Motility and growth of human bone-marrow mesenchymal stem cells during <i>ex vivo</i> expansion in autologous serum

Kobayashi, Takaaki; Watanabe, Hideomi; Yanagawa, Takao; Tsutsumi, S.; Kayakabe, M.; Shinozaki, Tetsuya; Higuchi, Hajime; Takagishi, Kenji

doi:10.1302/0301-620x.87b10.16160

Cited by 69 publications

(56 citation statements)

References 21 publications

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“…Platelet factors such as platelet-derived growth factor, transforming growth factor, and epidermal growth factor that promote increased migration and proliferation of MSCs have been studied extensively [18 -20]. The effectiveness of human serum is based mainly on studies using autologous serum [16,21], whereas the reports on allogeneic serum have been contradictory [22,23]. Our aim was to compare the effectiveness of human serum and platelet factors in promoting MSC growth with FCS.…”

Section: Introductionmentioning

confidence: 99%

Human AB Serum and Thrombin-Activated Platelet-Rich Plasma Are Suitable Alternatives to Fetal Calf Serum for the Expansion of Mesenchymal Stem Cells from Adipose Tissue

et al. 2007

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Section: Introductionmentioning

confidence: 99%

Human AB Serum and Thrombin-Activated Platelet-Rich Plasma Are Suitable Alternatives to Fetal Calf Serum for the Expansion of Mesenchymal Stem Cells from Adipose Tissue

et al. 2007

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“…FBS also contains non-human antigens, such as N-glycolylneuraminic acid, which is an obstacle for its application in clinical cell therapy [15]. Human Serum (HS) has been introduced as an alternative for FBS that provide the required proteins stem cells with no risk of disease transmission and rejection [8,14,16].…”

Section: Introductionmentioning

confidence: 99%

Comparing the Effects of Human and Fetal Bovine Serum on Mesenchymal Stem Cells Under Oxidative Stress

Razban

Hosseinipour

Mahmoodi

et al. 2017

Journal of Advanced Medical Sciences and Applied Technologies

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Objectives: Stem cells are undifferentiated cells capable of creating different types of cell in the body. Stem cell proliferation often is performed in the culture medium supplemented with Fetal Bovine Serum (FBS). Unknown compounds in the FBS, risk of contamination and disease transmission encourages the researches toward finding an alternative to FBS. Several factors are involved in the Mesenchymal Stem Cells (MSCs) precocious death in the transplanted tissue environment. Oxidative Stress (OS) is one of the main causes of stem cell apoptosis in the initial days after transplantation. The aim of this study was to evaluate the effect of Human Serum (HS) on the viability and oxidative related enzymes in human Adipose tissue-Derived Stem Cells (ADSCs) under oxidative stress in comparison with FBS. Materials & Methods:Human serum were obtained from blood of a healthy donor persons, in respective intervals during few days. The ADSCs were isolated from lipolysis operation samples and their cuture media were supplemented with FBS or HS and different concentrations of H 2 O 2 as the oxidative agent. Results:The results showed that cell proliferation and viability of ADSCs under oxidative stress condition was significantly higher in the culture medium supplemented with HS in comparison with FBS supplemented medium (P<0.05). Conclusion:This study showed that FBS could be replaced by HS in MSC culture medium with improved effects on cell proliferation and oxidative related enzyme activity under oxidative Stress condition.

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“…It has been shown that autologous serum has a positive influence on MSC behavior. 30 For positively charged molecules in particular, it is possible to stick to the negatively charged surface by SiO groups of the bone substitute and therefore promote the remodeling process. 31 Fibrin could cover the bone substitute surface and therefore mask the SiO groups, so that the matrix change and induction of bone formation could not take place.…”

mentioning

confidence: 99%

Autologous serum improves bone formation in a primary stable silica-embedded nanohydroxyapatite bone substitute in combination with mesenchymal stem cells and rhBMP-2 in the sheep model

Boos

Weigand

Deschler

et al. 2014

IJN

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New therapeutic strategies are required for critical size bone defects, because the gold standard of transplanting autologous bone from an unharmed area of the body often leads to several severe side effects and disadvantages for the patient. For years, tissue engineering approaches have been seeking a stable, axially vascularized transplantable bone replacement suitable for transplantation into the recipient bed with pre-existing insufficient conditions. For this reason, the arteriovenous loop model was developed and various bone substitutes have been vascularized. However, it has not been possible thus far to engineer a primary stable and axially vascularized transplantable bone substitute. For that purpose, a primary stable silica-embedded nanohydroxyapatite (HA) bone substitute in combination with blood, bone marrow, expanded, or directly retransplanted mesenchymal stem cells, recombinant human bone morphogenetic protein 2 (rhBMP-2), and different carrier materials (fibrin, cell culture medium, autologous serum) was tested subcutaneously for 4 or 12 weeks in the sheep model. Autologous serum lead to an early matrix change during degradation of the bone substitute and formation of new bone tissue. The best results were achieved in the group combining mesenchymal stem cells expanded with 60 μg/mL rhBMP-2 in autologous serum. Better ingrowth of fibrovascular tissue could be detected in the autologous serum group compared with the control (fibrin). Osteoclastic activity indicating an active bone remodeling process was observed after 4 weeks, particularly in the group with autologous serum and after 12 weeks in every experimental group. This study clearly demonstrates the positive effects of autologous serum in combination with mesenchymal stem cells and rhBMP-2 on bone formation in a primary stable silica-embedded nano-HA bone grafting material in the sheep model. In further experiments, the results will be transferred to the sheep arteriovenous loop model in order to engineer an axially vascularized primary stable bone replacement in clinically relevant size for free transplantation.

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Motility and growth of human bone-marrow mesenchymal stem cells during ex vivo expansion in autologous serum

Cited by 69 publications

References 21 publications

Human AB Serum and Thrombin-Activated Platelet-Rich Plasma Are Suitable Alternatives to Fetal Calf Serum for the Expansion of Mesenchymal Stem Cells from Adipose Tissue

Human AB Serum and Thrombin-Activated Platelet-Rich Plasma Are Suitable Alternatives to Fetal Calf Serum for the Expansion of Mesenchymal Stem Cells from Adipose Tissue

Comparing the Effects of Human and Fetal Bovine Serum on Mesenchymal Stem Cells Under Oxidative Stress

Autologous serum improves bone formation in a primary stable silica-embedded nanohydroxyapatite bone substitute in combination with mesenchymal stem cells and rhBMP-2 in the sheep model

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