Morphometry of porcine spermatozoa and its functional significance in relation with the motility parameters in fresh semen

Gil, María Concepción Robles; García-Herreros, Manuel; Barón, Francisco; Aparicio, I.M.; Santos, Anadalvo Juazeiro dos; García‐Marín, Luis J.

doi:10.1016/j.theriogenology.2008.07.007

Cited by 84 publications

(72 citation statements)

References 37 publications

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“…A sperm tail (flagellum) is an important organelle which determines sperm velocity and potential ability of a spermatozoon to successfully fertilize an ovum. There are some works which demonstrate an association between sperm tail length, mid-piece in particular, and sperm motility (Katz and Drobnis 1990, Gil et al 2009, Lü pold et al 2009). According to Gomendio and Roldan (1991), sperm length is positively correlated with sperm velocity.…”

Section: Discussionmentioning

confidence: 99%

Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars

Kondracki¹,

Wysokińska²,

Iwanina³

et al. 2011

Polish Journal of Veterinary Sciences

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The experimental material consisted of 75 ejaculates collected form 8 Duroc boars. The ejaculates were divided into three groups according to sperm concentration in an ejaculate. An ejaculate was obtained from each boar monthly and it was used to make microscopic preparations to examine spermatozoa morphology. In each preparation morphometric measurements were taken of fifteen randomly selected spermatozoa characterized by normal morphology. The following measurements of spermatozoa were taken: length and width of the spermatozoa head, head area, length of the flagellum, perimeter of the spermatozoon head and total spermatozoon length. The results were used to calculate indicators of spermatozoa morphology. Moreover, assessments were made of frequency of morphological defects to isolate spermatozoa with primary and secondary abnormalities following the Blom classification system. It was found that the concentration of spermatozoa in the ejaculate influenced the morphometric characteristics of spermatozoa. Ejaculates with low sperm concentrations are characterized by larger spermatozoa as compared to ejaculates with high sperm concentrations. However, sperm concentration in the ejaculate does not much influence the shape of spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars

Kondracki¹,

Wysokińska²,

Iwanina³

et al. 2011

Polish Journal of Veterinary Sciences

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“…The spermatozoa head, the mid-piece, and the tail develop concurrently (Gil et al, 2009); therefore, it has been hypothesized that the presence of cytoplasmic droplets is associated with abnormal head measurements and chromatin instability. Spermatozoa head morphometry has shown that some of these measurements correlate with female reproductive performance (Hirai et al, 2001), with chromatin destabilization (Hingst et al, 1995;Karabinus et al, 1997), and with the presence of abnormalities in the ejaculate (Gaggini et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Head morphometry and chromatin instability in normal boar spermatozoa and in spermatozoa with cytoplasmic droplets

Gaggini¹,

Rocha²,

Souza³

et al. 2017

Anim Reprod

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In boar studs, morphological analyses are used to evaluate sperm quality and thereby categorize ejaculates as either approved or rejected. Normally, morphological characteristics correlate with chromatin disorders, but studies to date have only considered the average of abnormalities; cells were not segregated as normal or abnormal. The aim of this study was to assess whether the presence of cytoplasmic droplets was associated with morphometric characteristics and chromatin instability of spermatozoa heads. Morphological analyses were performed on semen from 11 boars using phase contrast microscopy (200 cells per sample). Normal cells were differentiated from those with cytoplasmic droplets and both types were evaluated separately. Photomicrographs were acquired of normal spermatozoa (Group NOR, N = 1,207) as well as spermatozoa with proximal and distal cytoplasmic droplets (Group DROP, N = 725). Sperm-head morphometry and chromatin structure were evaluated using the toluidine blue technique. Spermatozoa heads in the DROP group were longer (8.37 ± 0.60 × 8.31 ± 0.53; P = 0.025), narrower (4.16 ± 0.21 × 4.19 ± 0.19; P = 0.03), and more symmetric on the sides (0.973 ± 0.012 × 0.971 ± 0.011; P = 0.007) than were spermatozoa heads of the NOR group. The DROP group also had a greater average ellipticity (0.335 ± 0.034 × 0.329 ± 0.031; P = 0.0004), a greater percentage of decondensed chromatin (2.71 ± 3.87 × 2.28 ± 1.38; P < 0.0008), and a greater chromatin heterogeneity (4.66 ± 1.40 × 4.40 ± 1.42; P < 0.0001). A greater frequency of semen collection results in a shorter period of cell maturation and this probably affected the degree of chromatin condensation and the cytoplasmic droplet migration, with concomitant effect on the head morphometry measurements. In conclusion, compared with normal spermatozoa, those with cytoplasmic droplets show altered morphometric characteristics, such as longer and narrower spermatozoa heads. They likewise have greater chromatin instability, resulting in a higher percentage of decondensed chromatin and more heterogeneous chromatin.

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“…A high rate of morphologic abnormality in the ejaculate has been correlated with lowered fertility [2][3][4][5][6]. The morphology and motility may be related to sperm survival and ability to fertilize the ovum [7,8]. We hypothesized that the morphology and motility of spermatozoa in semen analysis are interrelated with each other and with total sperm count in the ejaculate.…”

Section: Introductionmentioning

confidence: 99%

Alterations in the morphology and motility of spermatozoa: relation with total sperm count

Karabulut¹,

Tekin²

2013

Pau Med J

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Purpose: The aim of this study was to investigate the differences in sperm morphology and motility in patients with different sperm counts, and to search the influence of sperm morphology over sperm motility. Materials and methods:We evaluated semen analysis of 1174 males admitted to infertility clinic using "Makler counting camber" for evaluation of number and motility, and strict criteria (Kruger-Tyberg) for the morphological assessment. According to total sperm count, patients were divided into three groups; group I (n=119) had <5x10 6 /mL, group II (n=125) had 5-15x10 6 /mL, and group III (n=930) had ≥15x10 6 /mL total sperm count. The groups were compared in terms of motility, morphology and the distribution of sperm abnormalities. Results: Sperm motility was significantly lower in groups I-II compared to group III (p<0.01). In the semen analysis, normal sperm morphology above 4% was detected in 7.1% of group II, 17.5% of group III, and none in group I (p<0.01). Significantly less tail defects were detected in group III compared to others (p<0.01). Progressive sperm motility significantly correlated with morphology (r=0.38, p<0.001). Among morphologic abnormalities, tail problems were highly correlated with nonmotile sperm count (r=0.30, p<0.001). Tail defects correlated positively with midpiece (r=0.24, p<0.001), and negatively with head defects (r=-0.71, p<0.001). Normal sperm morphology had a negative relation with tail and midpiece defects (r=-0.20, p <0.001 and r=-0.30, p<0.001 respectively). Conclusion: Sperm motility and morphology were deteriorated with the decreasing sperm count in subfertile population. The chance of finding normal morphology above the critical value of 4% diminishes in cases with sperm counts less than 5x10 6 /mL. Therefore, it is logical to canalize these patients directly to assisted reproductive technologies.Pam Med J 2013;6(1):1-4

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Morphometry of porcine spermatozoa and its functional significance in relation with the motility parameters in fresh semen

Cited by 84 publications

References 37 publications

Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars

Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars

Head morphometry and chromatin instability in normal boar spermatozoa and in spermatozoa with cytoplasmic droplets

Alterations in the morphology and motility of spermatozoa: relation with total sperm count

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