Background: Low- and middle-income countries experience higher burn-related morbidity and mortality compared to high-income countries. Prehospital Burn First Aid (BFA) has been proven effective in reducing such outcomes. This study aimed to assess BFA knowledge and water lavage practices and their associated factors among burn victims, their caretakers and visitors at a tertiary health facility in Uganda. Methods: A cross-sectional study was conducted at the Burns Unit of Kiruddu National Referral Hospital in Kampala between April and November 2022. Participants included burn patients, caregivers, and visitors. Data on BFA knowledge and practices were collected using an interviewer-administered questionnaire developed from a literature review. BFA knowledge was evaluated using 13 questions, with ≥80% score considered adequate. Water lavage usage was assessed following burn injuries. Logistic regression was used to analyze associations.Ehical approval was obtained at Mulago Hospital IRB (ID: MHREC2226). Results: The study had 404 participants, comprising 68(16.8%) burn victims, 161(39.9%) primary caregivers, and 175(43.3%) visitors. Among all participants, 339(83.9%) had never received BFA information, and 392(97.0%) had no first aid training. Mean BFA knowledge score was 56% (SD 13.9), with only 5.4% demonstrating adequate knowledge. Only 26(27.7%) of current/former burn victims used water lavage as BFA. No statistically significant associations were found between BFA knowledge, water lavage usage, and demographic variables on univariate and multivariate analyses. Conclusion: This study highlights inadequate BFA knowledge and practices among participants. Addressing these deficiencies through community-based initiatives in the Ugandan population is crucial to improving burn care in Uganda.