Mosquito<i>Aedes aegypti</i>(L.) leucokinin receptor is critical for<i>in vivo</i>fluid excretion post blood feeding

Kersch, Cymon; Pietrantonio, Patricia V.

doi:10.1016/j.febslet.2011.10.001

Cited by 39 publications

(28 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found that AKIII did not detectably modulate contractions of the crop. Consistent with this negative data, mRNAs encoding the kinin receptor are weakly expressed in the crop of D. melanogaster , and kinin or kinin-receptor immunoreactivities have not been reported in the crop of D. melanogaster or A. aegypti (Al-Anzi et al 2010; Chintapalli et al 2007; Kersch and Pietrantonio, 2011). …”

Section: Discussionsupporting

confidence: 76%

Physiological characterization and regulation of the contractile properties of the mosquito ventral diverticulum (crop)

Calkins

DeLaat

Piermarini

2017

Journal of Insect Physiology

View full text Add to dashboard Cite

In adult dipteran insects (flies), the crop is a diverticulum of the esophagus that serves as a food storage organ. The crop pumps stored contents into the alimentary canal for digestion and absorption. The pumping is mediated by peristaltic contractions of the crop musculature. In adult female mosquitoes, the crop (ventral diverticulum) selectively stores sugar solutions (e.g., nectar); proteinaceous blood meals by-pass the crop and are transferred directly to the midgut for digestion. The mechanisms that regulate crop contractions have never been investigated in mosquitoes. Here we provide the first physiological characterization of the contractile properties of the mosquito crop and explore the mechanisms that regulate crop contractions. Using an in vitro bioassay we found that the isolated crop spontaneously contracts in Ringer solution for at least 1 hour and its contractions are dependent on extracellular Ca2+. Adding serotonin (5- hydroxytryptamine, 5-HT) or a membrane-permeable analog of cyclic adenosine monophosphate (cAMP) to the extracellular bath increased the frequency of crop contractions. On the other hand, adding benzethonium chloride (BzCl; a chemical that mimics the effects of myosuppressins), H-89 or Rp-cAMPS (inhibitors of protein kinase A, PKA), or carbenoxolone (an inhibitor of gap junctions) reduced the frequency of the unstimulated, spontaneous and/or 5-HT-stimulated crop contractions. Adding aedeskinin III did not detectably alter crop contraction rates. In addition to pharmacological evidence of gap junctions, we demonstrated that the crop expressed several mRNAs encoding gap junctional proteins (i.e. innexins). Furthermore, we localized immunoreactivity for innexin 2 and innexin 3 to muscle and epithelial cells of the crop, respectively. Our results 1) suggest that 5-HT and myosupressins oppositely regulate contractile activity of the mosquito crop, and 2) provide the first evidence for putative roles of cAMP, PKA, and gap junctions in modulating contractile activity of the dipteran crop.

show abstract

Section: Discussionsupporting

confidence: 76%

Physiological characterization and regulation of the contractile properties of the mosquito ventral diverticulum (crop)

Calkins

DeLaat

Piermarini

2017

Journal of Insect Physiology

View full text Add to dashboard Cite

show abstract

“…These tissues also displayed elevated Ca 2+ in response to myokinin, demonstrating novel roles for these neuropeptides (Radford et al, 2002). In A. aegypti the myokinin receptor was shown to be critical for in vivo fluid excretion post blood feeding (Kersch and Pietrantonio, 2011). In Drosophila the receptor was shown to be involved in appetite, chemosensory responses, and metabolism (Al-Anzi et al, 2010; de Haro et al, 2010; Cognigni et al, 2011; López-Arias et al, 2011).…”

Section: Deorphanized Neuropeptide Receptorsmentioning

confidence: 99%

More than two decades of research on insect neuropeptide GPCRs: an overview

Caers¹,

Verlinden²,

Zels³

et al. 2012

Front. Endocrin.

154

143

View full text Add to dashboard Cite

This review focuses on the state of the art on neuropeptide receptors in insects. Most of these receptors are G protein-coupled receptors (GPCRs) and are involved in the regulation of virtually all physiological processes during an insect's life. More than 20 years ago a milestone in invertebrate endocrinology was achieved with the characterization of the first insect neuropeptide receptor, i.e., the Drosophila tachykinin-like receptor. However, it took until the release of the Drosophila genome in 2000 that research on neuropeptide receptors boosted. In the last decade a plethora of genomic information of other insect species also became available, leading to a better insight in the functions and evolution of the neuropeptide signaling systems and their intracellular pathways. It became clear that some of these systems are conserved among all insect species, indicating that they fulfill crucial roles in their physiological processes. Meanwhile, other signaling systems seem to be lost in several insect orders or species, suggesting that their actions were superfluous in those insects, or that other neuropeptides have taken over their functions. It is striking that the deorphanization of neuropeptide GPCRs gets much attention, but the subsequent unraveling of the intracellular pathways they elicit, or their physiological functions are often hardly examined. Especially in insects besides Drosophila this information is scarce if not absent. And although great progress made in characterizing neuropeptide signaling systems, even in Drosophila several predicted neuropeptide receptors remain orphan, awaiting for their endogenous ligand to be determined. The present review gives a précis of the insect neuropeptide receptor research of the last two decades. But it has to be emphasized that the work done so far is only the tip of the iceberg and our comprehensive understanding of these important signaling systems will still increase substantially in the coming years.

show abstract

“…1), especially in relation to signalling cascades initiated by neuropeptides . Mosquito tubules also contain principal and stellate cells, and research into cell signalling mechanisms in mosquito tubules has revealed important insights into control of epithelial function in blood-feeding insects via both cell types (Coast et al, 2005;Kersch and Pietrantonio, 2011;Pollock et al, 2004;Radford et al, 2004;Schepel et al, 2010).…”

Section: The Malpighian Tubules As Stress Sensorsmentioning

confidence: 99%

Cell signalling mechanisms for insect stress tolerance

Davies

Cabrero

Overend

et al. 2014

Journal of Experimental Biology

View full text Add to dashboard Cite

Insects successfully occupy most environmental niches and this success depends on surviving a broad range of environmental stressors including temperature, desiccation, xenobiotic, osmotic and infection stress. Epithelial tissues play key roles as barriers between the external and internal environments and therefore maintain homeostasis and organismal tolerance to multiple stressors. As such, the crucial role of epithelia in organismal stress tolerance cannot be underestimated. At a molecular level, multiple cell-specific signalling pathways including cyclic cAMP, cyclic cGMP and calcium modulate tissue, and hence, organismal responses to stress. Thus, epithelial cell-specific signal transduction can be usefully studied to determine the molecular mechanisms of organismal stress tolerance in vivo. This review will explore cell signalling modulation of stress tolerance in insects by focusing on cell signalling in a fluid transporting epithelium -the Malpighian tubule. Manipulation of specific genes and signalling pathways in only defined tubule cell types can influence the survival outcome in response to multiple environmental stressors including desiccation, immune, salt (ionic) and oxidative stress, suggesting that studies in the genetic model Drosophila melanogaster may reveal novel pathways required for stress tolerance.

show abstract

MosquitoAedes aegypti(L.) leucokinin receptor is critical forin vivofluid excretion post blood feeding

Cited by 39 publications

References 27 publications

Physiological characterization and regulation of the contractile properties of the mosquito ventral diverticulum (crop)

Physiological characterization and regulation of the contractile properties of the mosquito ventral diverticulum (crop)

More than two decades of research on insect neuropeptide GPCRs: an overview

Cell signalling mechanisms for insect stress tolerance

Contact Info

Product

Resources

About