Motility of Fasciola hepatica miracidia assessed with a computer-assisted sperm analyser

Abstract

The motility parameters of Fasciola hepatica miracidia were assessed at different temperatures and times post-hatching using computer-assisted sperm analysis. Eggs were incubated at 22 °C or 25 °C for 14 days. Five motion parameters were evaluated at different incubation temperatures up to 10 h post-hatching. No differences were observed in the percentage that hatched after incubation at the two different temperatures. However, the straight-line velocity of miracidia following incubation at 22 °C was significantly different from that observed at 25 °C (P< 0.01). All miracidium motion parameters at different post-hatching temperatures showed an overall decrease at the end of the experiment. Those miracidia hatching from eggs incubated at 25 °C had a higher velocity of 1673.3 μm/s compared with 1553.3 μm/s at 22 °C. Velocity parameters increased as the post-hatching temperature increased from 22 °C to 37 °C.

Prevalence and seasonal variation of Fasciola hepatica in slaughtered cattle: the role of climate and environmental factors in Mexico

“…Geographic information systems (GIS) and remote sensing technologies have been useful for extracting data on environmental features to investigate their relationships with disease at known sites, as well as to areas where disease does not exist (Dutra et al ., 2010; Charlier et al ., 2014). The susceptibility of cattle to the effects of climate change is not restricted to tropical, arid or temperate zones, and recent environmental and climate changes due to global warming may alter the epidemiology, seasonality and geographical distribution of the free-living stages of this parasite, thereby increasing the risk of livestock populations to the disease (Villa-Mancera et al ., 2015; Charlier et al ., 2016; Villa-Mancera & Reynoso-Palomar, 2019b).…”

Bulk tank milk ELISA to detect IgG1 prevalence and clustering to determine spatial distribution and risk factors of Fasciola hepatica-infected herds in Mexico

“…At least 90 million people are at risk of infection, and between 2.4 and 17 million individuals are believed to be infected (Keiser and Utzinger, 2009; Cwiklinski et al ., 2016). Fasciola hepatica resurgence has been linked to climate change affecting the prevalence, intensity and spatial distribution of the liver fluke, and influencing the free-living larval stages and population dynamics of the snails that are its intermediate hosts (Villa-Mancera et al , 2015; Charlier et al , 2016). Economic losses due to fasciolosis are estimated to amount to > 3 billion US dollars annually (Spithill et al , 1999; Cwiklinski et al , 2016).…”