1979
DOI: 10.1016/0022-2836(79)90545-x
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Mosaic adenovirus-SV40 RNA specified by the non-defective hybrid virus Ad2 + ND4

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Cited by 19 publications
(29 citation statements)
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“…In contrast, the progenitor virus Ad2+ND4, from which Ad2+ND4dej has been repeatedly isolated after a single infectious cycle in human cells, readily expresses its SV40 sequences in monkey cells and replicates in these cells. These two viruses differ only in that Ad2+ND4del has a deletion entirely within the SV40 sequences which removes the normal SV40 early region splice sites from Ad2+ND4 (19,38). Since the y leader is present on both genomes yet only Ad2+ND4 can efficiently synthesize the SV40-specific polypeptides in monkey cells, the presence of this sequence on chimeric Ad2-SV40 mRNAs may not be necessary for efficient utilization.…”
Section: Discussionmentioning
confidence: 99%
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“…In contrast, the progenitor virus Ad2+ND4, from which Ad2+ND4dej has been repeatedly isolated after a single infectious cycle in human cells, readily expresses its SV40 sequences in monkey cells and replicates in these cells. These two viruses differ only in that Ad2+ND4del has a deletion entirely within the SV40 sequences which removes the normal SV40 early region splice sites from Ad2+ND4 (19,38). Since the y leader is present on both genomes yet only Ad2+ND4 can efficiently synthesize the SV40-specific polypeptides in monkey cells, the presence of this sequence on chimeric Ad2-SV40 mRNAs may not be necessary for efficient utilization.…”
Section: Discussionmentioning
confidence: 99%
“…Alternatively, the diminished amounts of SV40-specific polypeptides in Ad2+ND4del-infected monkey cells compared with Ad2+ND4-infected monkey cells may result from depressed mRNA levels. The deletion in Ad2+ND4del is thought to alleviate the block to expression of the fiber gene in Ad2+ND4 infections (19,32,38) and may do so by concomitant depression of the upstream SV40-specific mRNAs and elevation of the downstream fiber mRNAs. A third possibility is that some other sequence present in Ad2+ND4 but deleted from Ad2+ND4del substitutes for the y leader in the functional, chimeric Ad2-SV40 mRNAs of Ad2+ND4.…”
Section: Discussionmentioning
confidence: 99%
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“…To define more precisely the DNA-binding properties of the 56 000 and the 42 000 M r proteins, we repeated this type of experiment under identical conditions using radiolabeled extracts of HeLa cells infected with Ad2*ND2 which codes for only two T-Ag related fragments, the 56 000 M r (0.44-0.17 SV40 DNA map units, MU) and 42 000 M r (0.39-0.17 SV40 DNA MU) proteins [14]. At all pH-values the 42 000 M r protein remained unbound in the DNAcellulose supernatant while the 56 000 M r protein showed again some binding activity at pH 6.0 (fig.…”
Section: Dna-binding Of S V40-specific Pro Reins Encoded By the Hybrimentioning
confidence: 99%
“…Ad2*ND2 and Ad2*ND4 [14] the right-end of a domain necessary for the non-specific DNA-binding properties of T-Ag can be mapped between 0.42-0.39 SV40 DNA map units. Additionally, our results agree with observations which allow one to restrict the leftend of the domain at --,0.53 MU: an 84 000 M r protein, which has the N-terminal part in common with full length T-Ag, but misses a C-terminal region between 0.25-0.17 MU, shows a DNA-binding affinity comparable to full length T-Ag [ 17]; the 33 000 M r protein encoded by the SV40 deletion mutant dl t001 (0.64-0.43 MU) binds to DNA with a high affinity [20]; SV40 small t-antigen (0.64-0.54 SV40 MU) in our hands and in other laboratories [8,16] had no DNA-binding affinity; an 82 000 M r in vitro translation product of early SV40 mRNA (0.53-0.17 MU) has a normal DNA-binding behavior [8].…”
Section: Binding Of Ad2*nd2 Hybrid Virus Encoded Proteins To Dna In Tmentioning
confidence: 99%