Motility of Pseudomonas aeruginosa contributes to SOS-inducible biofilm formation

Chellappa, Shakinah Twinkle; Maredia, Reshma; Phipps, Kara L.; Haskins, William E.; Weitao, Tao

doi:10.1016/j.resmic.2013.10.001

Cited by 22 publications

(14 citation statements)

References 34 publications

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“…Similar observations were made in P . aeruginosa , where flagellum related motility was repressed when the SOS response was induced by ciprofloxacin [ 9 ].…”

Section: Resultsmentioning

confidence: 99%

“…The formation of SOS-inducible biofilm in P . aeruginosa was related to motility as the initial event facilitating biofilm development [ 9 ]. The observed increase in biofilm formation associated with the lexA mutant, coupled with observed ability to undergo gliding, suggests a similar relationship in C .…”

Section: Resultsmentioning

confidence: 99%

“…coli [ 7 , 8 ]. Chellappa and co-workers [ 9 ] showed that induction of the SOS response represses flagellar motility but increases biofilm formation in Pseudomonas aeruginosa . Furthermore, the resistance of Bacillus subtilis spores to DNA double-strand breaks has been correlated to RecA and its accessory proteins [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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The SOS Response Master Regulator LexA Is Associated with Sporulation, Motility and Biofilm Formation in Clostridium difficile

et al. 2015

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The LexA regulated SOS network is a bacterial response to DNA damage of metabolic or environmental origin. In Clostridium difficile, a nosocomial pathogen causing a range of intestinal diseases, the in-silico deduced LexA network included the core SOS genes involved in the DNA repair and genes involved in various other biological functions that vary among different ribotypes. Here we describe the construction and characterization of a lexA ClosTron mutant in C. difficile R20291 strain. The mutation of lexA caused inhibition of cell division resulting in a filamentous phenotype. The lexA mutant also showed decreased sporulation, a reduction in swimming motility, greater sensitivity to metronidazole, and increased biofilm formation. Changes in the regulation of toxin A, but not toxin B, were observed in the lexA mutant in the presence of sub-inhibitory concentrations of levofloxacin. C. difficile LexA is, therefore, not only a regulator of DNA damage but also controls many biological functions associated with virulence.

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“…Similar observations were made in P . aeruginosa , where flagellum related motility was repressed when the SOS response was induced by ciprofloxacin [ 9 ].…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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The SOS Response Master Regulator LexA Is Associated with Sporulation, Motility and Biofilm Formation in Clostridium difficile

et al. 2015

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“…ljungdahlii were not differentially expressed (results not shown), but besides the core SOS genes, LexA also affects the expression of genes encoding for various other functions. For instance, a mutation in the lexA gene led to reduced flagellar motility and increased biofilm formation in Pseudomonas aeruginosa [66], as well as in C . difficile [31].…”

Section: Discussionmentioning

confidence: 99%

Biofilm Formation by Clostridium ljungdahlii Is Induced by Sodium Chloride Stress: Experimental Evaluation and Transcriptome Analysis

et al. 2017

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The acetogen Clostridium ljungdahlii is capable of syngas fermentation and microbial electrosynthesis. Biofilm formation could benefit both these applications, but was not yet reported for C. ljungdahlii. Biofilm formation does not occur under standard growth conditions, but attachment or aggregation could be induced by different stresses. The strongest biofilm formation was observed with the addition of sodium chloride. After 3 days of incubation, the biomass volume attached to a plastic surface was 20 times higher with than without the addition of 200 mM NaCl to the medium. The addition of NaCl also resulted in biofilm formation on glass, graphite and glassy carbon, the latter two being often used electrode materials for microbial electrosynthesis. Biofilms were composed of extracellular proteins, polysaccharides, as well as DNA, while pilus-like appendages were observed with, but not without, the addition of NaCl. A transcriptome analysis comparing planktonic (no NaCl) and biofilm (NaCl addition) cells showed that C. ljungdahlii coped with the salt stress by the upregulation of the general stress response, Na+ export and osmoprotectant accumulation. A potential role for poly-N-acetylglucosamines and D-alanine in biofilm formation was found. Flagellar motility was downregulated, while putative type IV pili biosynthesis genes were not expressed. Moreover, the gene expression analysis suggested the involvement of the transcriptional regulators LexA, Spo0A and CcpA in stress response and biofilm formation. This study showed that NaCl addition might be a valuable strategy to induce biofilm formation by C. ljungdahlii, which can improve the efficacy of syngas fermentation and microbial electrosynthesis applications.

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“…SOS can be induced by antibiotics not only via direct DNA damage but also via indirect and subsequent production of hydroxyl radicals [101,102] though they do not kill the bacteria [103]. SOS contributes to antibiotic-inducible bacterial biofilm formation [104-106] and vesiculation [107]. Moreover, convergent evolution has been proposed between SOS-inducible biofilm formation and tumor metastasis [106,108-111].…”

Section: Discussionmentioning

confidence: 99%

Viral and cellular SOS-regulated motor proteins: dsDNA translocation mechanisms with divergent functions

2014

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DNA damage attacks on bacterial cells have been known to activate the SOS response, a transcriptional response affecting chromosome replication, DNA recombination and repair, cell division and prophage induction. All these functions require double-stranded (ds) DNA translocation by ASCE hexameric motors. This review seeks to delineate the structural and functional characteristics of the SOS response and the SOS-regulated DNA translocases FtsK and RuvB with the phi29 bacteriophage packaging motor gp16 ATPase as a prototype to study bacterial motors. While gp16 ATPase, cellular FtsK and RuvB are similarly comprised of hexameric rings encircling dsDNA and functioning as ATP-driven DNA translocases, they utilize different mechanisms to accomplish separate functions, suggesting a convergent evolution of these motors. The gp16 ATPase and FtsK use a novel revolution mechanism, generating a power stroke between subunits through an entropy-DNA affinity switch and pushing dsDNA inward without rotation of DNA and the motor, whereas RuvB seems to employ a rotation mechanism that remains to be further characterized. While FtsK and RuvB perform essential tasks during the SOS response, their roles may be far more significant as SOS response is involved in antibiotic-inducible bacterial vesiculation and biofilm formation as well as the perspective of the bacteria-cancer evolutionary interaction.

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Motility of Pseudomonas aeruginosa contributes to SOS-inducible biofilm formation

Cited by 22 publications

References 34 publications

The SOS Response Master Regulator LexA Is Associated with Sporulation, Motility and Biofilm Formation in Clostridium difficile

The SOS Response Master Regulator LexA Is Associated with Sporulation, Motility and Biofilm Formation in Clostridium difficile

Biofilm Formation by Clostridium ljungdahlii Is Induced by Sodium Chloride Stress: Experimental Evaluation and Transcriptome Analysis

Viral and cellular SOS-regulated motor proteins: dsDNA translocation mechanisms with divergent functions

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