015

Journal of Phycology

View full text Add to dashboard Cite

show abstract

“…The individual numbers of oospores examined and the appropriate pre-treatments are given in Table S2. Oospores were stored in well plates for eventual reexamination and are part of the Rostocker oospore database (Holzhausen et al, 2015).…”

Section: Oospore Analysesmentioning

confidence: 99%

Plastid DNA sequences and oospore characters of some European taxa of Tolypella section Tolypella (Characeae) identify five clusters, including one new cryptic Tolypella taxon from Sardinia, but they do not coincide with current morphological descriptions

Holzhausen

Nowak²,

Ballot³

et al. 2023

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

In Europe, the genus Tolypella (Characeae) comprises four to eight Tolypella taxa in sections Rothia and Tolypella that have been distinguished by vegetative morphology and gametangial characters such as antheridial size and oospore wall ornamentation. However, morphological differentiation is difficult in some cases due to overlapping and variable vegetative features, which in many cases are difficult to observe clearly. To clarify the taxonomic status of the five European taxa of Tolypella in section Tolypella, sequence data of the plastid genes atpB, rbcL and psbC for Tolypella glomerata (Desv.) Leonh., Tolypella hispanica Allen, Tolypella nidifica (O.F. Müll.) A. Braun, Tolypella normaniana (Nordst.) Nordst. and Tolypella salina Cor. were combined with data on oospore morphology, including oospore wall ornamentation. Gene sequence data identified five distinct clusters, but they were not consistent with the morphologically identified five taxa. T. glomerata consisted of some of the samples morphologically identified as T. glomerata and seven samples of T. normaniana, while the remaining T. glomerata samples clustered with specimens of unclear affiliation (Tolypella sp.). We identified two clusters of T. hispanica within the European material: cluster T. hispanica I consisted of samples from various locations, whereas the second cluster (T. hispanica II) consisted of samples of T. hispanica from Sardinia Island. The remaining cluster consisted of all the specimens that had been determined as T. salina or T. nidifica in addition to two specimens of T. normaniana. Oospore morphology was most clearly distinguishable for T. glomerata. Oospore characteristics for all other taxa were not as informative but showed some geographical and/or environmentally influenced differences, especially for T. nidifica and T. salina. Our results suggest the need to further check the different taxonomy of Tolypella sect. Tolypella in which specimens normally identified as T. glomerata might be two different taxa, T. glomerata and an unidentified taxon; T. nidifica and T. salina are not separate taxa; T. normaniana is a diminutive variant of two different Tolypella taxa; and T. hispanica comprises two different taxa, one from the Mediterranean island Sardinia.

show abstract

“…Species delineation of extant charophytes is commonly based on morphological traits of the thallus, while fossil charophytes usually are discriminated based on the morphology of the oospore (= zygote plus its organic walls) or the gyrogonite (formed by calcification of the spiral cells surrounding the oospore) (Holzhausen et al, 2014; this issue; Soulié-Märsche and this issue). In order to link fossil charophyte remains to extant species, it is thus important to pay more attention to oospore and gyrogonite morphology of extant charophytes, and to investigate conditions under which the female gametangium calcifies to form a gyrogonite.…”

Section: Introductionmentioning

confidence: 99%

The role of charophytes (Charales) in past and present environments: An overview

et al. 2015

View full text Add to dashboard Cite

Charophytes, i.e. extant and fossil members of the order Charales plus the members of the extinct orders Sycidiales and Moellerinales, are closely related to modern land plants. These algae have a complex morphology, and can tolerate salinities from freshwater up to hypersaline conditions, although they are not known to occur in fully marine habitats. Extant charophytes are found on all continents except Antarctica, in both lotic and lentic, natural and artificial habitats, ranging from ancient lakes to newly excavated gravel pits. The large size of the internode cells makes charophytes useful objects in plant cell biology. Charophytes can build up large biomasses in brackish and freshwater ecosystems, and contribute to a number of ecosystem services, including removal of nutrients from water, storage of carbon and nutrients in biomass and sediments, possible phytoremediation of organic chemicals and trace metal elements from water, as well as provision of habitat and food for a number of organisms. Charophytes are valuable indicators for lake and river ecological status assessment, and their oospores and gyrogonites are useful for paleolimnological 2 reconstructions of ecosystem properties such as trophic status or salinity. This paper introduces charophytes, and summarizes different aspects studied in these macroalgae. ForewordThe International Research Group on Charophytes (IRGC; http://irgc.uow.edu.au) was established in 1989, and offers a forum for scientists dedicated to the study of fossil and extant charophytes. The organization has about 120 members from around the world, and holds international conferences every fourth year. The most recent conference, the 6 th IRGC, was organized in Mendoza, Argentina in 2012, and was dedicated to honor Dr. Eduardo A.Musacchio, who began organizing the conference but died tragically in 2011. This Special Issue contains a selection of the papers presented at the 6 th IRGC conference, and covers a wide variety of topics related to morphology, ecology, paleoecology, geochemistry, ecophysiology, and ultrastructure of charophytes. We here provide an overview of the articles published in this special issue, discussed within the broader context of charophyte research.

show abstract

Morphometry of Chara aspera, C. canescens, C. baltica var. baltica, C. baltica var. liljebladii and C. intermedia oospores: Local variation versus taxonomic differences

Cited by 17 publications

References 25 publications

DNA barcoding the genus Chara: molecular evidence recovers fewer taxa than the classical morphological approach

DNA barcoding the genus Chara: molecular evidence recovers fewer taxa than the classical morphological approach

Plastid DNA sequences and oospore characters of some European taxa of Tolypella section Tolypella (Characeae) identify five clusters, including one new cryptic Tolypella taxon from Sardinia, but they do not coincide with current morphological descriptions

The role of charophytes (Charales) in past and present environments: An overview

Contact Info

Product

Resources

About