Morphometry of nuclear and nucleolar structures in a CaCo‐2 cell line

Karalyan, Zaven; Djaghatspanyan, N.G.; Gasparyan, M.H.; Hakobyan, Lilit; Abroyan, Liana; Magakyan, Y.H.; Ter-Pogossyan, Z.R.; Kamalyan, L.A.; Каралова, Е М

doi:10.1016/j.cellbi.2004.01.004

Cited by 7 publications

(6 citation statements)

References 13 publications

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“…The cDNA of one Caco-2 cell clone (clone #1) was therefore further analyzed via bacterial cloning of the ATP7B exon 2 PCR product. Sequence analysis of the bacterial clones (n = 19) suggested a compound mutation consisting of altogether three deletions (p.L394F_A395del, p.L394FfsX9 and p.E396KfsX11) corroborating the reported polyploidy of the Caco-2 cell line (S3 Table) [25]. Importantly, a wildtype sequence could not be observed in the bacterial clones.…”

Section: Generation Of a Human Atp7b Crispr/cas9 Knockout Intestinal Cell Linesupporting

confidence: 59%

ATP7B knockout disturbs copper and lipid metabolism in Caco-2 cells

et al. 2020

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Intestinal cells control delivery of lipids to the body by adsorption, storage and secretion. Copper (Cu) is an important trace element and has been shown to modulate lipid metabolism. Mutation of the liver Cu exporter ATP7B is the cause of Wilson disease and is associated with Cu accumulation in different tissues. To determine the relationship of Cu and lipid homeostasis in intestinal cells, a CRISPR/Cas9 knockout of ATP7B (KO) was introduced in Caco-2 cells. KO cells showed increased sensitivity to Cu, elevated intracellular Cu storage, and induction of genes regulating oxidative stress. Chylomicron structural protein ApoB48 was significantly downregulated in KO cells by Cu. Apolipoproteins ApoA1, ApoC3 and ApoE were constitutively induced by loss of ATP7B. Formation of small sized lipid droplets (LDs) was enhanced by Cu, whereas large sized LDs were reduced. Cu reduced triglyceride (TG) storage and secretion. Exposure of KO cells to oleic acid (OA) resulted in enhanced TG storage. The findings suggest that Cu represses intestinal TG lipogenesis, while loss of ATP7B results in OA-induced TG storage.

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Section: Generation Of a Human Atp7b Crispr/cas9 Knockout Intestinal Cell Linesupporting

confidence: 59%

ATP7B knockout disturbs copper and lipid metabolism in Caco-2 cells

et al. 2020

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“…The nucleolar DNA content is an indicator of functional condition and of cellular proliferation degree (Trére 2000; Karalyan et al. 2004).…”

Section: Discussionmentioning

confidence: 99%

“…The nucleolar DNA content is an indicator of functional condition and of cellular proliferation degree (Trére 2000;Karalyan et al 2004). In the old gerbil prostate, the number of nucleolar corpuscles increased slightly in PINs and carcinoma, while in prostatic stromal hyperplasia almost 70% of the nuclei did not present evident nucleoli.…”

Section: Discussionmentioning

confidence: 99%

Age‐related histopathological lesions in the Mongolian gerbil ventral prostate as a good model for studies of spontaneous hormone‐related disorders

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Zanetoni

Scarano

et al. 2007

Int J Experimental Path

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The Meriones unguiculatus (Mongolian) gerbil has demonstrated significant prostatic responses to hormonal treatments, and to drugs against human prostatic hyperplasia. Spontaneous neoplasia develops in the older animals. Thirty gerbils (age 18 months) were divided into non-affected and prostatic lesion bearers and the prostate lesions were evaluated morphologically, immunohistochemically and quantitatively. The most frequent changes were in epithelial sites and, namely prostatic intraepithelial neoplasias, microinvasive carcinomas and adenocarcinomas. In the stromal compartment, cellular hyperplasia, when verified, was always associated with the sites of anomalous epithelium. Additionally, larger deposition of collagen fibrils, generating stromal fibrosis, was found in all the old gerbils analysed. The quantitative analysis showed that prostatic tissue proportions differed in altered areas, being specific for each lesion type. Isolated nuclear and nucleolar parameters were not effective in diagnosing the malign potential of lesions. However, the cellular proliferation and death indexes indicated larger cellular turnover in invasive lesions such as carcinomas. With these analyses, it could be verified that old gerbils present high propensity to develop spontaneous prostate changes and this may aid in a better understanding of the biological behaviour of human prostate cancer.

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“…The nucleolus/nucleus ratio showed that dimensional variations undergone by the nuclei are accompanied by the nucleoli, which have a functional complementation among these organelles. Nucleolar DNA content is an indicator of functional condition and the degree of cellular proliferation (Karalyan et al,2004). Studies have shown that quantitative analysis of AgNOR proteins provides additional information concerning the biological behavior of tumors, being an independent prognostic factor (Ceccarelli et al,2000; Öfner,2000).…”

Section: Discussionmentioning

confidence: 99%

Biological behavior of the gerbil ventral prostate in three phases of postnatal development

et al. 2006

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In this study, we characterized the gerbil's ventral prostate histology ultrastructurally and quantitatively throughout three phases of postnatal development (young, adult, and old) in order to comprehend its biological behavior and propensity to developing spontaneous lesions with aging. The gerbil prostate is composed of alveoli and ducts immersed in a stroma composed of smooth muscle, fibroblasts, collagen and elastic fibers and vessels. The prostate tissue components present morphological and quantitative aspects that vary according to age. Young animals have an immature gland with modest secretory activity. Synthetic activity remained stable in adult and old gerbil. However, prostatic morphology was altered in the aging, showing an increased epithelium and stromal fibrosis. The nuclei of the secretory cells increased with aging, whereas nucleoli presented few alterations during postnatal development. The epithelial proliferation and stromal remodeling noted in this study indicate that the gerbil prostate may respond to the androgen declines typical of senescence through epithelial proliferation and stromal remodeling. Anat Rec Part A, 288A: 723-733, 2006.

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Morphometry of nuclear and nucleolar structures in a CaCo‐2 cell line

Cited by 7 publications

References 13 publications

ATP7B knockout disturbs copper and lipid metabolism in Caco-2 cells

ATP7B knockout disturbs copper and lipid metabolism in Caco-2 cells

Age‐related histopathological lesions in the Mongolian gerbil ventral prostate as a good model for studies of spontaneous hormone‐related disorders

Biological behavior of the gerbil ventral prostate in three phases of postnatal development

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