Motile chondrocytes from newborn calf: migration properties and synthesis of collagen II

Chang, Chi Young; Lauffenburger, Douglas A.; Morales, Teresa I.

doi:10.1016/s1063-4584(03)00087-6

Cited by 72 publications

(74 citation statements)

References 43 publications

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“…Our results indicate that BSA inhibits chemotaxis of MSCs in the TCA, whether it is used in the cell buffer or in the chemoattractant buffer. We also found that premigration attachment time, employed in some studies with mesenchymal cell types (57,58), significantly reduced cell migration. This may not be surprising because it is well established that optimal migration depends on a fine balance between attachment and detachment from the substrate (25).…”

Section: Transmembrane Chemotactic Assay Optimized By Removal Of Bsa supporting

confidence: 65%

“…We initially examined the effect of attachment time used to allow MSCs to settle and adhere to the membrane before establishing a gradient of soluble chemoattractant (57,58). When no premigration cell attachment time was allowed before adding the chemoattractant in the bottom chamber, 20% of all rabbit bone marrow-derived MSCs migrated toward 10 ng/ml PDGF-BB during 4 h (Fig.…”

Section: Optimization Of the Transmembrane Chemotaxis Assaymentioning

confidence: 99%

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Fibronectin, Vitronectin, and Collagen I Induce Chemotaxis and Haptotaxis of Human and Rabbit Mesenchymal Stem Cells in a Standardized Transmembrane Assay

Thibault

Hoemann

Buschmann

2007

Stem Cells and Development

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The mesenchymal stem cell (MSC) is a critical element in tissue repair and regeneration. Its ability to differentiate into multiple connective tissue cell types and to self-renew has made it a prime candidate in regenerative medicine strategies. Currently, the environmental cues responsible for in situ recruitment and control of MSC distribution at repair sites are not entirely revealed and in particular the role of extracellular matrix (ECM) proteins as motogenic factors has not been studied. Here we have used a standardized transmembrane chemotaxis assay to assess the chemotactic and haptotactic potential of fibronectin, vitronectin, and collagen type 1 on MSCs from both rabbit and human origin. The use of both cell types was based in part on the widespread use of rabbit models for musculoskeletal-related tissue engineering and repair models and their unknown correspondence to human in terms of MSC migration. The optimized assay yielded a greatly increased chemotactic response toward known factors such as platelet-derived growth factor-BB (PDGF)-BB compared to previous studies. Our primary finding was that all three ECM proteins tested (fibronectin, vitronectin, and collagen I) induced significant motogenic activity, in both soluble and insoluble forms, for both rabbit and human MSCs. These results suggest that ECM proteins could play roles as significant as cytokines in the recruitment of pluripotential repair cells wound and tissue repair sites. Furthermore, designed ECM coatings of scaffolds or implants could provide a new tool to control both cell influx and outflux from the scaffold post-implantation. Finally, the similarity of motogenic behavior of both rabbit and human cells suggests the rabbit is a reliable model for assessing MSC recruitment in repair and regeneration strategies. 489

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Section: Transmembrane Chemotactic Assay Optimized By Removal Of Bsa supporting

confidence: 65%

Section: Optimization Of the Transmembrane Chemotaxis Assaymentioning

confidence: 99%

Fibronectin, Vitronectin, and Collagen I Induce Chemotaxis and Haptotaxis of Human and Rabbit Mesenchymal Stem Cells in a Standardized Transmembrane Assay

Thibault

Hoemann

Buschmann

2007

Stem Cells and Development

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“…A distinct advantage of the ACI procedure is the utilization of chondrocytes that produce appropriate ECM to replace the cartilage defect. Because chondrocytes have limited motility in vitro, [39][40][41] it is reasonable to assume that the native chondrocytes are unlikely to migrate a significant distance into the implanted biomaterial scaffold. We did not find such cells in the non-cell seeded scaffolds of the control group.…”

Section: Discussionmentioning

confidence: 99%

Repair of porcine articular cartilage defect with a biphasic osteochondral composite

Jiang

Chiang

Liao³

et al. 2007

Journal Orthopaedic Research

136

105

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Autologous chondrocyte implantation (ACI) has been recently used to treat cartilage defects. Partly because of the success of mosaicplasty, a procedure that involves the implantation of native osteochondral plugs, it is of potential significance to consider the application of ACI in the form of biphasic osteochondral composites. To test the clinical applicability of such composite construct, we repaired osteochondral defect with ACI at low cell-seeding density on a biphasic scaffold, and combined graft harvest and implantation in a single surgery. We fabricated a biphasic cylindrical porous plug of DL-poly-lactide-co-glycolide, with its lower body impregnated with b-tricalcium phosphate as the osseous phase. Osteochondral defects were surgically created at the weight-bearing surface of femoral condyles of Lee-Sung mini-pigs. Autologous chondrocytes isolated from the cartilage were seeded into the upper, chondral phase of the plug, which was inserted by press-fitting to fill the defect. Defects treated with cell-free plugs served as control. Outcome of repair was examined 6 months after surgery. In the osseous phase, the biomaterial retained in the center and cancellous bone formed in the periphery, integrating well with native subchondral bone with extensive remodeling, as depicted on X-ray roentgenography by higher radiolucency. In the chondral phase, collagen type II immunohistochemistry and Safranin O histological staining showed hyaline cartilage regeneration in the experimental group, whereas only fibrous tissue formed in the control group. On the International Cartilage Repair Society Scale, the experimental group had higher mean scores in surface, matrix, cell distribution, and cell viability than control, but was comparable with the control group in subchondral bone and mineralization. Tensile stress-relaxation behavior determined by uni-axial indentation test revealed similar creep property between the surface of the experimental specimen and native cartilage, but not the control specimen. Implanted autologous chondrocytes could survive and could yield hyaline-like cartilage in vivo in the biphasic biomaterial construct. Pre-seeding of osteogenic cells did not appear to be necessary to regenerate subchondral bone. ß

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“…Furthermore, the chemoattractive effects of these growth factors may enhance integration with surrounding tissue by further stimulating chondrocytes to migrate toward the scaffolds. [218][219][220] Hydrogels have long been popular as controlled delivery systems for proteins, because release profiles can be modulated by changing the hydrogel crosslinking density, which changes the free space available for diffusion. 228 The mechanisms of release can also be varied by manipulating interactions between encapsulated proteins and the hydrogel polymer, such as charge interactions, or by changing the degradation profiles of the hydrogels.…”

Section: Controlled Release Of Growth Factorsmentioning

confidence: 99%

Hydrogels for the Repair of Articular Cartilage Defects

Spiller

Maher

Lowman

2011

Tissue Engineering Part B: Reviews

404

312

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Motile chondrocytes from newborn calf: migration properties and synthesis of collagen II

Abstract: A population of well-differentiated chondrocytes capable of matrix (COL II) synthesis are motile in vitro. This original finding opens new avenues to study the potential of motile cells for cartilage repair.

Cited by 72 publications

References 43 publications

Fibronectin, Vitronectin, and Collagen I Induce Chemotaxis and Haptotaxis of Human and Rabbit Mesenchymal Stem Cells in a Standardized Transmembrane Assay

Fibronectin, Vitronectin, and Collagen I Induce Chemotaxis and Haptotaxis of Human and Rabbit Mesenchymal Stem Cells in a Standardized Transmembrane Assay

Repair of porcine articular cartilage defect with a biphasic osteochondral composite

Hydrogels for the Repair of Articular Cartilage Defects

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