Mosquito and mammalian cells grown on microcarriers for four‐serotype dengue virus production: Variations in virus titer, plaque morphology, and replication rate

Liu, Chia-Chyi; Wu, Suh-Chin

doi:10.1002/bit.10918

Cited by 11 publications

(8 citation statements)

References 28 publications

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“…Chromatography (Attebery, 1975;Rocca and Rouchouse, 1976;Fahlvik et al, 1990;Zhang and El Rassi, 1999;Spegel et al, 2001) Imaging (Cuthbertson et al, 2003;Cavalieri et al, 2005;Huang et al, 2006;Klibanov, 2006) Filling of defects (Schepers et al, 1991;Guicheux et al, 1997;Santos et al, 1998;Schepers et al, 1998;Falaize et al, 1999;Huygh et al, 2002;Day et al, 2004;Domingues et al, 2004;Gosain, 2004) Adjuvants in vaccines (Ohagan et al, 1993;Moore et al, 1995;Nakaoka et al, 1995;Ertl et al, 1996;Heritage et al, 1996;Ohagan et al, 1997;Stertman et al, 2006) Cell culture (Malda et al, 2003b;Xu et al, 2003;Zhang et al, 2003;Liu and Wu, 2004;Yokomizo et al, 2004;Hong et al, 2005;Melero-Martin et al, 2006) Drug delivery (Herrmann and Bodmeier, 1995;Guicheux et al, 1997;Berthold et al, 1998;Herrmann and Bodmeier, 1998;Jeong et al, 1998;Cruaud et al, 1999;Ganza-Gonzalez et al, 1999;Lam et al, 2000;Lim et al, 2000;…”

Section: Applications In the Biomedical Field Referencesmentioning

confidence: 99%

Materials in particulate form for tissue engineering. 1. Basic concepts

Silva

Ducheyne

Reis

2007

J Tissue Eng Regen Med

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For biomedical applications, materials small in size are growing in importance. In an era where 'nano' is the new trend, micro-and nano-materials are in the forefront of developments. Materials in the particulate form aim to designate systems with a reduced size, such as micro-and nanoparticles. These systems can be produced starting from a diversity of materials, of which polymers are the most used. Similarly, a multitude of methods are to produce particulate systems, and both materials and methods are critically reviewed here. Among the varied applications that materials in the particulate form can have, drug delivery systems are probably the most prominent, as these have been in the forefront of interest for biomedical applications. The basic concepts pertaining to drug delivery are summarized, and the role of polymers as drug delivery systems conclude this review. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE R E V I E W A R T I C L E

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Section: Applications In the Biomedical Field Referencesmentioning

confidence: 99%

Materials in particulate form for tissue engineering. 1. Basic concepts

Silva

Ducheyne

Reis

2007

J Tissue Eng Regen Med

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“…The development of microcarriers for increasing cell density and ultimately product yield have been advantageous in biotechnology, especially for adherent cell types (1). Solid microcarriers such as Cytodex have been used extensively for the growth of anchorage-dependent cells, particularly in virus production (2)(3)(4)(5) and for recombinant protein production (6,7) and increasingly for the growth of primary cells (8)(9)(10). Coating with collagen, gelatin, or laminin is often used to improve the attachment of some cell types.…”

Section: Introductionmentioning

confidence: 99%

Production and Glycosylation of Recombinant β-Interferon in Suspension and Cytopore Microcarrier Cultures of CHO Cells

Spearman

Rodrı́guez

Huzel

et al. 2008

Biotechnol Progress

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Microcarriers are suitable for high-density cultures of cells requiring surface attachment and also offer the advantage of easy media removal for product recovery. We have used the macroporous microcarriers Cytopore 1 and 2 for the growth of CHO cells producing recombinant human beta-interferon (beta-IFN) in stirred batch cultures. Although these cells may grow in suspension, in the presence of Cytopore microcarriers they become entrapped in the inner bead matrix where they can be maintained at high densities. Cell growth rates were reduced in microcarrier cultures compared to suspension cultures. However, the beta-IFN yield was up to 3-fold greater as a result of an almost 5-fold higher specific productivity. Maximum productivity was found in cultures containing 1.0 mg/mL of Cytopore 1 or 0.5 mg/mL of Cytopore 2 with a cell/bead ratio of 1029 and 822, respectively. Beta-IFN molecules aggregated in the later stages of all cultures, causing a decrease in response by ELISA. However, the degree of aggregation was significantly less in the microcarrier cultures. The N-linked glycans from beta-IFN were isolated and analyzed by normal phase HPLC. There was no apparent difference in the profile of glycans obtained from each of the suspension and Cytopore culture systems. This suggests that Cytopore microcarriers may be useful in bioprocess development for enhanced recombinant glycoprotein production without affecting the glycosylation profile of the protein.

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“…CR2 cells cultured on Cytodex 1 was used for the purification of DISC‐HSV virus 5. The mosquito and mammalian cells cultured on Cytodex 1 were used for four‐serotype dengue virus production 6. Cytodex 3 was properly used for cultivation of hepatocytes, hepatocarcinoma cells, Vero cells, Fe cells for the production of canine parvovirosis, and NM57 cell line for the production of respiratory syncytial virus (RSV) 7–10.…”

Section: Introductionmentioning

confidence: 99%

Polyethylene glycol‐based cationically charged hydrogel beads as a new microcarrier for cell culture

et al. 2006

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A new polyethylene glycol (PEG) based microcarrier was designed and examined by the attachment and growth of mouse fibroblast cells. In the design of microcarrier, a PEG-based macromonomer, polyethyleneglycol methacrylate (PEGMA), was selected as the main component of hydrogel beads since PEG is known as a nontoxic and biocompatible material. A relatively new cationic comonomer, N-[3-(dimethylamino)propyl]methacrylamide (DMAPM), with higher ionization ability with respect to the similar comonomers was used for providing cationic charge to the hydrogel structure. In the first part, a suspension copolymerization method was developed for the production of cationically charged hydrogel beads as a potential microcarrier for cell culturing. The suspension copolymerization by using ethylene dimethacrylate (EDM) as cross-linking agent and cyclohexanol as the diluent provided spherical, polydisperse poly(PEGMA-DMAPM-EDM) hydrogel beads with an average size of 121 microm. The hydrogel beads exhibited a pH-dependent swelling behavior. The L929 mouse fibroblast cells were cultured on poly(PEGMA-DMAPM-EDM) hydrogel beads with an initial concentration of 200,000 cells/mL. The cells were incubated in Dulbecco's modified Eagle's medium during 5 days and the cell proliferation was investigated at every 24 h. An effective cell attachment and growth up to 3.5 x 10(6) cells/mL were observed with the poly(PEGMA-DMAPM-EDM) hydrogel beads. The results indicated that the proposed microcarrier was a significant alternative to the hydrogel beads obtained by the copolymerization of 2-hydroxyethyl methacrylate and 2-dimethylaminoethylmethacrylate commonly used in microcarrier-facilitated cell culturing studies.

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Mosquito and mammalian cells grown on microcarriers for four‐serotype dengue virus production: Variations in virus titer, plaque morphology, and replication rate

Cited by 11 publications

References 28 publications

Materials in particulate form for tissue engineering. 1. Basic concepts

Materials in particulate form for tissue engineering. 1. Basic concepts

Production and Glycosylation of Recombinant β-Interferon in Suspension and Cytopore Microcarrier Cultures of CHO Cells

Polyethylene glycol‐based cationically charged hydrogel beads as a new microcarrier for cell culture

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